Thanks for the responses. Yes, my crystal did survive and I can see
density for my ligand.
Summary:
1. If the crystal survives, it’s fine. These are just different ways
of exposing the crystal to the ligand and do what works. The only
issue will be if you don’t see electron density for the ligand
The truth is in the map. Ergo, zap it and rationalize why it worked or not
later.
Cheers, BR
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Markus
Heckmann
Sent: Tuesday, January 24, 2017 6:23 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] add ligand solution onto
Dear all,
I wondered if it is OK to pipette ligand soln (X-CoA) *directly* to the
drop with crystal (1:1 ratio of protein:precipitant 2µl) instead of
dissolving it in precipitant solution and transferring the crystal to this
ligand containing precipitant solution. The crystals survive this as I
