Dear Xun,
Regarding your monomer vs dimer, theoretical vs observed crysol plots
- yes - they are significantly different.
If you focus at the very lowest q part of the curve - the deviation
there in your monomer plots indicate that there is a significant size
difference between your PX monomer
If you main point is dimer vs monomer, Guinier plots is in fact all you would
need, in my opinion according to what I have read so far...
A.
On 17 Jun 2012, at 13:01, David Briggs wrote:
Dear Xun,
Regarding your monomer vs dimer, theoretical vs observed crysol plots
- yes - they are
It might also be useful to go through the recent paper by Jacques et al
Acta cryst D68:620-6 (2012) towards the standardization of saxs data analysis,
diagnostics and reporting.
Best regards,
Savvas
On 17-jun.-2012, at 13:01, David Briggs drdavidcbri...@gmail.com wrote:
Dear Xun,
Hi Xun,
it is difficult to judge without seeing the P(r) plots, but seeing as
you have a dimer in your crystal structure and a dimer in your SAXS,
AND your Chi2 value seems reasonable for a good match between PX and
SAXS, I'd say you've got what you need.
A matching P(r) plot would be nice, but
Two cents -
A good deal of caution must be exercised when working with composite
particles such as a protein-DNA complex in SAXS because of the contrast
problem. Simply, protein and DNA scatter differently in x-rays, with a bias
towards the DNA component. As a result, experimental Rgs could
