Dear Skkannu,
since citrate is (a) necessary for crystal growth and stability and (b)
blocks the active site I am afraid you will have to find a completely
different crystal form. This means start screening all over again for
crystallization conditions of your enzyme in the presence of a
Dear Samy,
You mentioned that your enzyme crystallizes in 0.1M citric acid pH3.5 plus
25% PEG 3,350.
I wonder if you have tried to systematically scan for other carboxylic acids
as buffer/co-precipitating salts. I would suggest you to try the serie of
carboxylic acids, citrate, acetate, formate
