. As I remember,
GE healthcare's SEC manuals has recommended procedures on TP determination.
Zhijie
-Original Message-
From: Edward A. Berry
Sent: Saturday, June 29, 2013 7:43 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] off topic: good peak on gel filtration
Peter Hsu wrote:
Hi all
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Edward A.
Berry [ber...@upstate.edu]
Sent: Saturday, June 29, 2013 9:34 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] off topic: good peak on gel filtration
Thanks- guess I'm old-fashioned, using low-pressure columns.
So
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] off topic: good peak on gel filtration
Peter Hsu wrote:
Hi all,
I've generally always thought as long as the peak was symmetrical and not
too broad would suggest a good sample. However, looking at my previous
runs in the past, I've had peaks as narrow as 1.5
Peter Hsu wrote:
Hi all,
I've generally always thought as long as the peak was symmetrical and not too
broad would suggest a good sample. However, looking at my previous runs in the
past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200, or slightly broader
peaks with about 3mL (all
Subject: Re: [ccp4bb] off topic: good peak on gel filtration
Peter Hsu wrote:
Hi all,
I've generally always thought as long as the peak was symmetrical and not
too broad would suggest a good sample. However, looking at my previous
runs in the past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200
Hi Peter,
I won't claim to be an expert, however, there is a small textbook by Robert K.
Scopes called Protein Purification: Principles and Practice, that has very
good practical and theoretical information about column chromatography (among
other methods). I have found this book useful a
topic: good peak on gel filtration
Peter Hsu wrote:
Hi all,
I've generally always thought as long as the peak was symmetrical and not
too broad would suggest a good sample. However, looking at my previous
runs in the past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200, or
slightly broader peaks