Re: [ccp4bb] off topic: good peak on gel filtration

2013-07-27 Thread Edward A. Berry
. As I remember, GE healthcare's SEC manuals has recommended procedures on TP determination. Zhijie -Original Message- From: Edward A. Berry Sent: Saturday, June 29, 2013 7:43 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off topic: good peak on gel filtration Peter Hsu wrote: Hi all

Re: [ccp4bb] off topic: good peak on gel filtration

2013-07-02 Thread Gloria Borgstahl
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Edward A. Berry [ber...@upstate.edu] Sent: Saturday, June 29, 2013 9:34 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off topic: good peak on gel filtration Thanks- guess I'm old-fashioned, using low-pressure columns. So

Re: [ccp4bb] off topic: good peak on gel filtration

2013-07-01 Thread El Arnaout, Toufic
@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off topic: good peak on gel filtration Peter Hsu wrote: Hi all, I've generally always thought as long as the peak was symmetrical and not too broad would suggest a good sample. However, looking at my previous runs in the past, I've had peaks as narrow as 1.5

Re: [ccp4bb] off topic: good peak on gel filtration

2013-06-29 Thread Edward A. Berry
Peter Hsu wrote: Hi all, I've generally always thought as long as the peak was symmetrical and not too broad would suggest a good sample. However, looking at my previous runs in the past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200, or slightly broader peaks with about 3mL (all

Re: [ccp4bb] off topic: good peak on gel filtration

2013-06-29 Thread Zhijie Li
Subject: Re: [ccp4bb] off topic: good peak on gel filtration Peter Hsu wrote: Hi all, I've generally always thought as long as the peak was symmetrical and not too broad would suggest a good sample. However, looking at my previous runs in the past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200

Re: [ccp4bb] off topic: good peak on gel filtration

2013-06-29 Thread Michael Thompson
Hi Peter, I won't claim to be an expert, however, there is a small textbook by Robert K. Scopes called Protein Purification: Principles and Practice, that has very good practical and theoretical information about column chromatography (among other methods). I have found this book useful a

Re: [ccp4bb] off topic: good peak on gel filtration

2013-06-29 Thread Edward A. Berry
topic: good peak on gel filtration Peter Hsu wrote: Hi all, I've generally always thought as long as the peak was symmetrical and not too broad would suggest a good sample. However, looking at my previous runs in the past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200, or slightly broader peaks