Re: [Freesurfer] Global Signal Scaling in FSFAST Pipeline

2017-12-11 Thread Taylor, Johnmark
Never mind, found the answer online, sorry about that!

On Mon, Dec 11, 2017 at 2:13 PM, Taylor, Johnmark <
johnmarktay...@g.harvard.edu> wrote:

> Hello,
>
> When using preproc-sess and selxavg3-sess, how is global mean scaling
> handled? Is the session-wide (4D) mean subtracted, is each volume's mean
> subtracted, or neither?
>
> Thank you very much,
>
> JohnMark
>
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[Freesurfer] Global Signal Scaling in FSFAST Pipeline

2017-12-11 Thread Taylor, Johnmark
Hello,

When using preproc-sess and selxavg3-sess, how is global mean scaling
handled? Is the session-wide (4D) mean subtracted, is each volume's mean
subtracted, or neither?

Thank you very much,

JohnMark
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[Freesurfer] Clarification about "Per-Run" Motion Correction and Registration in Preproc-Sess

2017-12-07 Thread Taylor, Johnmark
Thanks once again for all the help on this. I am running the GLM in native
space, typically so that I can get the beta values to use in an MVPA
analysis. So it sounds like if you specify per-run motion-correction and
registration, and don't tell it to resample to anything (e.g., the MNI
brain or the surface), then there's no guarantee that the voxels will
properly line up if you want to do, for instance, a single-subject GLM in
the subject's native space? So in this case, per-session motion correction
would be better so that all the functional volumes in the session are
registered properly?


> Thank you, this is very helpful! A few quick followup questions:
>
> 1) If you don't specifically tell preproc-sess what space to resample
> too (e.g., by using the "-surface" or "-mni305" flags), does it
> automatically resample to the subject's anatomical volume, then?
I don't think it will do anything if you don't specify
>
> 2) If fmc.nii.gz is already registered to the subject's volume, then
> how come a registration file has to be specified when visualizing GLM
> results using tksurfer? If fmc.nii.gz is already resampled to
> anatomical space, then surely any GLM contrasts should be as well, no?
fmc is not registered to the anatomical, it is registered to the
template. The template is registered to the anat through the
registration file. The data are still in the native functional space.
>
> 3) If per-run motion correction and registration is specified in
> pre-proc sess, then there will be a different functional-->structural
> transformation for each run, correct?
Correct
> If this is the case, then when visualizing session-wide GLM results
> using tksurfer, which registration file should be specified when
> loading up the overlay, since there'll be a different registration
> file for each run?
In what space are you doing the GLM? Usually, the raw data are resampled
onto the surface (eg, fmc.fsaverage.lh.sm05.nii.gz), and the GLM is done
in this spae.so no registration is needed.
>
> Apologies for these (probably basic) questions, I just want to make
> sure I am crystal-clear on what preproc-sess is doing.
>
> Cheers, and many thanks,
>
> JohnMark
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Re: [Freesurfer] Clarification about "Per-Run" Motion Correction and Registration in Preproc-Sess

2017-12-06 Thread Taylor, Johnmark
Thank you, this is very helpful! A few quick followup questions:

1) If you don't specifically tell preproc-sess what space to resample too
(e.g., by using the "-surface" or "-mni305" flags), does it automatically
resample to the subject's anatomical volume, then?

2) If fmc.nii.gz is already registered to the subject's volume, then how
come a registration file has to be specified when visualizing GLM results
using tksurfer? If fmc.nii.gz is already resampled to anatomical space,
then surely any GLM contrasts should be as well, no?

3) If per-run motion correction and registration is specified in pre-proc
sess, then there will be a different functional-->structural transformation
for each run, correct? If this is the case, then when visualizing
session-wide GLM results using tksurfer, which registration file should be
specified when loading up the overlay, since there'll be a different
registration file for each run?

Apologies for these (probably basic) questions, I just want to make sure I
am crystal-clear on what preproc-sess is doing.

Cheers, and many thanks,

JohnMark

On Mon, Dec 4, 2017 at 3:20 PM, <freesurfer-requ...@nmr.mgh.harvard.edu>
wrote:

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> Today's Topics:
>
>1. Re: mri_binarize (Douglas N Greve)
>2. Re: Clarification about "Per-Run" Motion Correction and
>   Registration in Preproc-Sess (Douglas N Greve)
>3. Re: freesurfer design (Douglas N Greve)
>4. Re: subcortical structures volume (Douglas N Greve)
>5. Re: mri_vol2surf wrong hemisphere (Douglas N Greve)
>6. Re: Concatenating .mgh surface space functional MRI   runs
>   (Douglas N Greve)
>7. Re: QDEC and comparing two conditions (Douglas N Greve)
>8. Re: Combining parcellated regions within an atlas?
>   (Douglas N Greve)
>9. Re: Overlaying multiple results onto one surface (Douglas N Greve)
>   10. recon edit error (Nicole Orme)
>
>
> --
>
> Message: 1
> Date: Mon, 4 Dec 2017 12:30:33 -0500
> From: Douglas N Greve <gr...@nmr.mgh.harvard.edu>
> Subject: Re: [Freesurfer] mri_binarize
> To: freesurfer@nmr.mgh.harvard.edu
> Message-ID: <9d91f7ea-a55b-cd78-2b55-687cf2078...@nmr.mgh.harvard.edu>
> Content-Type: text/plain; charset=utf-8; format=flowed
>
> you can do it in two steps. First, use mri_binarize to create a mask of
> the structutures you want. Then use mri_mask to apply the mask to the
> aparc.DKT file
>
>
> On 12/03/2017 07:44 AM, John Anderson wrote:
> > Dear FS experts,
> > I want to use mri_binarize to create (binarized and free of CSF and
> > ventricles) mask from "aparc.DKTatlas+aseg". How can I substract CSF
> > and ventricles from teh atlas using mri_binarize?
> >
> > Thanks
> > John
> >
> >
> > ___
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> --
> Douglas N. Greve, Ph.D.
> MGH-NMR Center
> gr...@nmr.mgh.harvard.edu
> Phone Number: 617-724-2358
> Fax: 617-726-7422
>
> Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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> Outgoing: ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/
>
>
>
> --
>
> Message: 2
> Date: Mon, 4 Dec 2017 12:32:00 -0500
> From: Douglas N Greve <gr...@nmr.mgh.harvard.edu>
> Subject: Re: [Freesurfer] Clarification about "Per-Run" Motion
> Correction and Registration in Preproc-Sess
> To: freesurfer@nmr.mgh.harvard.edu
> Message-ID: <813a5a08-d27e-a2a6-8472-66f998aaa...@nmr.mgh.harvard.edu>
> Content-Type: text/plain; charset=utf-8; format=flowed
>
>
>
> On 12/03/2017 06:35 PM, Taylor, Johnmark wrote:
> > Hello,
> >
> > A few quick questions about the "Per-Run" option in preproc-sess. I
> > understand that in this option, each run's images are registered to
> > the middle timepoint of that run, rather than the first 

[Freesurfer] Clarification about "Per-Run" Motion Correction and Registration in Preproc-Sess

2017-12-03 Thread Taylor, Johnmark
Hello,

A few quick questions about the "Per-Run" option in preproc-sess. I
understand that in this option, each run's images are registered to the
middle timepoint of that run, rather than the first timepoint of the first
run. I was wondering, though:

1) Why is this the preferred option instead of per-session?
2) If motion-correction is only done internally to each run, then wouldn't
between-run motion throw things off if you wanted to do a GLM spanning the
whole scanning session (although I assume it'd be fine for single-run
GLMs)? What ensures that the images line up properly between runs?

Thank you very much,

JohnMark
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[Freesurfer] Fixed Effects Analysis on Single Subject's Brain

2017-09-05 Thread Taylor, Johnmark
Hello,

Quick question. If I scanned a single subject across several sessions and
want to do a fixed effects analysis on that subject's brain (without
registering to MNI or Talairach space), how do I go about doing this? I am
trying to us isxconcat-sess and then mri_glmfit, but I am not seeing an
option to simply do the analysis in native space instead of registering to
a standard brain. Is there a way to do this besides taking the end result
of the analysis and doing the reverse registration back to the subject's
brain?

JohnMark
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[Freesurfer] Combining Results of Per-Run GLM?

2017-08-13 Thread Taylor, Johnmark
Hello,

I am trying to do a GLM analysis for a study in which I scanned each
participant twice. Currently, to combine the two scanning sessions I am
combining the runs from the two sessions into a single session directory
and applying per-session motion correction, essentially treating all the
data as though it came from a single session. The issue is that when I do
this, the GLM analysis takes prohibitively long (with just 8 subjects, the
analysis has already been running for two weeks). To speed things up, I had
the idea of doing the runwise GLM (getting the betas and contrast values
for each run), then combining the runwise results to get t-values and
p-values for the whole session. However, I am not entirely sure how to do
this last bit; is there some kind of function that can be used to take all
the per-run t-values or effect sizes in a session and combine them to get
an aggregate t-value and p-value for the whole session, for a given
contrast? I believe FSL follows this analysis strategy (doing runwise
analyses then combining them into the whole session), so I was wondering
whether FSFAST had a way of doing the same.

Thank you very much,

JohnMark
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[Freesurfer] Strange Discrepancy Between Activation Location on Surface vs. Volume

2017-07-03 Thread Taylor, Johnmark
Hello,

I am running into an odd issue. In particular, I have run a GLM analysis in
*volume* space, and I am visualizing the results both on the surface using
tksurfer, and on the volume using tkmedit. In both cases, I am using the
register.dof6.dat file to do the registration. Oddly, when I visualize the
results on the *surface*, the activation patch falls on the medial surface
of the hemisphere, in the *precuneus*, whereas when I visualize the results
on the *volume*, the patch is in the *intraparietal sulcus* (which is where
we expect to see this particular activation). I have made a label out of
the activation in both cases and viewed it in the other space just to make
sure that it is in fact the same activation, and it is. Any idea what might
be going on? It seems to me that it can't be an issue with the BOLD volume
--> structural volume registration, since the activations look normal on
tkmedit. It seems to me, then, that it must have to do with the
relationship between the anatomical volume and the anatomical
surface--could this be where the problem lies? Maybe an imperfect surface
reconstruction?

Thank you very much,

JohnMark
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[Freesurfer] Retrieving Model R^2 From FSFAST GLM Output?

2017-06-12 Thread Taylor, Johnmark
Hello,

Is there a simple way to retrieve the R^2 of the GLM model fit for each
voxel from the output of FSFAST? I wonder if rho1.nii.gz might be relevant,
but I couldn't find any documentation on this particular file.

Thank you very much,

JohnMark
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Re: [Freesurfer] FSFAST Full Model Residuals?

2017-06-07 Thread Taylor, Johnmark
Actually, just noticed that this is an option in selxavg3-sess, never mind!

On Wed, Jun 7, 2017 at 9:07 PM, Taylor, Johnmark <
johnmarktay...@g.harvard.edu> wrote:

> Hello,
>
> I had a quick question. Is there a way to make FSFAST output a 4D volume
> of all the residuals? In other words: is there a way to see, for each voxel
> and timepoint, how much of the BOLD response was unexplained by the GLM? I
> know that it outputs a volume containing the residual variances, but I am
> interested in looking at the residuals in more detail. I see that there is
> a directory called "res", but for all of the GLMs I've run it appears to be
> empty...
>
> Thank you very much,
>
> JohnMark
>
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Re: [Freesurfer] Difference in Recon-All Output Between Freesurfer Versions?

2017-04-10 Thread Taylor, Johnmark
(the reason I did this was because the new Freesurfer version outputs
labels for perirhinal cortex as part of the reconstruction process, and we
were interested in using this ROI in our analyses; as an additional
question, is there a way of producing these anatomical labels without
reconstructing the surface from scratch?)

On Mon, Apr 10, 2017 at 5:46 PM, Taylor, Johnmark <
johnmarktay...@g.harvard.edu> wrote:

> Hello,
>
> Recently, I re-ran recon-all with Freesurfer version 5 on some subjects
> whose surfaces had previously been reconstructed with Freesurfer version 4.
> As a result, it appears that their surface reconstructions have subtly
> changed, such that the labels that were specified in the old reconstruction
> are all messed up in the new reconstruction (since the surfaces that they
> were once associated with have changed). Did recon-all change between
> Freesurfer versions, and if so, is there a way to go back to the old
> algorithm short of simply switching back to Freesurfer version 4? It is
> possible that I have accidentally changed something else and that's why the
> surface files are different, but I wanted to see if there were any changes
> in the reconstruction algorithm between versions that might have caused
> this.
>
> Thank you very much,
>
> JohnMark Taylor
>
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[Freesurfer] Difference in Recon-All Output Between Freesurfer Versions?

2017-04-10 Thread Taylor, Johnmark
Hello,

Recently, I re-ran recon-all with Freesurfer version 5 on some subjects
whose surfaces had previously been reconstructed with Freesurfer version 4.
As a result, it appears that their surface reconstructions have subtly
changed, such that the labels that were specified in the old reconstruction
are all messed up in the new reconstruction (since the surfaces that they
were once associated with have changed). Did recon-all change between
Freesurfer versions, and if so, is there a way to go back to the old
algorithm short of simply switching back to Freesurfer version 4? It is
possible that I have accidentally changed something else and that's why the
surface files are different, but I wanted to see if there were any changes
in the reconstruction algorithm between versions that might have caused
this.

Thank you very much,

JohnMark Taylor
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[Freesurfer] Selxavg3-sess Looking for (and can't find) "fmc.sm0"

2017-04-05 Thread Taylor, Johnmark
Hello,

I am getting an odd bug on selxavg3-sess. I am getting the error message,

*ERROR: cannot find [rest of file path]/fmc.sm0*

I am indicating zero smoothing in mkanalysis-sess ("-fwhm 0"). I am
confused because the output of preproc-sess for no smoothing is
fmc.nii.gz, *NOT
*fmc.sm0.nii.gz when doing analyses in native volume space, so I am not
sure why selxavg-sess is looking for the wrong file path. For reference,
here is my call to mkanalysis-sess:

mkanalysis-sess -analysis analysis_name -fsd bold -runlistfile runlist.txt
-native -nuisreg mcextreg 3 -fwhm 0 -event-related -nconditions 8
-paradigm spiralpara.par -spmhrf 0 -refeventdur 12 -TR 1.5
-polyfit 2 -nskip 8

I am using Freesurfer 5.3, if that helps. The analysis works fine when I
apply 3mm smoothing (I've done preprocessing for both the 0mm and 3mm
options).

Thank you very much, and cheers,

JohnMark
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[Freesurfer] Best way to run GLM on a single subject across multiple sessions?

2016-10-03 Thread Taylor, Johnmark
Hello,

I am looking to run a GLM on a single subject, where data was collected
across several different sessions. What is the best way to go about doing
this in the FSFAST pipeline? I was thinking that I should just 1) drop the
data for all the runs into a single BOLD directory as though they were from
a single session, and 2) let the registration and motion correction do as
well as they can. Is this a reasonable approach, and if so, are there any
settings in the preprocessing or GLM that would advisable (for instance, in
terms of running the GLM on the surface versus the volume, or doing the
registration per-run versus per-session)?

Thank you very much,

JohnMark
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[Freesurfer] Viewing Retinotopy In Freeview

2016-09-09 Thread Taylor, Johnmark
Hello,

I am thinking of switching from Tksurfer to Freeview, but I want to make
sure that retinotopy visualization still works well. Is it straightforward
to view phase-encoded displays? There are a few options on tksurfer
(complex color wheel, ability to set number of cycles and angle offset for
phase encoded display, ability to smooth overlay) that I am not able to
immediately find on freeview. Currently, to visualize retinotopy data we
are using the strategy of loading both the real and complex parts of the
display on tksurfer. Are there analogous ways of doing all these steps on
freeview as of yet, or are they still waiting to be implemented?

Thank you very much,

JohnMark
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[Freesurfer] Can't Load More Than Four Unique Overlays on TKSurfer From Command Line

2016-09-08 Thread Taylor, Johnmark
Hello,

I am trying to load five overlays on TKSurfer from the command line (actual
command attached). The problem is this: if I try to load five different
overlays, the fifth one will show up on Freesurfer as being identical to
the first one (for instance, in this example, the "all-slope" overlay will
appear visually identical to the "fs-slope" overlay, even though I've
verified that the files are different). Furthermore, if you try to load
six, then the fifth overlay will look the same as the first, the sixth will
look the same as the second, and so forth. If I manually add the overlays
from the menu in tksurfer this doesn't happen, but I'd prefer not to have
to do this. Any idea what could be going on? Apologies that the command is
a mess to read: in case it isn't clear, I am calling the overlay flag five
separate times, once for each desired overlay.

Thanks,

JohnMark

tksurfer green_ant lh inflated -gray -title green_ant_lh *-overlay*
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/glm_sm5_ps/fs-slope/sig.nii.gz
-orf
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/register.dof6.dat
-fminmax 2 5 *-overlay*
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/glm_sm5_ps/fo-slope/sig.nii.gz
-orf
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/register.dof6.dat
-fminmax 2 5 *-overlay*
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/glm_sm5_ps/ps-slope/sig.nii.gz
-orf
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/register.dof6.dat
-fminmax 2 5 *-overlay*
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/glm_sm5_ps/po-slope/sig.nii.gz
-orf
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/register.dof6.dat
-fminmax 2 5 *-overlay*
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/glm_sm5_ps/all-slope/sig.nii.gz
-orf
/ncf/vcn/jtaylor/mri-space/studies/supips_loccompare01/unpackdata/160805_xu_supips_loccompare01_sub01/bold/register.dof6.dat
-fminmax 2 5
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[Freesurfer] Running Retinotopy in FSFAST, Design Matrix has Empty Regressors

2016-07-29 Thread Taylor, Johnmark
Hello,

I am trying to run a retinotopy analysis in fsfast, using only polar angle.
However, when I try to run it, I am getting the error "Input to SVD must
not contain NaN or Inf." I have traced this error and it looks like the
design matrix X has 12 regressors (the first 12) that consist solely of
zeros. I am using the following commands:

mkanalysis-sess -analysis retinotopy -fsd bold -runlistfile runlist.txt

-native -funcstem fmc -nuisreg mcextreg 3

-retinotopy 36.4 -per-session -paradigm retino.par -inorm

-spmhrf 0 -TR .650 -polyfit 2 -nskip 19


selxavg3-sess -analysis retinotopy -sf sublist.txt -df sessdir

Any idea what the first 12 regressors in the design matrix might correspond
to in retinotopy analyses and why they'd be empty? I have looked at the
documentation and there doesn't seem to be a clear labeling of the
retinotopy design matrix anywhere, although perhaps I'm missing something.

Thank you very much,

JohnMark
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[Freesurfer] Running Several Versions of fsfast GLM In Parallel Leads to "Stale File Handles"

2016-07-25 Thread Taylor, Johnmark
Hello,

I have been trying to run several versions of the same GLM analysis, trying
out different parameters to see if they affect the results. For instance, I
am running the GLM with different levels of smoothing, per-session versus
per-run motion correction, etc. To speed this up, I am trying to do these
GLMs in parallel. However, when I do this, I run into a "stale file handle"
problem. As near as I can tell, this occurs because the analyses try to
read and write to the same log files at the same time. A typical error
message is
"/net/rcss2/srv/export/ncf_vcn/share_root/jtaylor/mri-space/studies/fpoinfips01/scripts/glm/log/preproc-160718_xu_loc01_sub02-bold.log:
Stale file handle". I have tried putting "-nolog" in the call to
selxavg3-sess, but this doesn't prevent the creation of log files when it
makes the sub-calls to the various pre-processing functions. Is there any
way around this? It would be rather slow to try to run the GLMs
sequentially, so I am wondering if anybody has figured out a way around
this problem.

Much obliged,

JohnMark
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