Re: [galaxy-dev] FreeBayes Installation Bug

2012-09-12 Thread Daniel Blankenberg
Hi Lance, Thank you for finding this issue and a bigger thanks for the patch. We've updated FreeBayes in the toolshed with your patch. Please let us know if you encounter further issues. Thanks! Dan On Sep 11, 2012, at 5:08 PM, Lance Parsons wrote: > Today I ran into a new issues with inst

[galaxy-dev] FreeBayes Installation Bug

2012-09-11 Thread Lance Parsons
Today I ran into a new issues with installing a specific version of Freebayes. It turns out to be an issue with how git deals with submodules, something I'm only just learning about myself. In order to ensure that FreeBayes is built with the correct versions of all of the various submodules f

Re: [galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-09-11 Thread Lance Parsons
Thanks for information Greg. Actually, it turned out to be two things causing my issues: 1) My copy of tool-data/sam_fa_indices.loc.sample was not owned by the galaxy user (though it was writable by that user). This caused the exception which caused the installation to get "stuck" at "cloning

Re: [galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-08-31 Thread Greg Von Kuster
Hi Lance, You'll need to execute the following sql manually in your Galaxy database. update tool_shed_repository set status = 'New' where status = 'Cloning'; Next, you'll have to update your production Galaxy instance code base to at least changeset 2531e085f262 (the human-readable changeset re

Re: [galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-08-31 Thread Lance Parsons
Thanks for the explanation. That makes sense. I went ahead and installed the new version along with dependencies in my development environments and all went well. Then in production, the installation seems to have failed and I'm now stuck with freebayes in the "cloning" status. I tried to de

Re: [galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-08-31 Thread Daniel Blankenberg
Hi Lance, This has to do with the way that the updating currently works. Since the migrated version is 0.0.2, the automatic update will only update that particular Tool version (newest version of 0.0.2). To get 0.0.3, you need to install it as a new tool. This way you are able to have both vers

Re: [galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-08-31 Thread Lance Parsons
Thanks for the quick turnaround Dan. Much appreciated. When I tried to test updating Freebayes from the toolshed, I get a message that no updates are available. Perhaps it takes some time before they are recognized? Or am I missing something? Lance Daniel Blankenberg wrote: Hi Lance, Tha

Re: [galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-08-31 Thread Daniel Blankenberg
Hi Lance, Thank you for the pull request. I think this is an excellent way to handle patches until the toolshed itself provides a better mechanism, particularly for the devteam owned repositories; other repository owners may prefer different methods of providing patches, but they can be contact

[galaxy-dev] FreeBayes bug - update to a toolshed tool wrapper

2012-08-30 Thread Lance Parsons
I have been using the FreeBayes tool in our local Galaxy instance for some time. As FreeBayes is a rapidly evolving tool, it has been very helpful to have Daniel Blankenberg work on keeping an updated wrapper. Recently, I've run into a rather troublesome bug in FreeBayes (https://github.com/e

Re: [galaxy-dev] Freebayes

2011-04-05 Thread Juan Carlos Perin
I just tried via command line and re-did the steps that Galaxy attempted on the same files. I got through the samtools sorting of the bam file without a problem, but then soon saw the same error when I tried executing freebayes itself: [perin@variome 000]$ freebayes --fasta-reference /share/a

Re: [galaxy-dev] Freebayes

2011-04-05 Thread Dannon Baker
Juan, I'm not sure of a likely cause of this error off the top of my head. Have you tried running Freebayes from the command line on the same data input? That might be an easy way to explore what particular options with the newer version of Freebayes that you have are causing issues, or perha

Re: [galaxy-dev] Freebayes

2011-04-04 Thread Juan Carlos Perin
This fix got me further, however I'm now getting the following error, which is now more confusing... [bam_sort_core] merging from 40 files... BGZF ERROR: unable to open file 1.0 BGZF ERROR: unable to open file 1.0 Could not open input BAM files Juan On Apr 1, 2011, at 1:53 PM, Dannon Baker wrot

Re: [galaxy-dev] Freebayes

2011-04-01 Thread Dannon Baker
Juan, What version of freebayes are you running? The freebayes configuration in the galaxy repository was written with the .4 series in mind, and it appears that the options have changed with the .6 series. We'll update the tool config, but in the meanwhile you could probably get it working a

[galaxy-dev] Freebayes

2011-04-01 Thread Juan Carlos Perin
I'm trying to run Freebayes SNP calling on a bam file from a BWA alignment of solid data. I keep getting the following error: [bam_sort_core] merging from 40 files... freebayes: invalid option -- Y did you mean --bam ? I'm not sure where this is set in the code to try to debug things. I'm won