Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Sridhar Srinivasan
:44 AM, Mahtab Mirmomeni m.mirmom...@student.unimelb.edu.au wrote: You can use htseq_bams_to_count_matrix repository by fubar in the test toolshed. On Wed, Aug 21, 2013 at 2:37 PM, Sridhar Srinivasan sridhar2bioi...@gmail.com wrote: Hi All, Thanks for the suggestions. I installed

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Sridhar Srinivasan
, Aug 21, 2013 at 4:05 PM, Sridhar Srinivasan sridhar2bioi...@gmail.com wrote: Hi All, I installed the toolshed htseq_bams_to_count_matrix repository , i gave the gene model gtf file(ex: tss.gtf file) Gene model (GFF) file to count reads over from your current history: in this section

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Sridhar Srinivasan
/fubar/htseq_bams_to_count_matrix/f69b55c71ae5/INSTALLATION.log On Wed, Aug 21, 2013 at 4:38 PM, Sridhar Srinivasan sridhar2bioi...@gmail.com wrote: Hi Ross, i Installed through admin interface . for pysam and freetype it showed new... and rest installed.. Thanks On Wed, Aug 21

[galaxy-dev] DESeq for Galaxy

2013-08-20 Thread Sridhar Srinivasan
Dear users, Could anybody suggest the valid DESeq toolshed for RNA sequencing??? Thanks Sridhar ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists,

Re: [galaxy-dev] DESeq for Galaxy

2013-08-20 Thread Sridhar Srinivasan
Sure .. Thanks for the reply.. On Tue, Aug 20, 2013 at 12:33 PM, Bjoern Gruening bjoern.gruen...@gmail.com wrote: Hi Sridhar, we are developing a DESeq2 tool at the moment, if you want to join get in touch with me. Its developed here:

Re: [galaxy-dev] DESeq for Galaxy

2013-08-20 Thread Sridhar Srinivasan
Hi All, Thanks for the suggestions. I installed the Toolshed ( Differential_Count (version 0.21) ). It asks for the input matrix - rows are contigs, columns are counts for each sample. Any idea on how to generate this matrix file?? Thanks On Tue, Aug 20, 2013 at 12:53 PM, Mahtab Mirmomeni

Re: [galaxy-dev] cummerBund installation

2013-08-12 Thread Sridhar Srinivasan
?? Your suggestions are very much Apperiacted... Thank you Sridhar On Fri, Jul 12, 2013 at 6:29 PM, Jeremy Goecks jeremy.goe...@emory.eduwrote: The toolshed has a wrapper for cummerbund: http://toolshed.g2.bx.psu.edu/view/jjohnson/cummerbund On Jul 12, 2013, at 12:03 AM, sridhar srinivasan

Re: [galaxy-dev] GATK

2013-07-29 Thread Sridhar Srinivasan
--standard_min_confidence_threshold_for_calling 30.0 --standard_min_confidence_threshold_for_emitting 30.0' Thanks Sridhar On Tue, Jun 11, 2013 at 1:01 PM, sridhar srinivasan sridhar2bioi...@gmail.com wrote: Hello Linda, Thanks for your suggestions. i removed the line from xml file but now i am getting different

[galaxy-dev] GATK fasta file missing ERROR

2013-07-24 Thread Sridhar Srinivasan
Dear Developers, I am GATK (beta ) Unified Genotyper module to get the indels and SNP. I placed the jar file in (/galaxy-dist/tool-data/shared/jars/gatk) and i link the reference genome in .loc file (gatk_sorted_picard_index.loc). I could see the Name i mentioned in the .loc file is appearing in

[galaxy-dev] Visualization in galaxy

2013-07-11 Thread sridhar srinivasan
Dear Developers, I could see the visualize icon in the History page below the output file.. Actually i am trying to plot the graph between the column 4 and 8 from the output.. I tried using the visualize option, But takes long time to and i could'nt get the plot.. Please suggest , how to get

Re: [galaxy-dev] Visualization in galaxy

2013-07-11 Thread sridhar srinivasan
more details as to what's happening when you click the draw button? Thanks, Carl On Thu, Jul 11, 2013 at 5:22 AM, sridhar srinivasan sridhar2bioi...@gmail.com wrote: Dear Developers, I could see the visualize icon in the History page below the output file.. Actually i am trying to plot

[galaxy-dev] cummerBund installation

2013-07-11 Thread sridhar srinivasan
Dear Developers, Could you please tell me the possibilities of using the R package cummeRband for visualization of RNA-seq data in galaxy?? Thanks Sridhar ___ Please keep all replies on the list by using reply all in your mail client. To

[galaxy-dev] Using Jar files in Galaxy

2013-07-11 Thread sridhar srinivasan
Dear Developers, I am using java jar files for softwares like fastqc, GATK. For running these tools i install the softare and set path in bash_profile file. My doubt is i placed the jar files in galaxy dist folder ( galaxy/galaxy-dist/tool-data/shared/jars/FastQC/fastqc ) is this enough to

Re: [galaxy-dev] Using Jar files in Galaxy

2013-07-11 Thread sridhar srinivasan
. That's the way of the future for all tool wrappers, so a good thing to start using. Plenty of docs on the wiki and examples in the test toolshed. On Fri, Jul 12, 2013 at 2:34 PM, sridhar srinivasan sridhar2bioi...@gmail.com wrote: Dear Developers, I am using java jar files for softwares

[galaxy-dev] Fwd: GATK fasta file not found ERROR

2013-06-26 Thread sridhar srinivasan
Dear Developers, I am using GATK for first time. I am doing Targeted reseq Analysis. while i am running Unified Genotyper in Galaxy interface. it gives error message as mentioned below.. ERROR MESSAGE: The fasta file you specified (/tmp/tmp-gatk-WBrGTf) does not exist. But i places the Fasta

Re: [galaxy-dev] GATK

2013-06-11 Thread sridhar srinivasan
files. [image: Inactive hide details for sridhar srinivasan ---06/06/2013 06:30:48 AM---Dear Developers, I am running GATK for first time. In]sridhar srinivasan ---06/06/2013 06:30:48 AM---Dear Developers, I am running GATK for first time. In galaxy i use Unified Genotyper. I am From: sridhar

[galaxy-dev] GATK

2013-06-06 Thread sridhar srinivasan
Dear Developers, I am running GATK for first time. In galaxy i use Unified Genotyper. I am getting error as below mentioned. # ERROR -- # ERROR A USER ERROR has occurred (version 2.5-2-gf57256b):

[galaxy-dev] mapping Summary from Bowtie

2013-06-03 Thread sridhar srinivasan
Dear Developers, I am running Bowtie2 through galaxy interface, previously it gives the the mapping summary and convert the format to sam.. but now it directly convert the fastq file to bam and doesnt give the mapping summary. how to get the mapping summary from bowtie2 using galaxy interface..

[galaxy-dev] Genome not listed

2013-05-29 Thread sridhar srinivasan
Dear developers, 1. I recently installed galaxy locally. now i am trying to run bowtie2. In reference genome section the genome is not listed. 2. Is there any way yo secure our data without using the Proxy(nginx). I Appericiate your suggestions. Thanks Sridhar

Re: [galaxy-dev] Error in installing Galaxy.

2013-05-21 Thread sridhar srinivasan
:24 PM, sridhar srinivasan wrote: Hi , I am getting error during Installing galaxy locally. Traceback (most recent call last): File /illumina/apps/galaxy/galaxy-**dist/lib/galaxy/webapps/** galaxy/buildapp.py, line 35, in app_factory app = UniverseApplication( global_conf

Re: [galaxy-dev] Error in installing Galaxy.

2013-05-21 Thread sridhar srinivasan
None None Thanks Sridhar On Tue, May 21, 2013 at 3:08 PM, Hans-Rudolf Hotz h...@fmi.ch wrote: On 05/21/2013 11:22 AM, sridhar srinivasan wrote: Hi Rudolf, Thanks for the reply.. Could you please tell how to check and set the PostgreSQL .. I provide the correct username and password

Re: [galaxy-dev] Error in installing Galaxy.

2013-05-21 Thread sridhar srinivasan
localhost -U galaxy and this should prompt for the password (again, I am not an PostgreSQL expert...) Regards, Hans-Rudolf On 05/21/2013 01:00 PM, sridhar srinivasan wrote: Hello Rudolf, I tried with the IP address it gave the error as Is the server running on host 192.168.1.1

[galaxy-dev] Fwd: History page not opening

2013-05-15 Thread sridhar srinivasan
Hi, In backend i am getting this message 192.168.1.5 - - [15/May/2013:09:32:12 +0600] GET / HTTP/1.1 200 - http://192.168.1.5- -/ Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.18) Gecko/20110616 Red Hat/3.6-1.el5_6 Firefox/3.6.18 Thanks Sridhar On 5/14/13, sridhar srinivasan sridhar2bioi

[galaxy-dev] Web Page Problem

2013-05-15 Thread sridhar srinivasan
Dear Developers, After installing galaxy, the history part of the webpage is not opening and it shows Content Encoding Error The page you are trying to view cannot be shown because it uses an invalid or unsupported form of compression. * Please contact the website owners to inform them

[galaxy-dev] history page not opening

2013-05-13 Thread sridhar srinivasan
Dear Developers, After configuring the galaxy.. i could'nt see the history column in the web page.. it shows the error as Content Encoding Error The page you are trying to view cannot be shown because it uses an invalid or unsupported form of compression. Best Sridhar

[galaxy-dev] History page not opening

2013-05-13 Thread sridhar srinivasan
Hi after installing galaxy, i could'nt see the history page in right end of galaxy web. it shows Content Encoding Error The page you are trying to view cannot be shown because it uses an invalid or unsupported form of compression. * Please contact the website owners to inform them of

Re: [galaxy-dev] history page not opening

2013-05-13 Thread sridhar srinivasan
using any kind of proxy server in front of it? -- James Taylor, Assistant Professor, Biology/CS, Emory University On Mon, May 13, 2013 at 2:53 AM, sridhar srinivasan sridhar2bioi...@gmail.com wrote: Dear Developers, After configuring the galaxy.. i could'nt see the history column

Re: [galaxy-dev] History page not opening

2013-05-13 Thread sridhar srinivasan
, sridhar srinivasan sridhar2bioi...@gmail.com wrote: Hi after installing galaxy, i could'nt see the history page in right end of galaxy web. it shows Content Encoding Error The page you are trying to view cannot be shown because it uses an invalid or unsupported form of compression

Re: [galaxy-dev] Error in installing Galaxy.

2013-05-09 Thread sridhar srinivasan
...@gmail.com wrote: On Thu, May 9, 2013 at 12:21 AM, sridhar srinivasan sridhar2bioi...@gmail.com wrote: and it is working.. but i could'nt connect to the webpage http://127.0.0.1:8080/ from another system in same network.. By default, galaxy will only be serving on 127.0.0.1 (localhost

[galaxy-dev] Error in installing Galaxy.

2013-05-08 Thread sridhar srinivasan
Hi , I am getting error during Installing galaxy locally. Traceback (most recent call last): File /illumina/apps/galaxy/galaxy-dist/lib/galaxy/webapps/galaxy/buildapp.py, line 35, in app_factory app = UniverseApplication( global_conf = global_conf, **kwargs ) File

Re: [galaxy-dev] Error in installing Galaxy.

2013-05-08 Thread sridhar srinivasan
the same error has been reported before: http://lists.bx.psu.edu/**pipermail/galaxy-dev/2010-May/**002624.htmlhttp://lists.bx.psu.edu/pipermail/galaxy-dev/2010-May/002624.html Regards, Hans-Rudolf On 05/08/2013 01:24 PM, sridhar srinivasan wrote: Hi , I am getting error during

Re: [galaxy-dev] (no subject)

2013-05-06 Thread sridhar srinivasan
, sridhar srinivasan sridhar2bioi...@gmail.com wrote: Dear All, i am installing galaxy first time. i face problem while installing the python eggs. while running the Run.sh command it gives as /home/sbsuser/.python-eggs/pysam-0.4.2_kanwei_b10f6e722e9a-py2.6-linux-x86_64-ucs4.egg-tmp

[galaxy-dev] (no subject)

2013-05-03 Thread sridhar srinivasan
Dear All, i am installing galaxy first time. i face problem while installing the python eggs. while running the Run.sh command it gives as /home/sbsuser/.python-eggs/pysam-0.4.2_kanwei_b10f6e722e9a-py2.6-linux-x86_64-ucs4.egg-tmp/csamtools.so:6: RuntimeWarning: __builtin__.file size changed,