[galaxy-dev] reference genomes missing

2013-07-31 Thread Gerald Bothe
I installed a local instance of GALAXY following the instructions at "Get Galaxy ..." in the Wiki. It installed ok, but when I tried the Bowtie2 mapper with a test data set, I noticed that the reference genomes are missing. How can I get the reference genomes (e.g. latest version of mouse and hu

[galaxy-dev] rsync questions

2013-08-03 Thread Gerald Bothe
Here are a couple of questions on rsync:   1. the amount of files for hg19 and mm9 is extremely different (over 600 vs. 88 Gb) - why is that so? 2. if I list the directories for hg19 and mm9, there seems to be a group of files for bowtie2 in hg19, but not in mm9 - where are the ones for mm9? 3. g

[galaxy-dev] rsync questions

2013-08-05 Thread Gerald Bothe
Here are a couple of questions on rsync: 1. the amount of files for hg19 and mm9 is extremely different (over 600 vs. 88 Gb) - why is that so? They are both mammalian genomes ... are there redundant files in hg19, is mm9 incomplete...? 2. if I list the directories for hg19 and mm9, there seems t

[galaxy-dev] getting large files into a local instance of Galaxy

2013-08-05 Thread Gerald Bothe
Trying out a local instance of Galaxy. Small files can be uploaded with "browse.." but this works only for files under 2Gb. Is there a folder in a local copy of galaxy-dist where I can copy a large file and have it show up in the Get Data/Upload File/Files uploaded via FTP window so that I can p

Re: [galaxy-dev] Local installation: Questions about using rsync to retrieve a genome

2013-08-06 Thread Gerald Bothe
I'll leave the rsync question to the experts - worked for me, but I don't know why it worked for me (linux/ubuntu laptop, not networked) and not for you .   About the filenames: You can download a file listing to text.txt by   .../galaxy-dist$ rsync -avzP rsync://datacache.g2.bx.psu.edu/indexes >

Re: [galaxy-dev] Scaling and hardware requirements

2013-09-11 Thread Gerald Bothe
Can I put in a similar question on top of this: How much resources do you need for re-sequencing of a mammalian genome (assembly and variant detection), one job at a time? E.g. how much RAM  etc. if I want the re-sequencing SAM file of a 30-fold coverage be done in 48 hours?   Gerald Gerald

Re: [galaxy-dev] Scaling and hardware requirements

2013-09-12 Thread Gerald Bothe
(one single lab) or a production instance (whole institute or more) would be great   ... or could anybody give their own 2 cents?   Gerald   > > From: Jeremy Goecks >To: Gerald Bothe >Cc: Nikos Sidiropoulos ; Peter Cock >; "" >Sent