Re: [galaxy-dev] Getting bowtie2 and tophat2 to work on galaxy on amazon aws cloud

2013-10-14 Thread Stanislas Werfel
Thanks. I tried both File1 and 2 of SRR618542. Here's one of the links: ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR618/SRR618542/SRR618542_1.fastq.gz On Oct 14, 2013, at 8:01 PM, Vipin TS wrote: > > - bowtie2 does not show any built-in reference genomes > > > by default Cloudman didn't ship the ge

Re: [galaxy-dev] Getting bowtie2 and tophat2 to work on galaxy on amazon aws cloud

2013-10-14 Thread Vipin TS
> - bowtie2 does not show any built-in reference genomes > > by default Cloudman didn't ship the genome index for bowtie2. Bowtie2 use its own index set. Usage: bowtie2-build [option] genome.fa index_basename and add the entries in "bowtie2_indices.loc". Please make sure that the fields are tab de

Re: [galaxy-dev] Getting bowtie2 and tophat2 to work on galaxy on amazon aws cloud

2013-10-14 Thread Stanislas Werfel
Thanks for the quick answer. Could access bowtie2 from command line without a problem. Found that I had to include a line pointing to the wrapper in tool_conf.xml. It wasn't there by default. Now two new problems: - bowtie2 does not show any built-in reference genomes - when I try to import a

Re: [galaxy-dev] Getting bowtie2 and tophat2 to work on galaxy on amazon aws cloud

2013-10-14 Thread Vipin TS
Hi Stanislas, Please check the PATH variable and make sure that you can access bowtie2 from command line and then restart the Galaxy service through cloudman interface. I think that should work. Vipin > > I'm trying to run Galaxy on an Amazon AWS cloud using the normal Cloudman > distribution (

[galaxy-dev] Getting bowtie2 and tophat2 to work on galaxy on amazon aws cloud

2013-10-14 Thread Stanislas Werfel
Dear all, I'm trying to run Galaxy on an Amazon AWS cloud using the normal Cloudman distribution (ami-118bfc78). The instatiated Galaxy however does not contain Bowtie2 and Tophat2, even after updating through the Cloudman Admin section. Although the wrappers are there in the tools directory, w