Re: [galaxy-dev] Problem with installing MAF cached dataset

2013-09-12 Thread Leon Mei
Hi Dan,

Thanks for the solution! It did help me to identify a missing index
file. I think the indexing run of that particular config maf file
failed for some reason. Since I don't expect much interesting thing on
that, I just remove that for now.

==
hmei@ubuntu:/mnt/galaxyTools/galaxy-central/scripts/tools/maf$ python
check_loc_file.py ../../../tool-data/maf_index.loc
/usr/local/lib/python2.7/dist-packages/distribute-0.6.49-py2.7.egg/pkg_resources.py:1025:
UserWarning: /home/hmei/.python-eggs is writable by group/others and
vulnerable to attack when used with get_resource_filename. Consider a
more secure location (set with .set_extraction_path or the
PYTHON_EGG_CACHE environment variable).
  warnings.warn(msg, UserWarning)
Line 6 is invalid: [Errno 2] No such file or directory:
'/mnt/galaxyIndices/alignments/maf/chr11_gl000202_random.maf.index'
hmei@ubuntu:/mnt/galaxyTools/galaxy-central/scripts/tools/maf$
==

So now I have my MAF functions working. Thanks again!

Leon

On Wed, Sep 11, 2013 at 9:18 PM, Daniel Blankenberg  wrote:
> Hi Leon,
>
> Can you see if there is any info reported reported by running the MAF 
> consistency checking script at scripts/tools/maf/check_loc_file.py 
> path/to/maf_index.loc?
>
> You'll need to make sure that required dependencies (e.g. galaxy_root/lib and 
> galaxy_root/eggs) are included in your PYTHONPATH.
>
>
> As far as cutting down a MAF to only contain the set of desired species and 
> depending upon your needs, you can use the tools included with Galaxy, or 
> alternatively the maf_thread_for_species.py script in bx-python.
>
>
>
> Thanks for using Galaxy,
>
>
> Dan


-- 
Hailiang (Leon) Mei
Netherlands Bioinformatics Center
BioAssist NGS Taskforce
 - http://ngs.nbic.nl
Skype: leon_meiMobile: +31 6 41709231
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Re: [galaxy-dev] Problem with installing MAF cached dataset

2013-09-11 Thread Daniel Blankenberg
Hi Leon,

Can you see if there is any info reported reported by running the MAF 
consistency checking script at scripts/tools/maf/check_loc_file.py 
path/to/maf_index.loc?

You'll need to make sure that required dependencies (e.g. galaxy_root/lib and 
galaxy_root/eggs) are included in your PYTHONPATH.


As far as cutting down a MAF to only contain the set of desired species and 
depending upon your needs, you can use the tools included with Galaxy, or 
alternatively the maf_thread_for_species.py script in bx-python.



Thanks for using Galaxy,


Dan


On Sep 11, 2013, at 2:10 PM, Leon Mei wrote:

> Hi Dan,
> 
> Here are the output:
> ==
> hmei@ubuntu:~$ ls -lah /mnt/galaxyIndices/alignments/maf/
> total 236G
> drwxrwsr-x 2 postgres postgres 4.0K Sep 10 00:08 .
> drwxrwsr-x 4 postgres postgres 4.0K Sep  9 16:27 ..
> -rw-rw-r-- 1 postgres postgres  13G Oct 31  2009 chr10.maf
> -rw-rw-r-- 1 postgres postgres 189M Sep  9 17:29 chr10.maf.index
> -rw-rw-r-- 1 postgres postgres 189M Sep  8 21:35 chr11_gl000202_random.maf
> -rw-rw-r-- 1 postgres postgres  12G Oct 31  2009 chr11.maf
> -rw-rw-r-- 1 postgres postgres 184M Sep  9 18:07 chr11.maf.index
> -rw-rw-r-- 1 postgres postgres  12G Oct 31  2009 chr12.maf
> -rw-rw-r-- 1 postgres postgres 186M Sep  9 18:31 chr12.maf.index
> -rw-rw-r-- 1 postgres postgres 8.7G Oct 31  2009 chr13.maf
> -rw-rw-r-- 1 postgres postgres 135M Sep  9 18:49 chr13.maf.index
> -rw-rw-r-- 1 postgres postgres 8.3G Oct 31  2009 chr14.maf
> -rw-rw-r-- 1 postgres postgres 125M Sep  9 19:05 chr14.maf.index
> -rw-rw-r-- 1 postgres postgres 7.3G Oct 31  2009 chr15.maf
> -rw-rw-r-- 1 postgres postgres 112M Sep  9 19:19 chr15.maf.index
> -rw-rw-r-- 1 postgres postgres 7.1G Oct 31  2009 chr16.maf
> -rw-rw-r-- 1 postgres postgres 108M Sep  9 19:32 chr16.maf.index
> -rw-rw-r-- 1 postgres postgres  39M Oct 31  2009 chr17_ctg5_hap1.maf
> -rw-rw-r-- 1 postgres postgres 456K Sep  9 19:45 chr17_ctg5_hap1.maf.index
> -rw-rw-r-- 1 postgres postgres 753K Oct 31  2009 chr17_gl000203_random.maf
> -rw-rw-r-- 1 postgres postgres  45K Sep  9 19:45 
> chr17_gl000203_random.maf.index
> -rw-rw-r-- 1 postgres postgres 5.1M Oct 31  2009 chr17_gl000204_random.maf
> -rw-rw-r-- 1 postgres postgres 101K Sep  9 19:45 
> chr17_gl000204_random.maf.index
> -rw-rw-r-- 1 postgres postgres 1.6M Oct 31  2009 chr17_gl000205_random.maf
> -rw-rw-r-- 1 postgres postgres  33K Sep  9 19:45 
> chr17_gl000205_random.maf.index
> -rw-rw-r-- 1 postgres postgres 968K Oct 31  2009 chr17_gl000206_random.maf
> -rw-rw-r-- 1 postgres postgres  24K Sep  9 19:45 
> chr17_gl000206_random.maf.index
> -rw-rw-r-- 1 postgres postgres 7.3G Oct 31  2009 chr17.maf
> -rw-rw-r-- 1 postgres postgres 112M Sep  9 19:45 chr17.maf.index
> -rw-rw-r-- 1 postgres postgres 265K Oct 31  2009 chr18_gl000207_random.maf
> -rw-rw-r-- 1 postgres postgres 3.5K Sep  9 19:59 
> chr18_gl000207_random.maf.index
> -rw-rw-r-- 1 postgres postgres 7.0G Oct 31  2009 chr18.maf
> -rw-rw-r-- 1 postgres postgres 106M Sep  9 19:59 chr18.maf.index
> -rw-rw-r-- 1 postgres postgres 4.2K Oct 31  2009 chr19_gl000208_random.maf
> -rw-rw-r-- 1 postgres postgres  193 Sep  9 20:05 
> chr19_gl000208_random.maf.index
> -rw-rw-r-- 1 postgres postgres 2.6M Oct 31  2009 chr19_gl000209_random.maf
> -rw-rw-r-- 1 postgres postgres  54K Sep  9 20:05 
> chr19_gl000209_random.maf.index
> -rw-rw-r-- 1 postgres postgres 3.5G Oct 31  2009 chr19.maf
> -rw-rw-r-- 1 postgres postgres  48M Sep  9 20:05 chr19.maf.index
> -rw-rw-r-- 1 postgres postgres 4.1M Oct 31  2009 chr1_gl000191_random.maf
> -rw-rw-r-- 1 postgres postgres 108K Sep  9 20:05 
> chr1_gl000191_random.maf.index
> -rw-rw-r-- 1 postgres postgres  22M Oct 31  2009 chr1_gl000192_random.maf
> -rw-rw-r-- 1 postgres postgres 376K Sep  9 20:06 
> chr1_gl000192_random.maf.index
> -rw-rw-r-- 1 postgres postgres  21G Oct 31  2009 chr1.maf
> -rw-rw-r-- 1 postgres postgres 330M Sep  9 17:13 chr1.maf.index
> -rw-rw-r-- 1 postgres postgres 5.6G Oct 31  2009 chr20.maf
> -rw-rw-r-- 1 postgres postgres  83M Sep  9 20:59 chr20.maf.index
> -rw-rw-r-- 1 postgres postgres 342K Oct 31  2009 chr21_gl000210_random.maf
> -rw-rw-r-- 1 postgres postgres  31K Sep  9 21:05 
> chr21_gl000210_random.maf.index
> -rw-rw-r-- 1 postgres postgres 2.9G Oct 31  2009 chr21.maf
> -rw-rw-r-- 1 postgres postgres  38M Sep  9 21:05 chr21.maf.index
> -rw-rw-r-- 1 postgres postgres 2.8G Oct 31  2009 chr22.maf
> -rw-rw-r-- 1 postgres postgres  37M Sep  9 21:10 chr22.maf.index
> -rw-rw-r-- 1 postgres postgres  23G Oct 31  2009 chr2.maf
> -rw-rw-r-- 1 postgres postgres 355M Sep  9 20:49 chr2.maf.index
> -rw-rw-r-- 1 postgres postgres  19G Oct 31  2009 chr3.maf
> -rw-rw-r-- 1 postgres postgres 291M Sep  9 21:45 chr3.maf.index
> -rw-rw-r-- 1 postgres postgres 9.1M Oct 31  2009 chr4_ctg9_hap1.maf
> -rw-rw-r-- 1 postgres postgres 150K Sep  9 22:16 chr4_ctg9_hap1.maf.index
> -rw-rw-r-- 1 postgres postgres 4.8M Oct 31  2009 chr4_gl000193_random.maf
> -rw-rw-r-- 1 postgres postgres 101K Sep  9 22:16 
> chr4_

Re: [galaxy-dev] Problem with installing MAF cached dataset

2013-09-11 Thread Leon Mei
Hi Dan,

Here are the output:
==
hmei@ubuntu:~$ ls -lah /mnt/galaxyIndices/alignments/maf/
total 236G
drwxrwsr-x 2 postgres postgres 4.0K Sep 10 00:08 .
drwxrwsr-x 4 postgres postgres 4.0K Sep  9 16:27 ..
-rw-rw-r-- 1 postgres postgres  13G Oct 31  2009 chr10.maf
-rw-rw-r-- 1 postgres postgres 189M Sep  9 17:29 chr10.maf.index
-rw-rw-r-- 1 postgres postgres 189M Sep  8 21:35 chr11_gl000202_random.maf
-rw-rw-r-- 1 postgres postgres  12G Oct 31  2009 chr11.maf
-rw-rw-r-- 1 postgres postgres 184M Sep  9 18:07 chr11.maf.index
-rw-rw-r-- 1 postgres postgres  12G Oct 31  2009 chr12.maf
-rw-rw-r-- 1 postgres postgres 186M Sep  9 18:31 chr12.maf.index
-rw-rw-r-- 1 postgres postgres 8.7G Oct 31  2009 chr13.maf
-rw-rw-r-- 1 postgres postgres 135M Sep  9 18:49 chr13.maf.index
-rw-rw-r-- 1 postgres postgres 8.3G Oct 31  2009 chr14.maf
-rw-rw-r-- 1 postgres postgres 125M Sep  9 19:05 chr14.maf.index
-rw-rw-r-- 1 postgres postgres 7.3G Oct 31  2009 chr15.maf
-rw-rw-r-- 1 postgres postgres 112M Sep  9 19:19 chr15.maf.index
-rw-rw-r-- 1 postgres postgres 7.1G Oct 31  2009 chr16.maf
-rw-rw-r-- 1 postgres postgres 108M Sep  9 19:32 chr16.maf.index
-rw-rw-r-- 1 postgres postgres  39M Oct 31  2009 chr17_ctg5_hap1.maf
-rw-rw-r-- 1 postgres postgres 456K Sep  9 19:45 chr17_ctg5_hap1.maf.index
-rw-rw-r-- 1 postgres postgres 753K Oct 31  2009 chr17_gl000203_random.maf
-rw-rw-r-- 1 postgres postgres  45K Sep  9 19:45 chr17_gl000203_random.maf.index
-rw-rw-r-- 1 postgres postgres 5.1M Oct 31  2009 chr17_gl000204_random.maf
-rw-rw-r-- 1 postgres postgres 101K Sep  9 19:45 chr17_gl000204_random.maf.index
-rw-rw-r-- 1 postgres postgres 1.6M Oct 31  2009 chr17_gl000205_random.maf
-rw-rw-r-- 1 postgres postgres  33K Sep  9 19:45 chr17_gl000205_random.maf.index
-rw-rw-r-- 1 postgres postgres 968K Oct 31  2009 chr17_gl000206_random.maf
-rw-rw-r-- 1 postgres postgres  24K Sep  9 19:45 chr17_gl000206_random.maf.index
-rw-rw-r-- 1 postgres postgres 7.3G Oct 31  2009 chr17.maf
-rw-rw-r-- 1 postgres postgres 112M Sep  9 19:45 chr17.maf.index
-rw-rw-r-- 1 postgres postgres 265K Oct 31  2009 chr18_gl000207_random.maf
-rw-rw-r-- 1 postgres postgres 3.5K Sep  9 19:59 chr18_gl000207_random.maf.index
-rw-rw-r-- 1 postgres postgres 7.0G Oct 31  2009 chr18.maf
-rw-rw-r-- 1 postgres postgres 106M Sep  9 19:59 chr18.maf.index
-rw-rw-r-- 1 postgres postgres 4.2K Oct 31  2009 chr19_gl000208_random.maf
-rw-rw-r-- 1 postgres postgres  193 Sep  9 20:05 chr19_gl000208_random.maf.index
-rw-rw-r-- 1 postgres postgres 2.6M Oct 31  2009 chr19_gl000209_random.maf
-rw-rw-r-- 1 postgres postgres  54K Sep  9 20:05 chr19_gl000209_random.maf.index
-rw-rw-r-- 1 postgres postgres 3.5G Oct 31  2009 chr19.maf
-rw-rw-r-- 1 postgres postgres  48M Sep  9 20:05 chr19.maf.index
-rw-rw-r-- 1 postgres postgres 4.1M Oct 31  2009 chr1_gl000191_random.maf
-rw-rw-r-- 1 postgres postgres 108K Sep  9 20:05 chr1_gl000191_random.maf.index
-rw-rw-r-- 1 postgres postgres  22M Oct 31  2009 chr1_gl000192_random.maf
-rw-rw-r-- 1 postgres postgres 376K Sep  9 20:06 chr1_gl000192_random.maf.index
-rw-rw-r-- 1 postgres postgres  21G Oct 31  2009 chr1.maf
-rw-rw-r-- 1 postgres postgres 330M Sep  9 17:13 chr1.maf.index
-rw-rw-r-- 1 postgres postgres 5.6G Oct 31  2009 chr20.maf
-rw-rw-r-- 1 postgres postgres  83M Sep  9 20:59 chr20.maf.index
-rw-rw-r-- 1 postgres postgres 342K Oct 31  2009 chr21_gl000210_random.maf
-rw-rw-r-- 1 postgres postgres  31K Sep  9 21:05 chr21_gl000210_random.maf.index
-rw-rw-r-- 1 postgres postgres 2.9G Oct 31  2009 chr21.maf
-rw-rw-r-- 1 postgres postgres  38M Sep  9 21:05 chr21.maf.index
-rw-rw-r-- 1 postgres postgres 2.8G Oct 31  2009 chr22.maf
-rw-rw-r-- 1 postgres postgres  37M Sep  9 21:10 chr22.maf.index
-rw-rw-r-- 1 postgres postgres  23G Oct 31  2009 chr2.maf
-rw-rw-r-- 1 postgres postgres 355M Sep  9 20:49 chr2.maf.index
-rw-rw-r-- 1 postgres postgres  19G Oct 31  2009 chr3.maf
-rw-rw-r-- 1 postgres postgres 291M Sep  9 21:45 chr3.maf.index
-rw-rw-r-- 1 postgres postgres 9.1M Oct 31  2009 chr4_ctg9_hap1.maf
-rw-rw-r-- 1 postgres postgres 150K Sep  9 22:16 chr4_ctg9_hap1.maf.index
-rw-rw-r-- 1 postgres postgres 4.8M Oct 31  2009 chr4_gl000193_random.maf
-rw-rw-r-- 1 postgres postgres 101K Sep  9 22:16 chr4_gl000193_random.maf.index
-rw-rw-r-- 1 postgres postgres 2.2M Oct 31  2009 chr4_gl000194_random.maf
-rw-rw-r-- 1 postgres postgres 112K Sep  9 22:16 chr4_gl000194_random.maf.index
-rw-rw-r-- 1 postgres postgres  17G Oct 31  2009 chr4.maf
-rw-rw-r-- 1 postgres postgres 257M Sep  9 22:16 chr4.maf.index
-rw-rw-r-- 1 postgres postgres  17G Oct 31  2009 chr5.maf
-rw-rw-r-- 1 postgres postgres 255M Sep  9 22:47 chr5.maf.index
-rw-rw-r-- 1 postgres postgres  44M Oct 31  2009 chr6_apd_hap1.maf
-rw-rw-r-- 1 postgres postgres 496K Sep  9 23:18 chr6_apd_hap1.maf.index
-rw-rw-r-- 1 postgres postgres  96M Oct 31  2009 chr6_cox_hap2.maf
-rw-rw-r-- 1 postgres postgres 1.1M Sep  9 23:18 chr6_cox_hap2.maf.index
-rw-rw-r-- 1 postgres postgres  86M Oct 31  2009 chr6_dbb_hap3.maf

Re: [galaxy-dev] Problem with installing MAF cached dataset

2013-09-11 Thread Daniel Blankenberg
Hi Leon,

What is the output of "ls -lah /mnt/galaxyIndices/alignments/maf/"?

Also, you may what to use additional sub-directory structure for your file 
locations in case you want to add more alignments later, e.g., 
/mnt/galaxyIndices/hg19/maf/10_way_multiz/ or similiar

Also, note that although you are only indexing (for extraction) for the 
specified listed species, the alignments will contain all of the species 
present in the original file (you list 46way multiZ as the source).


Thanks for using Galaxy,

Dan


On Sep 10, 2013, at 6:44 PM, Leon Mei wrote:

> Dear colleagues,
> 
> I am having some trouble installing MAF datasets at our server. When I
> run "Extract MAF blocks given a set of genomic intervals", I got this
> error message:
> 
> "Fatal Error: The MAF source specified (10_WAY_MULTIZ_hg19) appears to
> be invalid."
> 
> Below is what I did by following the wiki page:
> http://wiki.galaxyproject.org/Admin/Datatypes/Add%20MAFs
> 
> 1, download all mafs for hg19 from
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/multiz46way/maf/
> 
> 2, index them using "maf_build_index.py --species=hg19,panTro2,
> gorGor1,ponAbe2,papHam1,calJac1,tarSyr1,micMur1,otoGar1,rheMac2
> $file". Our user requested primates only.
> 
> 3, Here is my maf_index.loc file:
> ==
> 10-way multiZ (hg19)10_WAY_MULTIZ_hg19
> hg19,panTro2,gorGor1,ponAbe2,papHam1,calJac1,tarSyr1,micMur1,otoGar1,rheMac2
>  hg19,panTro2,gorGor1,ponAbe2,papHam1,calJac1,tarSyr1,micMur1,otoGar1,rheMac2
>   /mnt/galaxyIndices/alignments/maf/chr1.maf,/mnt/galaxyIndices/alignments/m
>   
> af/chr10.maf,/mnt/galaxyIndices/alignments/maf/chr11.maf,/mnt/galaxyIndices/alignments/maf/chr11_gl000202_random.maf,/mnt/galaxyIndices/ali
>   
> gnments/maf/chr12.maf,/mnt/galaxyIndices/alignments/maf/chr13.maf,/mnt/galaxyIndices/alignments/maf/chr14.maf,/mnt/galaxyIndices/alignments
>   
> /maf/chr15.maf,/mnt/galaxyIndices/alignments/maf/chr16.maf,/mnt/galaxyIndices/alignments/maf/chr17.maf,/mnt/galaxyIndices/alignments/maf/ch
>   
> r17_ctg5_hap1.maf,/mnt/galaxyIndices/alignments/maf/chr17_gl000203_random.maf,/mnt/galaxyIndices/alignments/maf/chr17_gl000204_random.maf,/
>   
> mnt/galaxyIndices/alignments/maf/chr17_gl000205_random.maf,/mnt/galaxyIndices/alignments/maf/chr17_gl000206_random.maf,/mnt/galaxyIndices/a
>   
> lignments/maf/chr18.maf,/mnt/galaxyIndices/alignments/maf/chr18_gl000207_random.maf,/mnt/galaxyIndices/alignments/maf/chr19.maf,/mnt/galaxy
>   
> Indices/alignments/maf/chr19_gl000208_random.maf,/mnt/galaxyIndices/alignments/maf/chr19_gl000209_random.maf,/mnt/galaxyIndices/alignments/
>   
> maf/chr1_gl000191_random.maf,/mnt/galaxyIndices/alignments/maf/chr1_gl000192_random.maf,/mnt/galaxyIndices/alignments/maf/chr2.maf,/mnt/gal
>   
> axyIndices/alignments/maf/chr20.maf,/mnt/galaxyIndices/alignments/maf/chr21.maf,/mnt/galaxyIndices/alignments/maf/chr21_gl000210_random.maf
>   
> ,/mnt/galaxyIndices/alignments/maf/chr22.maf,/mnt/galaxyIndices/alignments/maf/chr3.maf,/mnt/galaxyIndices/alignments/maf/chr4.maf,/mnt/gal
>   
> axyIndices/alignments/maf/chr4_ctg9_hap1.maf,/mnt/galaxyIndices/alignments/maf/chr4_gl000193_random.maf,/mnt/galaxyIndices/alignments/maf/c
>   
> hr4_gl000194_random.maf,/mnt/galaxyIndices/alignments/maf/chr5.maf,/mnt/galaxyIndices/alignments/maf/chr6.maf,/mnt/galaxyIndices/alignments
>   
> /maf/chr6_apd_hap1.maf,/mnt/galaxyIndices/alignments/maf/chr6_cox_hap2.maf,/mnt/galaxyIndices/alignments/maf/chr6_dbb_hap3.maf,/mnt/galaxyI
>   
> ndices/alignments/maf/chr6_mann_hap4.maf,/mnt/galaxyIndices/alignments/maf/chr6_mcf_hap5.maf,/mnt/galaxyIndices/alignments/maf/chr6_qbl_hap
>   
> 6.maf,/mnt/galaxyIndices/alignments/maf/chr6_ssto_hap7.maf,/mnt/galaxyIndices/alignments/maf/chr7.maf,/mnt/galaxyIndices/alignments/maf/chr
>   
> 7_gl000195_random.maf,/mnt/galaxyIndices/alignments/maf/chr8.maf,/mnt/galaxyIndices/alignments/maf/chr8_gl000196_random.maf,/mnt/galaxyIndi
>   ces/alignments/maf/chr8_gl000197_random.maf
> ==
> 
> Any hint on which step is wrong or missing is highly appreciate!
> 
> Thanks,
> Leon
> 
> 
> 
> 
> 
> -- 
> Hailiang (Leon) Mei
> Netherlands Bioinformatics Center
> BioAssist NGS Taskforce
> - http://ngs.nbic.nl
> Skype: leon_meiMobile: +31 6 41709231
> ___
> Please keep all replies on the list by using "reply all"
> in your mail client.  To manage your subscriptions to this
> and other Galaxy lists, please use the interface at:
>  http://lists.bx.psu.edu/
> 
> To search Galaxy mailing lists use the unified search at:
>  http://galaxyproject.org/search/mailinglists/


___
Please keep all replies on the list by using "reply all"
in your mail client.  To manage your subscriptions to this
and other Galaxy lists, please use the interface at:
  http://lists.bx.psu.edu/

To search Galaxy mailing lists use the unified search at:
  http://galaxyproject.org/search/mailinglists/


[galaxy-dev] Problem with installing MAF cached dataset

2013-09-11 Thread Leon Mei
Dear colleagues,

I am having some trouble installing MAF datasets at our server. When I
run "Extract MAF blocks given a set of genomic intervals", I got this
error message:

"Fatal Error: The MAF source specified (10_WAY_MULTIZ_hg19) appears to
be invalid."

Below is what I did by following the wiki page:
http://wiki.galaxyproject.org/Admin/Datatypes/Add%20MAFs

1, download all mafs for hg19 from
http://hgdownload.cse.ucsc.edu/goldenPath/hg19/multiz46way/maf/

2, index them using "maf_build_index.py --species=hg19,panTro2,
gorGor1,ponAbe2,papHam1,calJac1,tarSyr1,micMur1,otoGar1,rheMac2
$file". Our user requested primates only.

3, Here is my maf_index.loc file:
==
10-way multiZ (hg19)10_WAY_MULTIZ_hg19
hg19,panTro2,gorGor1,ponAbe2,papHam1,calJac1,tarSyr1,micMur1,otoGar1,rheMac2
  hg19,panTro2,gorGor1,ponAbe2,papHam1,calJac1,tarSyr1,micMur1,otoGar1,rheMac2
   /mnt/galaxyIndices/alignments/maf/chr1.maf,/mnt/galaxyIndices/alignments/m
   
af/chr10.maf,/mnt/galaxyIndices/alignments/maf/chr11.maf,/mnt/galaxyIndices/alignments/maf/chr11_gl000202_random.maf,/mnt/galaxyIndices/ali
   
gnments/maf/chr12.maf,/mnt/galaxyIndices/alignments/maf/chr13.maf,/mnt/galaxyIndices/alignments/maf/chr14.maf,/mnt/galaxyIndices/alignments
   
/maf/chr15.maf,/mnt/galaxyIndices/alignments/maf/chr16.maf,/mnt/galaxyIndices/alignments/maf/chr17.maf,/mnt/galaxyIndices/alignments/maf/ch
   
r17_ctg5_hap1.maf,/mnt/galaxyIndices/alignments/maf/chr17_gl000203_random.maf,/mnt/galaxyIndices/alignments/maf/chr17_gl000204_random.maf,/
   
mnt/galaxyIndices/alignments/maf/chr17_gl000205_random.maf,/mnt/galaxyIndices/alignments/maf/chr17_gl000206_random.maf,/mnt/galaxyIndices/a
   
lignments/maf/chr18.maf,/mnt/galaxyIndices/alignments/maf/chr18_gl000207_random.maf,/mnt/galaxyIndices/alignments/maf/chr19.maf,/mnt/galaxy
   
Indices/alignments/maf/chr19_gl000208_random.maf,/mnt/galaxyIndices/alignments/maf/chr19_gl000209_random.maf,/mnt/galaxyIndices/alignments/
   
maf/chr1_gl000191_random.maf,/mnt/galaxyIndices/alignments/maf/chr1_gl000192_random.maf,/mnt/galaxyIndices/alignments/maf/chr2.maf,/mnt/gal
   
axyIndices/alignments/maf/chr20.maf,/mnt/galaxyIndices/alignments/maf/chr21.maf,/mnt/galaxyIndices/alignments/maf/chr21_gl000210_random.maf
   
,/mnt/galaxyIndices/alignments/maf/chr22.maf,/mnt/galaxyIndices/alignments/maf/chr3.maf,/mnt/galaxyIndices/alignments/maf/chr4.maf,/mnt/gal
   
axyIndices/alignments/maf/chr4_ctg9_hap1.maf,/mnt/galaxyIndices/alignments/maf/chr4_gl000193_random.maf,/mnt/galaxyIndices/alignments/maf/c
   
hr4_gl000194_random.maf,/mnt/galaxyIndices/alignments/maf/chr5.maf,/mnt/galaxyIndices/alignments/maf/chr6.maf,/mnt/galaxyIndices/alignments
   
/maf/chr6_apd_hap1.maf,/mnt/galaxyIndices/alignments/maf/chr6_cox_hap2.maf,/mnt/galaxyIndices/alignments/maf/chr6_dbb_hap3.maf,/mnt/galaxyI
   
ndices/alignments/maf/chr6_mann_hap4.maf,/mnt/galaxyIndices/alignments/maf/chr6_mcf_hap5.maf,/mnt/galaxyIndices/alignments/maf/chr6_qbl_hap
   
6.maf,/mnt/galaxyIndices/alignments/maf/chr6_ssto_hap7.maf,/mnt/galaxyIndices/alignments/maf/chr7.maf,/mnt/galaxyIndices/alignments/maf/chr
   
7_gl000195_random.maf,/mnt/galaxyIndices/alignments/maf/chr8.maf,/mnt/galaxyIndices/alignments/maf/chr8_gl000196_random.maf,/mnt/galaxyIndi
   ces/alignments/maf/chr8_gl000197_random.maf
==

Any hint on which step is wrong or missing is highly appreciate!

Thanks,
Leon





-- 
Hailiang (Leon) Mei
Netherlands Bioinformatics Center
BioAssist NGS Taskforce
 - http://ngs.nbic.nl
Skype: leon_meiMobile: +31 6 41709231
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