Re: [galaxy-dev] Pick-you-own columns in BLAST+ tabular output

2014-03-14 Thread Peter Cock
On Tue, Mar 4, 2014 at 12:19 PM, Peter Cock p.j.a.c...@googlemail.com wrote:
 On Tue, Mar 4, 2014 at 10:35 AM, Björn Grüning bjoern.gruen...@gmail.com 
 wrote:
 Hi Peter,

 Hello all,

 I've mentioned before that a forthcoming update to the BLAST+
 Galaxy wrappers would be adding a most customisable output
 option with pick-your-own columns. This is available to test now
 from the Test Tool Shed, along with other enhancements like
 more masking tools and an update to BLAST+ 2.2.29:

 http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus

 ...


 Some community testing/comments would be appreciated.

 I tested it briefly on my test instance and it looks all fine and
 seems to work :)

 Thanks, looking forward to see it in MainTS.
 Bjoern

 Great - thanks. I'm about to update our local production
 server, and barring any problems will push this BLAST+
 update to the main Tool Shed later this week.

This is now live, with a regression about output formats
caught and fixed:

http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus

Peter

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Re: [galaxy-dev] Pick-you-own columns in BLAST+ tabular output

2014-03-04 Thread Björn Grüning

Hi Peter,


Hello all,

I've mentioned before that a forthcoming update to the BLAST+
Galaxy wrappers would be adding a most customisable output
option with pick-your-own columns. This is available to test now
from the Test Tool Shed, along with other enhancements like
more masking tools and an update to BLAST+ 2.2.29:

http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus

(This will be released as the ncbi_blast_plus wrappers v0.1.0,
and includes recent contributions from Jim Johnson and
Björn Grüning - thank you both):

Previously (for tabular output) we've only offered the standard
12 column output (-outfmt 6), and an extended set which has
grown to currently include 25 columns.

The new custom column mode offers the 12 standard columns,
and the extended set (making 25 columns), plus other (to me
less interesting) identifiers, some other miscellaneous
columns, and the taxonomy columns (44 columns in all):

[image: Inline image 1]
[image: Inline image 2]

As previously discussed, the list of columns has been grouped
- the first two groups reflect the standard 12 columns and the
columns used in Galaxy's (default) 25 column extended tabular
output.

The remaining columns I have split into three groups (assorted
identifiers, miscellaneous, and taxonomy) which I hope is self
explanatory.

Note that this does *not* allow you pick the column order - they
will be produced in the order shown. The underlying BLAST+
tools would support this, but the Galaxy GUI would I feel be
too complicated.


I think that is fine, I can't imagine of any real use case where that is 
needed.



Some community testing/comments would be appreciated.


I tested it briefly on my test instance and it looks all fine and seems 
to work :)


Thanks, looking forward to see it in MainTS.
Bjoern



Thanks,

Peter


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Re: [galaxy-dev] Pick-you-own columns in BLAST+ tabular output

2014-01-17 Thread Bossers, Alex
Peter,
We will definitely give them a try. I never understood why the tabular outputs 
did have an option to include the defline/hit decription. Just for that we 
needed to use the cloggy XML output and parse that into a table again.
Conserved header names are more useful to us than having column numbers. But 
either is fine with us.
Alex

-Oorspronkelijk bericht-
Van: galaxy-dev-boun...@lists.bx.psu.edu 
[mailto:galaxy-dev-boun...@lists.bx.psu.edu] Namens Peter Cock
Verzonden: woensdag 15 januari 2014 11:54
Aan: galaxy-dev@lists.bx.psu.edu
Onderwerp: [galaxy-dev] Pick-you-own columns in BLAST+ tabular output

Hello all,

For those interested in more flexible tabular output from BLAST+, please try 
out the wrappers on the *Test* Tool Shed which I have also just updated to wrap 
BLAST+ 2.2.29 as well:

http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus

One open question is should the interface for picking columns include the 
current column numbers or not? e.g.

http://dev.list.galaxyproject.org/attachment/4662771/0/blast-pyo-columns-split.png

(Screenshot from this thread last month, where opinion was split) 
http://dev.list.galaxyproject.org/Including-descriptions-etc-in-extended-BLAST-tabular-output-tc4662704.html

In future work we'll add some of the new columns added in the
BLAST+ 2.2.28 release like taxonomy fields - but this needs a
bit of thought about how to handle the taxonomy database (i.e.
should we support multiple versions for full reproducibility, or just use the 
current version on disk regardless). Most of the time updates to the NCBI 
taxonomy will simply be additions, which should be harmless to reproducibility 
- but sometimes taxa can be rearranged.
https://github.com/peterjc/galaxy_blast/issues/20
https://github.com/peterjc/galaxy_blast/issues/21

Regards,

Peter
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