Re: [galaxy-dev] microarray NGS

2011-11-18 Thread Ivan Merelli

Dear Eric, thank you for your answer.
Your workaround works for the pheno file.

A related question is: the affybatch and the
eset file formats require to upload two files
(rispectively the pheno and the eset/affybatch).

Is it normal to have finally only
the pheno one in the History?

I.


Hi Ivan,

I can provide an explanation about the first part at least.  You are seeing that 
##failed message due to a bug in Galaxy core code that I've previously raised on this 
list.  The path you see there is where the .pheno file should (and does) end up after the tool is 
done.  However, the code that generates the ##failed message is getting called before 
the .pheno file has been moved to that path from its temporary location.  Hence, it can't be found 
there at that point in execution time.

While requiring a workaround is not ideal, a very simple one does exist.  In the history item for 
your uploaded dataset, click on the pencil (Edit attributes) and then click the button 
that says Auto-detect.

Best,
Eric


From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] 
on behalf of Ivan Merelli [ivan.mere...@itb.cnr.it]
Sent: Wednesday, November 09, 2011 7:12 AM
To: galaxy-dev@lists.bx.psu.edu
Subject: [galaxy-dev] microarray  NGS

Hi Galaxy Users,

I'm interested in integrating microarray and NGS data.
My problem concerns the uploading of an affybatch object
in Galaxy (I have a local instance of galaxy, but exacly
the same problem is present in http://main.g2.bx.psu.edu)

After processing the upload, the only message in the
green output data box of the history is something like:
##failed to find
/galaxy/main_database/files/003/251/dataset_3251699_files/rexpression.pheno
Can you help me in understanding what's wrong?

In particular the data which I'm trying to upload are
created with rexpression (incidentally, is it still
supported or novel versions are attended?), because
this package gives my some problems and I'm trying
to upload manually some results (which seems ok on
the disk) to see what is happening.

Cheers,
Ivan

P.s.
Maybe some other packages exists to deal with microarray
data in Galaxy? e.g. retrieving data from GEO/ArrayExpress,
normalizing them, show in a matrix differential expressed
genes to join/confront them with novel NGS experiment...
I know it's a lot of stuff..

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Re: [galaxy-dev] microarray NGS

2011-11-09 Thread Ross
Hi Ivan,

 In particular the data which I'm trying to upload are
 created with rexpression (incidentally, is it still
 supported or novel versions are attended?), because

Unfortunately, rexpression is not supported at present- the source is
readily available from bitbucket but the original author (me) stopped
working on it.
I'd be happy to hand the source on to others working in that space -
it's in bitbucket.
I think many people would agree that integrating gene expression and
NGS variation data is a very interesting goal but I'm not aware of any
tools to do that.

On Wed, Nov 9, 2011 at 7:10 AM, Ivan Merelli ivan.mere...@itb.cnr.it wrote:
 Hi Galaxy Users,

 I'm interested in integrating microarray and NGS data.
 My problem concerns the uploading of an affybatch object
 in Galaxy (I have a local instance of galaxy, but exacly
 the same problem is present in http://main.g2.bx.psu.edu)

 After processing the upload, the only message in the
 green output data box of the history is something like:
 ##failed to find
 /galaxy/main_database/files/003/251/dataset_3251699_files/rexpression.pheno
 Can you help me in understanding what's wrong?

 In particular the data which I'm trying to upload are
 created with rexpression (incidentally, is it still
 supported or novel versions are attended?), because
 this package gives my some problems and I'm trying
 to upload manually some results (which seems ok on
 the disk) to see what is happening.

 Cheers,
 Ivan

 P.s.
 Maybe some other packages exists to deal with microarray
 data in Galaxy? e.g. retrieving data from GEO/ArrayExpress,
 normalizing them, show in a matrix differential expressed
 genes to join/confront them with novel NGS experiment...
 I know it's a lot of stuff..

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Please keep all replies on the list by using reply all
in your mail client.  To manage your subscriptions to this
and other Galaxy lists, please use the interface at:

  http://lists.bx.psu.edu/