Re: [galaxy-user] calculating percent coverage over the target genome

2011-07-28 Thread David Matthews
Hi Jen, Many thanks for this, on a related subject do you know of a way to correct a FASTA file on the basis of a pileup (or even just on the BAM file)? Best Wishes, David. __ Dr David A. Matthews Senior Lecturer in Virology Room E49 Department of Cellular and

[galaxy-user] query

2011-07-28 Thread archana bhardwaj
Respected sir I am using fastaq to convert fasta and quality file. But after sometime i am getting an error and m not able to fix it. please guide me what sholud i do?? ___ The Galaxy User list should be used for the discussion of Galaxy

Re: [galaxy-user] symbolic link to bowtie

2011-07-28 Thread Jennifer Jackson
Hello Luciano, You probably need to be root to write to /bin (often done by leading the command string with a sudo). It depends on how your system is set up. But you don't need to put bowtie at the top level /bin, it doesn't really belong there as a higher global function. It can go in a

Re: [galaxy-user] query

2011-07-28 Thread vasu punj
It appear that you are talking about RNA-seq data analysis. If so please read a tutorial posted by Jeremy to get an idea how the work flow has to be used. I am sure folks would also like to know what error you are getting. --- On Thu, 7/28/11, archana bhardwaj archana2...@gmail.com wrote:

[galaxy-user] Galaxy News Page and RSS Feed

2011-07-28 Thread Dave Clements
Hello all, The new Galaxy wiki features a Galaxy News page and RSS feed for items of interest to the Galaxy community. The news page is a good way to get information out to the community and is less intrusive than posting to the Galaxy mailing lists. Anyone can create a news items by following

[galaxy-user] question about using bowtie

2011-07-28 Thread William Light
I am trying to use bowtie to assign reads to the s. Cerevisiae genome. I have data from paired end SOLiD sequencing with two unique six base pair barcodes. Can I use bowtie to make csfasta and qual files from my mixed original data split by bar code? I know I can use the trim option to remove