[galaxy-user] Extracting sequences for transcripts from reference genome

2013-04-08 Thread Lizex Husselmann
Dear Galaxy community I'm new to galaxy and would like to ask the following: I have trimmed, QC'ed my data received from Illumina HiScan SQ, paired and single end data. Mapped using Tophat, run cufflinks, cuffmerge and cuffdiff. I would like to analyze the gene_exp.diff file by extracting the

Re: [galaxy-user] Problem Loading History Pane

2013-02-16 Thread Lizex Husselmann
Dear all I have in my project single end reads (50 bp) for some samples and paired end reads (100 bp frw and rev) for the other samples. I had to re-sequence some of the samples of which I have paired-end reads. However the re-sequence data I receives is single-end reads of 250 bp. Tophat

[galaxy-user] Galaxy download

2012-10-02 Thread Lizex Husselmann
didn't open. Any suggestions? Kind regards Lizex Husselmann This message is confidential and may be covered by legal professional privilege. It must not be read, copied, disclosed or used in any other manner by any person other than the addressee(s). Unauthorised use, disclosure or copying

[galaxy-user] Galaxy-installed - Localhost Not opening

2012-08-17 Thread Lizex Husselmann
Hi all I've installed Galaxy on my Mac. The sh run.sh, run but the next step which is launching Galaxy (http://localhost:8080) on my web browser cannot connect. What could be the problem? Lizex This message is confidential and may be covered by legal professional privilege. It must not be

[galaxy-user] Cufflink error

2011-11-07 Thread Lizex Husselmann
Hi I've started an alignment using the command line (Tophat v1.3.3). I've uploaded the files (accepted.bam) onto Galaxy and upon doing the Cufflinks analysis on the Galaxy interface, it shows an error. I had to look at the options. I've change the options to default and run it again but still

[galaxy-user] Removal of duplicates and Cufflinks usage

2011-11-04 Thread Lizex Husselmann
Hi All I've started analyzing my RNA-Seq data for two time points: Day0 and Day4 for control and treated. I've done aligning the data to the reference genome using Tophat. I've removed duplicates from the data sets. Could somebody please tell me, how important is it to remove duplicates and