Re: [galaxy-user] Read count issue with clipper/collapser

2011-04-15 Thread kenlee nakasugi
I've pinpointed the cause to be a hyphen followed by a numeric in the fasta header: if the header is: ILLUMINA-08A740_:8:1:2219:1057#0/1 then the read counts will not be accurate in the collapsed output. After the 'ILLUMINA', if I replace the -08 with _08, or delete the numbers so that it

Re: [galaxy-user] Histogram interpretation

2011-04-15 Thread Moein Farshchian
Dear All I have combined H3K4me3 pattern in a specific region (Info: UCSC Main on Human: wgEncodeBroadHistoneGm12878CtcfStdPk (genome)) with RefSeq genes in that region (CSC Main on Human: refGene (genome)) and get this pdf histogram. I was wondering if someone help me on interpretation of it.

Re: [galaxy-user] Help!!!!!! with Galaxy Cloud!!!!!

2011-04-15 Thread Mike Dufault
Hello again, So I am able to see all of the .dat files in /mnt/galaxyData. What commands can I use to download a file to my HD? Also, what program should I use to open the .dat file? Thanks again, Mike --- On Wed, 4/13/11, Enis Afgan eaf...@emory.edu wrote: From: Enis Afgan eaf...@emory.edu

Re: [galaxy-user] Help!!!!!! with Galaxy Cloud!!!!!

2011-04-15 Thread Enis Afgan
Hi Mike, You should be able to download the desired file(s) directly from Galaxy by expanding the desired history item and then clicking the 'disk' (i.e., download) icon. Alternatively, if you want to copy the file by hand directly from the file system on the instance, the following is the

Re: [galaxy-user] Help!!!!!! with Galaxy Cloud!!!!!

2011-04-15 Thread Enis Afgan
I don't think you'll need to convert the file other than maybe renaming the extension (mv filename.dat filename.bam) because Galaxy just adds that same extension to each file while the metadata that it keeps tell it which format the file is in. Just try opening the file up using the tool you were

Re: [galaxy-user] Nucleotide analysis - GC percentage

2011-04-15 Thread Douglas Allan
please remove me from mailing list - thanks On 14-04-2011, at 9:26 AM, Guru Ananda wrote: Thanks for pointing this out, Brad. Both geecee and infoseq are in fact available on Galaxy under EMBOSS section. Guru. On Thu, Apr 14, 2011 at 12:13 PM, Brad Chapman chapm...@50mail.com wrote:

Re: [galaxy-user] unsubscribe

2011-04-15 Thread Yi Yin
I have the same trouble as Giovanni, too. The confirmation email for unsubscription never arrived. It would be very nice if someone could help remove my email from the subscription list. Thanks! On Fri, Apr 15, 2011 at 11:53 AM, nic blouin nblo...@mail.uri.edu wrote: I am having the same

[galaxy-user] database on the cloud

2011-04-15 Thread Randall, Thomas (NIH/NIEHS) [C]
I set up a galaxy instance on the cloud, but got the following error when trying to convert a bed file of hg18 coordinates to fasta sequence with the Extract Genomic DNA tool as a test of the functionality of this instance: No sequences are available for 'hg18', request them by reporting this

Re: [galaxy-user] unsubscribe

2011-04-15 Thread David C. Schwartz, Ph.D.
Agreed‹I'm also having the same issues! David Schwartz From: Yi Yin fourthnat...@gmail.com Date: Fri, 15 Apr 2011 12:00:06 -0400 To: nic blouin nblo...@mail.uri.edu Cc: galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] unsubscribe I have the same trouble as Giovanni, too. The

[galaxy-user] SAM to BAM conversion problem

2011-04-15 Thread Kelkar, Hemant
Hello Galaxy Support, I generated an alignment with a fastq groomed illumina dataset using the Map with BWA tool in galaxy with the C. elegans ce6 genome. Interestingly the results (when I click on the history name) say that the database used was ce7. When I try to use the SAM-to-BAM tool, I