[galaxy-user] New Galaxy User

2011-06-16 Thread gaurav thareja
Hi All, I am a new user of Galaxy. I am trying to configure galaxy on my local system. I took clone of repository(https://bitbucket.org/galaxy/galaxy-dist). But I have couple of issues while understanding code base: 1. Is there any help file explaining directory structure and what all

Re: [galaxy-user] New Galaxy User

2011-06-16 Thread Louise-Amélie Schmitt
Hi 1 - You might want to read the bitbucket wiki instead: https://bitbucket.org/galaxy/galaxy-central/wiki/Home There you can find all the information you will need to understand the configuration files, but don't expect a detailed description of the whole directory structure. The information

[galaxy-user] galaxy cluster setup

2011-06-16 Thread Robert Jackson
Does anyone have practical info on galaxy setup on a cluster? Robert C. Jackson Software Systems Specialist III The University of Texas Pan-American Computer Support Services Division of Information Technology Phone: 956-665-2455 Fax: 956-665-8777

[galaxy-user] SRMA on a local galaxy instance?

2011-06-16 Thread Gong, Henry
Does anyone know how to get SRMA to work properly in galaxy? I've used the pre-built 0.1.15 jar as well as a 0.1.16 jar I built from source, but the result of an SRMA re-alignment job in both instances is a python process which only takes a few percent CPU (or less, since it only seems to be

[galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Felix Mayr
Hi there, Looking at the output of the SplicingDiff files of CuffDiff, me and my colleagues are preplexed about the output of the p_values and q_values. We've tried different inputs of different samples to compare but never seem to manage to get p_values smaller than 0.50 and we keep getting

Re: [galaxy-user] galaxy cluster setup

2011-06-16 Thread Nate Coraor
Robert Jackson wrote: Does anyone have practical info on galaxy setup on a cluster? Hi Robert, Please see the production server documentation at: http://usegalaxy.org/production --nate Robert C. Jackson Software Systems Specialist III The University of Texas Pan-American Computer

Re: [galaxy-user] galaxy cluster setup

2011-06-16 Thread Roman Valls
Hello Robert, You may have a look at the official documentation on the wiki: https://bitbucket.org/galaxy/galaxy-central/wiki/Config/Cluster https://bitbucket.org/galaxy/galaxy-central/wiki/Config/ProductionServer You may also have a look at the slides from the past Galaxy Community conference:

Re: [galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Jeremy Goecks
Felix, You seem to be providing the correct inputs to Cuffdiff and it appears to be producing valid output. More information about setting parameter values and interpreting Cuffdiff can be found in manual: http://cufflinks.cbcb.umd.edu/manual.html#cuffdiff Good luck, J. On Jun 16, 2011, at

Re: [galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Rory Kirchner
What version of Cufflinks is your Galaxy installation running? A recent version (1.00 and 1.01) had a problem that was causing the splicing and promoter use tests to have very few differentially regulated genes, according to http://cufflinks.cbcb.umd.edu/. -rory On Jun 16, 2011, at 8:13 AM,

[galaxy-user] AXT and NIB files for AlignSeq.loc

2011-06-16 Thread John David Osborne
I was wondering how to get a hold of the axt and/or nib files required by alignseq.loc. It's not mentioned on the local NGA Galaxy setup page. I was surprised to learn that: Fetch Sequences - Extract Genomic DNA requires AXT or NIB alignment files in order to extract mouse genomic DNA from

[galaxy-user] aspera install on cloud install

2011-06-16 Thread Joseph Hargitai
Hi, anyone tried to install an aspera server for a galaxy cloud instance? The goal would be to feed some local data faster to the instance. best, joe ___ The Galaxy User list should be used for the discussion of Galaxy analysis and

[galaxy-user] Displaying MACS Peaks at UCSC

2011-06-16 Thread Steven Okino
Hello, I am new to Galaxy and am trying to analyze some ChIP-Seq data. I groomed illumina FASTQ data and used Bowtie to map it to hg18. I then used MACS to call ChIP-Seq peaks; over 13,000 were identified. Analysis of individual peaks shows good consistency with ENCODE data sets on UCSC, so I