Re: [galaxy-user] Changing Bowtie parameters in TopHat

2011-11-17 Thread Jeremy Goecks
Thanks for your help. I'm mapping reads from one organism to a related but different organism, so some of the parameters I'd like to adjust are to relax mapping stringency -specifically: -n 3 (allow 3 mismatches in seed) -e 250 (allow cummulative phred score of 250 [or some other value

Re: [galaxy-user] GATK

2011-11-17 Thread Jennifer Jackson
Hi Metge, Thank you for your kind comments! The GATK tool wrappers are still in an early beta stage of development. Because of this, we are not ready to support set-up in local instances yet. If you have functionality input for the version on our Test server, general feedback is welcomed on

[galaxy-user] Tools appearing without inputs in workflow editor

2011-11-17 Thread Paul-Michael Agapow
A peculiar bug, presented for your input: A colleague has built a few tools and is trying to incorporate them into a workflow. However: 1. When he creates an new empty workflow, edits it and adds the tools, they appear without inputs (i.e. the icons/boxes on the diagram have no input

Re: [galaxy-user] GATK

2011-11-17 Thread Daniel Blankenberg
Hi Franzi, You have one too many hg19 in there. The fields go like: unique_build_id dbkey display_name fasta_file_path valid_tool_suites so: hg19hg19hg19 /drive1/galaxy/reference/hg19/sam_index/hg19_ref.fa gatk But do note that these tool integrations are still

Re: [galaxy-user] Using Galaxy Cloudman for a workshop

2011-11-17 Thread Ravi Madduri
Clare I wonder if you would be open to share the details of your setup and what you had to do for this once you are done. I think it would be incredibly useful to community (at least it would be for me ;-)) Thanks! On Nov 17, 2011, at 5:14 PM, Clare Sloggett wrote: Hi Enis, Jeremy,

Re: [galaxy-user] Using Galaxy Cloudman for a workshop

2011-11-17 Thread Clare Sloggett
On Fri, Nov 18, 2011 at 12:56 AM, Jeremy Goecks jeremy.goe...@emory.edu wrote: Scalability issues are more likely to arise on the back end than the front end, so you'll want to ensure that you have enough compute nodes. BWA uses four nodes by default--Enis, does the cloud config change this