[galaxy-user] Creating a galaxy tool in R - You must not use 8-bit bytestrings

2012-04-24 Thread Dan Tenenbaum
Hello, I'm a galaxy newbie and running into several issues trying to adapt an R script to be a galaxy tool. I'm looking at the XY plotting tool for guidance (tools/plot/xy_plot.xml), but I decided not to embed my script in XML, but instead have it in a separate script file, that way I can still

Re: [galaxy-user] MegaBLAST output

2012-04-24 Thread Jennifer Jackson
Hi Sarah, We appreciate all of the information you have provided and have been working here since yesterday to investigate the issue in more detail. This includes incorporating the additional data both you and Peter have been posting. We don't have anything conclusive to report yet, but it

Re: [galaxy-user] MegaBLAST output

2012-04-24 Thread Peter Cock
On Tue, Apr 24, 2012 at 10:24 PM, Jennifer Jackson j...@bx.psu.edu wrote: ..., using the BLAST+ BLASTN megablast wrapper that Peter authored, in a local or cloud instance, would be the best immediate remedy (this version has the standard 12 column output). Sequence length data could always be

Re: [galaxy-user] How to replace ensembl gene ID with gene names in Cuffdiff output?

2012-04-24 Thread Jennifer Jackson
Hi Wei, BioMart has tools to extract tabular data that maps Ensembl transcript identifiers to alternate identifiers, gene symbols, etc. See the tool under Get Data - BioMart Central server. You'll likely have to map from Ensembl transcriptID - HGNC transcript - HGNC gene

Re: [galaxy-user] MegaBLAST output

2012-04-24 Thread Jennifer Jackson
Thanks Peter, Excellent point. From there, the Cut tool could be used to reorganize the output to exactly match that of the 13-column regular megablast output. So, no external data needed, no tool modifications needed. This can't be done on the main public Galaxy instance as BLAST+ is not

Re: [galaxy-user] Cuffdiff

2012-04-24 Thread Jennifer Jackson
Hi Ateequr, This post from today has information another member found at seqanswers.com, directly from the CuffLinks/Merge/Diff tool author: http://user.list.galaxyproject.org/Re-1-cuffcompare-or-cuffmerge-td4581029.html Best, Jen Galaxy team On 4/17/12 8:00 AM, Ateequr Rehman wrote: Dear