[galaxy-user] Dear Galaxy Developer, where is the history/workflow file stored in the local machine?

2012-03-19 Thread 杨晨
Dear Galaxy Developer,
where is the history/workflow file stored in the local machine?
I mean the real file store in the disk instead of browser access.


Thanks and Best regards


--

Yang Chen
Room 635, No.41 Building,320 Yueyang Road
Institute of Biochemistry and Cell Biology
Shanghai Institutes for Biological Sciences
Chinese Academy of Sciences
Shanghai,P.R.China
Post Code:200032
Tel: 86-21-54921375___
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[galaxy-user] Combining two workflows into one

2012-03-19 Thread Innocent Onsongo
Galaxy Team,

I would like to create a workflow by combining portions of two
different workflows. How do I do this in Galaxy?

Thanks,
Getiria Onsongo
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Re: [galaxy-user] Combining two workflows into one

2012-03-19 Thread Peter Cock
You forgot to CC the mailin list.

 On Mon, Mar 19, 2012 at 12:02 PM, Peter Cock p.j.a.c...@googlemail.com 
 wrote:
 On Mon, Mar 19, 2012 at 4:53 PM, Innocent Onsongo onson...@umn.edu wrote:
 Galaxy Team,

 I would like to create a workflow by combining portions of two
 different workflows. How do I do this in Galaxy?

 Thanks,
 Getiria Onsongo

 One outline solution is as follows:

 (1) Start with an empty history.
 (2) Upload/import the input files
 (3) Run first workflow
 (4) Run second workflow
 (5) Save history as a new combined workflow
 (6) Edit new workflow to remove any redundant steps

 This is of course limited in functionality compared to what
 might be possible in a future version of Galaxy.

 Peter

On Mon, Mar 19, 2012 at 5:05 PM, Innocent Onsongo onson...@umn.edu wrote:
 I was hoping I could avoid re-running the two workflows since they
 both take a relatively long time to run. Looks like I might have to do
 it after all.

Use smaller datasets? Ideally you would have a small test set
covering positive and negative example.

Or you can manually create the workflow in the workflow editor,
starting from one of the existing ones and adding the new tools
and connecting them together.

Peter
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Re: [galaxy-user] Combining two workflows into one

2012-03-19 Thread Innocent Onsongo
Thanks. I was hoping there was an easier way e.g., selecting the two
workflows and exporting them into a workflow canvas. The workflows are
not too big so manually creating the workflows will probably be the
faster option for now.

On Mon, Mar 19, 2012 at 12:09 PM, Peter Cock p.j.a.c...@googlemail.com wrote:
 You forgot to CC the mailin list.

 On Mon, Mar 19, 2012 at 12:02 PM, Peter Cock p.j.a.c...@googlemail.com 
 wrote:
 On Mon, Mar 19, 2012 at 4:53 PM, Innocent Onsongo onson...@umn.edu wrote:
 Galaxy Team,

 I would like to create a workflow by combining portions of two
 different workflows. How do I do this in Galaxy?

 Thanks,
 Getiria Onsongo

 One outline solution is as follows:

 (1) Start with an empty history.
 (2) Upload/import the input files
 (3) Run first workflow
 (4) Run second workflow
 (5) Save history as a new combined workflow
 (6) Edit new workflow to remove any redundant steps

 This is of course limited in functionality compared to what
 might be possible in a future version of Galaxy.

 Peter

 On Mon, Mar 19, 2012 at 5:05 PM, Innocent Onsongo onson...@umn.edu wrote:
 I was hoping I could avoid re-running the two workflows since they
 both take a relatively long time to run. Looks like I might have to do
 it after all.

 Use smaller datasets? Ideally you would have a small test set
 covering positive and negative example.

 Or you can manually create the workflow in the workflow editor,
 starting from one of the existing ones and adding the new tools
 and connecting them together.

 Peter



-- 
Getiria Onsongo, Ph.D.
Bioinformatics Research Scientist
Masonic Cancer Center,
University of Minnesota
Minneapolis, MN 55455
Phone: 612-625-0101
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[galaxy-user] Cuffdiff result P and q values

2012-03-19 Thread Ateequr Rehman
Dear galaxy user
After running cuffdiff on my two samples (SAM files from bowtie)
i got a list with p and q values, and löast colum is saying abou significance

with P value, it seems like the comparison should be significant, but in Q 
value is 1, and last coumn is saying not significant

any one have an idea, how to interpret it , should we take any comparsion with 
less than 0.05 p value as significant or not 


tables in excel looks like it

Nay help is welcome

best
Ateeq



test_id gene_id gene locus sample_1 sample_2 status value_1 value_2 
log2(fold_change) test_stat p_value q_value significant 
CUFF.428.1 CUFF.428 - gi|190572091|ref|NC_010943.1|:1575813-1577629 q1 q2 
NOTEST 171.773 605.136 -150.518 588.996 3,86E-05 1 no 
CUFF.462.1 CUFF.462 - gi|190572091|ref|NC_010943.1|:1680283-1681214 q1 q2 
NOTEST 696.628 322.149 -111.266 538.062 7,42E-03 1 no 
CUFF.635.1 CUFF.635 - gi|190572091|ref|NC_010943.1|:2343969-2346219 q1 q2 
NOTEST 396.469 223.951 -0.824027 476.902 1,85E-01 1 no 
CUFF.512.1 CUFF.512 - gi|190572091|ref|NC_010943.1|:1840464-1843486 q1 q2 
NOTEST 136.314 70.604 -0.949109 422.322 2,41E-01 1 no 
CUFF.632.1 CUFF.632 - gi|190572091|ref|NC_010943.1|:2346561-2347408 q1 q2 
NOTEST 351.508 167.567 -106.882 415.844 3,20E-01 1 no 
CUFF.941.1 CUFF.941 - gi|190572091|ref|NC_010943.1|:3664426-3665364 q1 q2 
NOTEST 282.247 133.798 -10.769 412.254 3,75E-01 1 no 
CUFF.616.1 CUFF.616 - gi|190572091|ref|NC_010943.1|:2301552-2303180 q1 q2 
NOTEST 169.682 744.885 -118.774 462.107 3,82E-01 1 no 
CUFF.617.1 CUFF.617 - gi|190572091|ref|NC_010943.1|:2295763-2297758 q1 q2 
NOTEST 225.933 112.178 -101.011 454.517 5,49E-01 1 no 
CUFF.9.1 CUFF.9 - gi|190572091|ref|NC_010943.1|:41597-42402 q1 q2 OK 1729.08 
2797.07 0.693913 -4.461 8,16E-01 0.000179474 yes 
CUFF.956.1 CUFF.956 - gi|190572091|ref|NC_010943.1|:3665445-3669232 q1 q2 
NOTEST 518.525 323.653 -0.679966 444.565 8,76E-01 1 no 
CUFF.549.1 CUFF.549 - gi|190572091|ref|NC_010943.1|:2043111-2043664 q1 q2 OK 
7148.23 11816.4 0.725138 -421.443 2,50E+00 0.000275446 yes 
CUFF.872.1 CUFF.872 - gi|190572091|ref|NC_010943.1|:3489557-3490326 q1 q2 
NOTEST 220.274 840.662 -13.897 416.179 3,16E+00 1 no 
CUFF.636.1 CUFF.636 - gi|190572091|ref|NC_010943.1|:2348784-2352394 q1 q2 
NOTEST 114.384 601.415 -0.927447 414.807 3,35E+00 1 no 
CUFF.605.1 CUFF.605 - gi|190572091|ref|NC_010943.1|:2271979-2275960 q1 q2 
NOTEST 217.007 133.837 -0.697264 409.373 4,24E+00 1 no 
CUFF.568.1 CUFF.568 - gi|190572091|ref|NC_010943.1|:2160538-2164415 q1 q2 
NOTEST 74.365 377.013 -0.980011 395.097 7,78E+00 1 no 
CUFF.597.1 CUFF.597 - gi|190572091|ref|NC_010943.1|:2250029-2250918 q1 q2 
NOTEST 229.389 105.246 -112.403 386.937 0.000109116 1 no 

 
Ateequr Rehman
House No. 2 ground floor
Blauenstr. 10
79115 Freiburg im Breisgau___
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