[galaxy-user] FTP upload problem
Hi everyone, When I tried to upload my files using Filezilla, I got the error message 530 Sorry, the maximum number of clients (3) for this user are already connected. Can anyone give me some suggestions how to solve this problem? I have been stuck here for a whole day. Thanks very much! Yan ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] help for alternative splicing with RNA-seq analysis
Hello Jianguang, The RNA-seq tutorial was just updated: http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise Hopefully this helps, Jen Galaxy team On 8/9/12 10:41 AM, Du, Jianguang wrote: I have RNA-seq datasets of several cell types. I want to compare alternative splicing events between diffrent cell types. Can anyone show me the protocol/workflow or direct me to the tutorial? Thanks. Jianguang ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ -- Jennifer Jackson http://galaxyproject.org ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] Streptococcus suis genome
Hello Jörg, We can add your genome to the list to be prioritized for inclusion, but the quickest way to use this data in Galaxy is with the custom genome option. Instructions are in our wiki (below), but there are really only two short steps: 1 - load the fasta record into your history 2 - select the reference genome from your history's list of fasta datasets (instead of the pull-down native builds list) http://wiki.g2.bx.psu.edu/Support#Custom_reference_genome Hopefully this helps to get your analysis going! Jen Galaxy team On 8/9/12 4:28 AM, Joerg Willenborg wrote: Dear Sir or Madam, with interest I followed up your Galaxy platform and I am planing a Bowtie mapping with RNA-Seq data. Unfortunately the reference genome I am looking for is not included in the drop down menu. Do you think it is possible to add the Streptococcus suis P1/7 (NC_012925.1) genome to the menu? I would appreciate any reply on this email and thank you very much in advance Kind regards Jörg Willenborg Joerg Willenborg, PhD Institute of Microbiology University of Veterinary Medicine Hannover Bischofsholer Damm 15 D-30173 Hannover Germany Tel: +49511-8567595 e-mail: joerg.willenb...@tiho-hannover.de ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ -- Jennifer Jackson http://galaxyproject.org ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] how to split paired-end dataset of FASTQ format
not sure but think that they need to be in fastqsanger format to be used in the splitter. You probably have to convert them first with the groomer. But since I am only finding out Galaxy myself, maybe wait for a more user with more experience to answer your question. bram Bram Van den Bergh Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, K.U.Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Phone: +32 16 32 16 31 (secretariat) Fax.: +32 16 32 19 63 www.cmpg.be http://www.biw.kuleuven.be/dtp/cmpg/spi Van: galaxy-user-boun...@lists.bx.psu.edu [galaxy-user-boun...@lists.bx.psu.edu] namens Du, Jianguang [jia...@iupui.edu] Verzonden: donderdag 9 augustus 2012 23:29 To: galaxy-user@lists.bx.psu.edu Onderwerp: [galaxy-user] how to split paired-end dataset of FASTQ format I downloaded RNA-seq dataset at FASTQ format from SRA of NCBI. I uploaded the dataset onto Galaxy. The dataset is paired-end. I want to split it into two datasets (one for each end) with FASTQ splitter. But the name of the dataset does not appear under FASTQ reads. How should I do to solve this problem? Thanks. Jianguang ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
