[galaxy-user] What is the quality score type for the Solid datasets downloaded from SRA of NCBI?
Hi all, Please help with the quality score type for the downloaded Solid datasets. I downloaded RNA-seq datasets, which were generated by AB Solid system, as base space and at FastQ format from SRA of NCBI. I uploaded the datasets onto the online sever Galaxy and change the datatype directly into fastqsanger and then test the quality by running FastQC. The output per base quality of solid dataset (please take look at the attached figure per_base_quality-Solid) is quite different from the output per base quality of Illumina dataset (please compare with the attached figure per base quality-Illumina). The top score for Solid dataset is about 31, however the top score for Illumina dataset is 38. What is the quality score type for the downloaded Solid datasets when downloaded as base space and at FastQ format from SRA of NCBI? Please help me solve this problem. Thanks. Best regards. Jianguang Du attachment: per_base_quality-Illumina.pngattachment: per_base_quality-Solid.png___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
[galaxy-user] Internal Server Error and slow to refresh History
Hi All, When I tried to download the filtered datasets from my History at Galaxy to my computer, I always got this error information: Internal Server Error Galaxy was unable to sucessfully complete your request An error occurred. This may be an intermittent problem due to load or other unpredictable factors, reloading the page may address the problem. The error has been logged to our team. If you want to contact us about this error, please reference the following GURU MEDITATION: #8aa15caba14d4a3e85ce7ab069 What is the cause for this problem? In addition, It takes about 3 to 5 minutes to refresh my Histories or to view different Histories. However, it is very quick to refresh Saved Histories. Is it normal? Thanks. Best, Jianguang ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
