Re: [galaxy-user] problem running Augustus

2013-10-16 Thread Bjoern Gruening
Hi Claudio,

I will have a look at it, at latest tomorrow, promised.

Cheers,
Bjoern (Augustus wrapper developer)

 
 
 I am trying to use the genome Annotation tool Augustus on my locally
 installed copy of Galaxy. After loading the data file, all analyses
 stop and deliver the following:
 
 
 error
 an error occurred with this dataset: /bin/sh:1:augustus: not found
 python: can't open file: '/extract_features.py': [Errno 2] No such
 file or directory
 
 
 Python is installed on my system (Ubuntu 12.04, Python 2.7.3)
 
 
 It must be something very basic I'm missing, but more basic are my
 linux skills…
 
 
 Thanks a lot
 
 
 Claudio
 
 
 
 ~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^
 Claudio Slamovits,
 Canadian Institute for Advanced Research
 Department of Biochemistry and Molecular Biology
 Dalhousie University
 5850 College Street, Halifax, Nova Scotia
 B3H 4R2, Canada
 
 Lab Webpage: http://slamo.biochem.dal.ca
 Lab: (902) 494 7894
 Office: (902) 494 8825
 Fax: (902) 494 1355
 ^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~
 
 
 Reality is what it is, not what you want it to be. 
 - Frank Zappa 
 
 
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Re: [galaxy-user] Fwd: About empty no peek problem

2013-09-03 Thread Bjoern Gruening
Hi Xiao,

the attached ones are working for me, hope for you too.

Cheers,
Bjoern

 Dear Bjorn
 
 
 The configure xml file and the relevant R scripts is attached. 
 
 
 Thank you for all your help.
 
 
 Regards,
 XiaoTao
 The University of HongKong
 
 
 2013/9/3 Björn Grüning bjoern.gruen...@pharmazie.uni-freiburg.de
 Hi Xiao,
 
 can you show us the complete wrapper XML script?
 
 Thanks,
 Bjoern
 
 
 
  -- Forwarded message --
  From: XiaoTao Jiang biofuture.ji...@gmail.com
  Date: 2013/9/2
  Subject: About empty no peek problem
  To: galaxy-user-requ...@lists.bx.psu.edu
 
 
  Dear all
 
 
  Today I embed one of my R script into local galaxy, I have
 encountered
  a strange problem.  The script can successfully in local.
 However,
  when I run it online in galaxy, the output file is empty and
 in
  history panel, it display empty and no peek.
 
 
  I searched the mailing list, but still can not find
 solutions to solve
  it.
 
 
  The command display in sever is : key part
 
 
  galaxy.jobs.runners.local DEBUG 2013-09-02 17:29:49,297
 executing: R
  CMD BATCH --slave
 
 --args 
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_44.dat
  
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_64.dat
  barchart.r
  galaxy.jobs.runners.local DEBUG 2013-09-02 17:29:49,493
 execution
  finished: R CMD BATCH --slave
 
 --args 
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_44.dat
  
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_64.dat
  barchart.r
  galaxy.jobs DEBUG 2013-09-02 17:29:49,538 Tool did not
 define exit
  code or stdio handling; checking stderr for success
  galaxy.tools DEBUG 2013-09-02 17:29:49,697 Error opening
 galaxy.json
  file: [Errno 2] No such file or directory:
 
 
 '/Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/job_working_directory/000/71/galaxy.json'
  galaxy.jobs DEBUG 2013-09-02 17:29:49,759 job 71 ended
 
 
  I check the generated output file
 
  
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_64.dat
 
 
  It is empty zero in size
 
 
  and then I run the command in local command
 
 
  R CMD BATCH --slave
 
 --args 
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_44.dat
  
 /Users/jiangxiaotao/Programe/galaxy-galaxy-dist-a4113cc1cb5e/database/files/000/dataset_64.dat
  barchart.r
 
 
 
  The output is correct.
 
 
 
 
  Any suggestions will be appreciated !!
 
 
  Xiaotao JIANG
  Regards,
  The University of HongKong
  Department of Civil Engineering
 
 
 
 
 
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argsall - commandArgs()
input - argsall[6]
output - argsall[7]

tabl - read.table(file=input, header=TRUE, sep=\t)


fac - levels(tabl[,2])

#fac
#length(fac)
ini - rep(0,length(fac))
#for (q in 1:length(fac)) {
#  ini[q] - 0
#}

#ini

for (i in 1:length(tabl[,2])) {  
for( j in 1:length(fac) ){
  if(tabl[,2][i] == fac[j]){
ini[j] - ini[j] + tabl[,3][i]
  } 
}
}

figureformat - fi.pdf
pdf(figureformat)
par(mar=c(5,12,4,2))
barplot(ini, names.arg=fac,col=red, width=0.2, axis.lty=0, las=2, 
xlab=Frequence of ARGs, main=Bar Plot of ARGs Class, cex.axis=1, 
cex=0.7,offset=0, font=2, adj=0, horiz=T,border=NA, density=NA)
dev.off()

file.copy(figureformat, output, 

Re: [galaxy-user] Galaxy Tool Version Question

2013-08-28 Thread Bjoern Gruening
Hi Scott,

please click on the 'i' info icon in one of your datasets (next to the
download button) you will find many additional information's, including
the wrapper version and the version of the underlying tool.

Cheers,
Bjoern

 To Whom it May Concern, 
 
 
 I was trying to find the version information regarding certain RNA-Seq
 analysis (Tophat, Tophat2, Cufflinks), and have had difficulty finding
 the versions for either Tophat instance.
 
 
 Is there a way to find out what version of Tophat is being used?
 
 
 Thank you for your time,
 Scott Nixon
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[galaxy-user] Join the HOMER Galaxy wrapper development!

2013-08-13 Thread Bjoern Gruening
Hi Galaxy Tool developers!

I have started to integrate the HOMER suite into Galaxy. My initial
attempt can be found here: 

https://github.com/bgruening/galaxytools/tree/master/homer

Currently, we make use of:
- Tool Shed definitions of all required 3rd Party Software
- datatype definitions
- loc files to define different HOMER datasets
- macros
- compostite datatypes (html output)

I think that project can be a good start to learn Galaxy Tool
development with all its simple and enhanced features. Also HOMER is a
complex suite and you can add small and really big features if you like.

If you are interested but did not have any experience in Tool
development, do not hesitate to ask. I will try to help and explain as
much as I can. 

For the beginning I included only 3 tools, and only one of them with a
bunch of parameters. So there are many things to do ...

If anyone is interested in leaning Galaxy Tool development and want to
contribute. Fell free to join and contact me.

Cheers,
Bjoern


P.S. I do not mind to merge my changes to the already existing
repository from Kevin, but I was not able to contact him. 
http://toolshed.g2.bx.psu.edu/view/kevyin/homer

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Re: [galaxy-user] Illumina Methylation tool in toolshed

2013-07-29 Thread Bjoern Gruening

Hi Sachit,

not in the toolshed but you can find a start here:

https://github.com/bgruening/galaxytools/tree/master/illumina_methylation_analyser

its for the 450k chips, I have also some tools for WGMA if
you are interested.

Cheers,
Bjoern

 Hi,
 
 This is my third post regarding this matter but however I
 didn't get any
 reply. I am trying to find a tool for r Illumina
 Methylation. Is there any
 available on the tool shed or test tool shed?
 
 Thanks,
 
 Sachit
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Re: [galaxy-user] Illumina Methylation tool in toolshed

2013-07-29 Thread Bjoern Gruening
Not that I know of, mine is wrapping IMA.

cheers,
Bjoern


 Hi Bjoern,
 
 Thank you for your reply.  To be precise I am looking for
 a wrapper of *
 lumi.

*http://www.bioconductor.org/packages/2.12/bioc/html/lumi.html
 
 Do we have that in main tool shed or test tool shed or in
 github?
 
 Thanks,
 
 Sachit
 
 
 On Mon, Jul 29, 2013 at 2:53 PM, Bjoern Gruening 
 bjoern.gruen...@pharmazie.uni-freiburg.de wrote:
 
 
  Hi Sachit,
 
  not in the toolshed but you can find a start here:
 
 
 

https://github.com/bgruening/galaxytools/tree/master/illumina_methylation_analyser
 
  its for the 450k chips, I have also some tools for WGMA
 if
  you are interested.
 
  Cheers,
  Bjoern
 
   Hi,
  
   This is my third post regarding this matter but
 however I
   didn't get any
   reply. I am trying to find a tool for r Illumina
   Methylation. Is there any
   available on the tool shed or test tool shed?
  
   Thanks,
  
   Sachit
 

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