Re: [galaxy-user] Quality report

2013-11-06 Thread Ido Tamir
Please be more specific about which quality of what gets reported. Why do you think its important to reduce the difference between mean and median? best, ido On Nov 6, 2013, at 4:50 PM, Benjamin Osei-agyeman benjy_o...@yahoo.co.uk wrote: Hi, After obtaining a quality report in galaxy I found

Re: [galaxy-user] problem with bowtie

2013-08-27 Thread Ido Tamir
You should add 10 lines from one of your files. Then its easier to understand the problem. best, ido On Aug 26, 2013, at 5:17 PM, Law, Michael J. la...@rowan.edu wrote: Hello, I am completely new to using galaxy. I have a quick question. I have uploaded my fastq files generated from my

Re: [galaxy-user] problem with bowtie

2013-08-27 Thread Ido Tamir
do I fix this? Thanks so much, Mike Michael Law la...@rowan.edu On Aug 27, 2013, at 4:44 AM, Ido Tamir wrote: You should add 10 lines from one of your files. Then its easier to understand the problem. best, ido On Aug 26, 2013, at 5:17 PM, Law, Michael J. la...@rowan.edu

Re: [galaxy-user] question

2013-07-28 Thread Ido Tamir
You could use the adaptor clip with e.g. a custom poly-A 'adaptor' its in FASTX-Toolkit for FASTQ data best, ido On Jul 28, 2013, at 8:44 PM, Larry Simpson larrys3...@gmail.com wrote: Hi Is it possible to trim a variable number of a specific nucleotide from the 3' ends of fastq RNA

Re: [galaxy-user] barcode splitter files web based

2013-07-10 Thread Ido Tamir
Is there now a better way to import files that are actually already in galaxy? Is there a trello card for this? It would save our users a lot of time and nerves to be able to do this. thank you very much, ido On Mar 21, 2013, at 6:01 AM, Jennifer Jackson j...@bx.psu.edu wrote: Hi Ann,

Re: [galaxy-user] Unmapped reads from tophat

2012-11-20 Thread Ido Tamir
On Nov 20, 2012, at 11:21 AM, Kevin Y wrote: Hello, Is there a way to get unmapped reads from tophat? Only the very latest tophat version 1.4.1 IIRC (and maybe tophat2 from the beginning) save unmapped reads into a fastq.gz file output, and its not an option. I don't think the galaxy output

Re: [galaxy-user] transformation fastq files

2012-01-12 Thread Ido Tamir
I think what you want is Collapse sequences in the Fasta manipulation tab. HTH, ido forgot the list CC. On Jan 11, 2012, at 5:36 PM, Florian Peschke wrote: Hi there, I am not quite sure if Galaxy can help me but I am looking for way to transform a fastq file into Unique Seuqence tags.

Re: [galaxy-user] Hi

2011-07-12 Thread Ido Tamir
On Jul 11, 2011, at 10:04 AM, YOGESH OSTWAL wrote: thanks a lot. Sorry for disturbing you again. Before starting with the actual data, can I try this analysis with already available IP and input files of datasets of illumina from NGS repository? why shouldn't you? select something from

Re: [galaxy-user] Hi

2011-07-11 Thread Ido Tamir
On Jul 10, 2011, at 8:00 AM, YOGESH OSTWAL wrote: Dear Galaxy users, This is Yogesh, a new galaxy user, very new to programming as well. Can anybody guide me from where to start to learn ChIP-Seq analysis? Maybe with galaxy you don't have to program. Its difficult to help you without

Re: [galaxy-user] program for filtering and trimming data

2011-06-10 Thread Ido Tamir
On Jun 10, 2011, at 7:10 PM, Joanne Rampersad wrote: Hi Is there an stand alone ( ie not a component of Bowtie etc ) program in galaxy that can filter or trim fastq data? its under NGS TOOLBOX BETA NGS: QC and manipulation best, ido