[galaxy-user] Quality report

2013-11-06 Thread Benjamin Osei-agyeman
Hi, After obtaining a quality report in galaxy I found out that there is a difference between the mean and median at each cycle. How can this difference be reduced using galaxy? Thanks in advance Benjy___ The Galaxy User list should be

Re: [galaxy-user] Quality report

2013-11-06 Thread Ido Tamir
Please be more specific about which quality of what gets reported. Why do you think its important to reduce the difference between mean and median? best, ido On Nov 6, 2013, at 4:50 PM, Benjamin Osei-agyeman benjy_o...@yahoo.co.uk wrote: Hi, After obtaining a quality report in galaxy I found

Re: [galaxy-user] Quality report

2013-11-06 Thread Benjamin Osei-agyeman
is there a difference between the mean and median in the fastqc report at each cycle? Benjy From: Benjamin Osei-agyeman benjy_o...@yahoo.co.uk; To: Ido Tamir ta...@imp.ac.at; Cc: galaxy-user@lists.bx.psu.edu; Subject: Re: [galaxy-user] Quality report Sent: Wed

Re: [galaxy-user] Quality report

2013-11-06 Thread James Taylor
On Wed, Nov 6, 2013 at 12:54 PM, Benjamin Osei-agyeman benjy_o...@yahoo.co.uk wrote: The report was obtained after running fastqc on my data. However after looking at the report statistics, the Quality is not good in the Per Base Sequence Quality and there is a difference between the mean