Re: [galaxy-user] question about using bowtie

2011-07-29 Thread Jennifer Jackson
Hello William, The tools in NGS: QC and manipulation, especially those in the sub-section AB-SOLiD data can do the manipulations needed before mapping. It may be helpful to view the screencast at http://usegalaxy.org, center pane, quickie #9. Hopefully this helps to get you started, Best,

[galaxy-user] question about using bowtie

2011-07-28 Thread William Light
I am trying to use bowtie to assign reads to the s. Cerevisiae genome. I have data from paired end SOLiD sequencing with two unique six base pair barcodes. Can I use bowtie to make csfasta and qual files from my mixed original data split by bar code? I know I can use the trim option to remove