Re: [galaxy-user] Cuffdiff-cummerbund with biological replicates problem

2013-07-31 Thread Jeremy Goecks
In the past, others have had success using Cummerbund with Galaxy, and there's even a Cummerbund wrapper in the tool shed: http://toolshed.g2.bx.psu.edu/view/jjohnson/cummerbund That said, it appears that replicate information is largely contained in the read group tracking files, which are

[galaxy-user] August 2013 Galaxy Update Newsletter is out

2013-07-31 Thread Dave Clements
Hello all, The August 2013 Galaxy Update is now availablehttp://wiki.galaxyproject.org/GalaxyUpdates/2013_08 . *Highlights:* - *GCC2013 Reporthttp://wiki.galaxyproject.org/GalaxyUpdates/2013_08#GCC2013_Report :* Meeting summaries, and links to videos, talks, posters, and Training

Re: [galaxy-user] Bowtie: Outputting unmapped reads

2013-07-31 Thread Mayank Tandon
That's a neat trick, and I definitely wouldn't have thought of that approach, so thanks for that! After I finished writing this out, I realized it was super long. So here are the questions I'm asking up front, so you can choose whether or not to read the details. Thanks! 1. How do I output the

Re: [galaxy-user] Bowtie: Outputting unmapped reads

2013-07-31 Thread Peter Cock
On Wednesday, July 31, 2013, Mayank Tandon wrote: That's a neat trick, and I definitely wouldn't have thought of that approach, so thanks for that! After I finished writing this out, I realized it was super long. So here are the questions I'm asking up front, so you can choose whether or

Re: [galaxy-user] Bowtie: Outputting unmapped reads

2013-07-31 Thread Mayank Tandon
Exactly. Jennifer's solution for outputting unmapped reads involves splitting the FASTQ file into basically two FASTA files, one with sequences and the other with the corresponding quality score string. So, yes, they would be matched files. On Wed, Jul 31, 2013 at 4:42 PM, Peter Cock