[galaxy-user] Maq consensus calling changes quality score of the read?

2012-03-14 Thread Antony Jose
Hi, We used the generate pileup tool with consensus base calling option using Maq. In the output, the quality scores of the bases were changed. For example if the input score of bases in the SAM file were 'IIJIH'. They were changed to '22321'. Is this a glitch or is this expected? Thank you. Antony

[galaxy-user] Maq consensus calling changes quality score of the read?

2012-03-14 Thread Antony Jose
Hi, We used the generate pileup tool with consensus base calling option using Maq with default options. In the output, the quality scores of the bases were changed. For example if the input score of bases in the SAM file were 'IIJIH'. They were changed to '22321'. Is this a glitch or is this expect

[galaxy-user] long wait on jobs

2012-05-18 Thread Antony Jose
Hi, I started a workflow yesterday (user name: antonymerlinj...@gmail.com) and it still hasn't gone past being queued to run. In fact, no jobs are running. Please advice. Thank you. Antony -- Antony M Jose, Dept. of Cell Biology & Molecular Genetics, University of Maryland, Rm 2116, Bioscience R

[galaxy-user] Bowtie and BWA queue..other jobs dont

2012-06-23 Thread Antony Jose
Hi, Bowtie and BWA jobs seem to be forever on queue, but other jobs run normally. Is there a problem or is this expected? Thank you. Antony -- Antony M Jose, Dept. of Cell Biology & Molecular Genetics, University of Maryland, Rm 2116, Bioscience Research Building, College Park, MD - 20742.

Re: [galaxy-user] Bowtie and BWA queue..other jobs dont

2012-06-25 Thread Antony Jose
has completed since this question was > originally sent in on Saturday. > > If your work is urgent, a cloud instance is the recommended alternative > for large NGS jobs: > http://getgalaxy.org/cloud > > Best, > Jen > Galaxy team > > > On 6/23/12 5:47 PM, Antony Jose

[galaxy-user] Gene BED to codon BED not working properly

2012-07-11 Thread Antony Jose
Hi, When using the gene BED to codon BED tool, I noticed that it is not accurately reporting the codons that make up a gene. For example, some of the codon are missing (particularly ones that span exon-exon junctions. Also, when changing reading frame from one exon to the next, the codons are not