[galaxy-user] Adding a reference genome

2011-06-30 Thread Jeremy Coate
how I would install it in my history? Thanks for your help. Sincerely, Jeremy Coate ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all repli

[galaxy-user] using files produced by "Barcode Splitter"

2011-07-18 Thread Jeremy Coate
I used the "Barcode Splitter" tool to split multiplexed RNA-Seq libraries into separate files. I would now like to map the reads from each of these fastq files to a reference genome. However, the fastq files generated by Barcode Splitter don't appear in the "Fastq File" pull-down menus within the t

Re: [galaxy-user] using files produced by "Barcode Splitter"

2011-07-18 Thread Jeremy Coate
** ** > > *From:* galaxy-user-boun...@lists.bx.psu.edu [mailto: > galaxy-user-boun...@lists.bx.psu.edu] *On Behalf Of *Jeremy Coate > *Sent:* Monday, July 18, 2011 1:44 PM > *To:* galaxy-user@lists.bx.psu.edu > *Subject:* [galaxy-user] using files produced by "Barcode Split

Re: [galaxy-user] using files produced by "Barcode Splitter"

2011-07-19 Thread Jeremy Coate
into the upload > tools URL textarea. > > Ideally, it would be nice for the tool developer to be able to include a > link in the html that would "promote" the file to a dataset in the current > history. > > JJ > > > On 7/19/11 3:08 PM, Jeremy Coate wr

[galaxy-user] Changing Bowtie parameters in TopHat

2011-11-16 Thread Jeremy Coate
My apologies if this has been covered before but I am using Galaxy Main and wonder if, when running TopHat, you can modify the mapping parameters used by Bowtie? It seems that the full parameter list for TopHat pertains only to the reads that aren't mapped by Bowtie (the reads spanning splice junct

Re: [galaxy-user] Changing Bowtie parameters in TopHat

2011-11-16 Thread Jeremy Coate
Hi Jeremy, Thanks for your help. I'm mapping reads from one organism to a related but different organism, so some of the parameters I'd like to adjust are to relax mapping stringency -specifically: -n 3 (allow 3 mismatches in seed) -e 250 (allow cummulative phred score of 250 [or some other value

Re: [galaxy-user] cuff... errors

2012-05-01 Thread Jeremy Coate
Hi all, FYI, I've gotten the same error recently when trying to run Bowtie (using Galaxy Main). Jeremy On Tue, May 1, 2012 at 4:37 PM, Richard Mark White wrote: > Hi, > Ive been trying to run cufflinks/cuffdiff but keep getting this error: Job > output not returned by PBS: the output datasets wer