[galaxy-user] Transcriptome Hypericum perforatum

To whom it may concern I would like to kindly ask you if you do have any experience in de-novo transcriptomic analysis (no reference genome available) who might give us some advice. Our main question is how to create the best set of cDNA contigs, on which we can map our RNAseq reads for the a

[galaxy-user] tophat issues

To whom it may concern I do have a problem with tophat. I can easily put fastq data to "history" and according to RNA-seq Analysis Exercise provided by Jeremy. We checked the type of Ascii ofset for the quality estimation. I tried even "quality data converter" set to 33 (we do have data of thi