Thank you so much, Jennifer!
Your method is pretty good for filtering the sequences that have A
at the 10th base position.But I'm sure if it have employed 10 bp
complementary criterion? For the antisense, the 5' will be T (in
complementary to A). Besides, my library contains other small
Hi Zhiqiang,
The regular expressions I shared are just filters. It strictly either
finds sequences with an A at base position 10 (first expression) or
without an A at base position 10 (second expression). All starting left
- right along the sequence text (or string). This may or may not be
Hi, Galaxy users!
I have a question on how to find out sense and antisense sequence.
I've got RNA seq data in the fastq format. The sequences inside are
partially complementary to each other (complementary is 10nt, while
entire is about 30nt). How can I separate these sequences into two
On Mon, Nov 26, 2012 at 6:47 PM, Zhiqiang Shu z...@bio.fsu.edu wrote:
Hi, Galaxy users!
I have a question on how to find out sense and antisense sequence. I've got
RNA seq data in the fastq format. The sequences inside are partially
complementary to each other (complementary is 10nt, while
Thank you, Peter! I think I should put more detailed information here.
What I'm doing is piRNA data. Two groups of piRNA (named sense and
antisense)are in the library. As I said, they are complementary to
each other for about 10 nt, while the whole length is about 30nt. For
the sense
Message-
From: galaxy-user-boun...@lists.bx.psu.edu
[mailto:galaxy-user-boun...@lists.bx.psu.edu] On Behalf Of Zhiqiang Shu
Sent: Monday, November 26, 2012 3:29 PM
To: Peter Cock
Cc: Galaxy-user
Subject: Re: [galaxy-user] Find out sense and antisense sequences
Thank you, Peter! I think I
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