Re: [galaxy-user] RNA-seq analysis withtout reference genome

2012-10-08 Thread Jennifer Jackson
Hello Alicia, There are two tools in the main Galaxy Tool Shed (http://toolshed.g2.bx.psu.edu/) that would likely be helpful. Read the input requirements for each to decide which is a better fit. Search for 'deseq' to find them: deseq_and_sam2counts deseq_hts To use these tools, a local

[galaxy-user] RNA-seq analysis withtout reference genome

2012-10-05 Thread Alicia R . PĂ©rez-Porro
Hi all, I'm about to start doing gene expression analysis but i don't have a reference genome. I got my sequences from Illumina and i did my assemblies with CLC bio. I was planning to use DESeq for the analysis but for that i need my gene expression count values. There is any tool in Galaxy that i