Re: [galaxy-user] finding open reading frames and corresponding genes

Hello, Making the assumption that your data is DNA (and not RNA), the tools under "NGS: SAM Tools" and "Operate on Genomic Intervals" can generate coordinates of the mapped reads which then can be correlated with known genes/ORFs from your bacterial genome (or related genomes, if you can obta

[galaxy-user] finding open reading frames and corresponding genes

Hi I am sequencing a bacterial genome and have assembled my Illumina reads (40 bp single) using bowtie with a reference genome. This generated a sam file. I would like to obtain a listing of the open reading frames from the bacterial genome and the corresponding genes that they are most similar to.