Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-03-02 Thread Jeremy Goecks
(cc'ing galaxy-user) Sorry Jeremy, I'm being a bit dense here - where exactly are these tools in > the tool list - I can't find them... David, Both tools are located within the 'Operate on Genomic Intervals' menu. Also, remember that tool search (Options -> Show Tool Search) is useful for fin

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-03-01 Thread David Matthews
;> >> --- On Tue, 3/1/11, Ying Zhang wrote: >> >> >> From: Ying Zhang >> Subject: Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID >> To: "David Matthews" >> Cc: galaxy-u...@bx.psu.edu >> Date: Tuesday, March 1, 20

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-03-01 Thread Ying Zhang
find out the gene_ID correspond to CUFF ID To: "David Matthews" Cc: galaxy-u...@bx.psu.edu Date: Tuesday, March 1, 2011, 10:59 AM Dear David: I followed your advices and downloaded reference sequence  from Emsemble, then I uploaded this file into galaxy, and then I run the cufflinks

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-03-01 Thread vasu punj
I believe you need to format the Ensemble file Chromosome columns is not correct.   Vasu --- On Tue, 3/1/11, Ying Zhang wrote: From: Ying Zhang Subject: Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID To: "David Matthews" Cc: galaxy-u...@bx.psu.edu Date: Tuesda

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-03-01 Thread Ying Zhang
Dear David: I followed your advices and downloaded reference sequence from Emsemble, then I uploaded this file into galaxy, and then I run the cufflinks using the file as a reference annotation, however I got error when I am running, the following the error message gave to me: An error occur

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-03-01 Thread David Matthews
Hi, Yeah, thats a good idea too!! I did not know about that tool, shows what I know (!) - thanks for the info! Cheers David On 1 Mar 2011, at 04:51, Jeremy Goecks wrote: > Ying, you could also try using the tools 'Fetch closest non-overlapping > feature' and 'Intersect' to find genes nearby

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-02-28 Thread Jeremy Goecks
Ying, you could also try using the tools 'Fetch closest non-overlapping feature' and 'Intersect' to find genes nearby transcripts/genes/TSSes of interest; for both tools, you'll want a reference annotation, either from UCSC or Ensembl. Best, J. On Feb 28, 2011, at 6:10 PM, David Matthews wrote

Re: [galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-02-28 Thread David Matthews
Hi, You need to supply a gene annotation file with cufflink to easily get the gene-id information. Without it, cufflinks simply tries its best to figure out what genes are present. The ensemble gtf file is quite a comprehensive one - there is a link to it on the cufflinks manual page. Good luc

[galaxy-user] how to find out the gene_ID correspond to CUFF ID

2011-02-28 Thread Ying Zhang
Dear Everyone: I have got one output file after I run Cufflink which contain gene expression information. However, I found out for each gene_ID, it has the format like, CUFF.1151175, do you have idea of how to find out the offical gene ID correspond to this CUFF ID? Thank you very much! Best Yin