Re: [galaxy-user] wig to bigwig error

2012-04-16 Thread Jennifer Jackson
Hi Mike, Yes, to get the .wig file out of the data, select the first "892177" lines. (Selecting "892178" would include the second track line, which you don't want). After looking one more time, not all data appears to be .wig. This is a multiple track group file, labeled as .wig, but the sec

Re: [galaxy-user] wig to bigwig error

2012-04-16 Thread Michael Sikes
Jen, A couple of uninformed questions. I gather from your response that the author lab submitted a multiple track group .wig file instead of a single track group .wig file, and that I need to generate a single track group file before the bigwig conversion will work. So, with regard to the

Re: [galaxy-user] wig to bigwig error

2012-04-16 Thread Jennifer Jackson
Hi Mike, I apologize if I wasn't clear, but the 'Select' was to show you how to identify the multi-track group wig files. I wanted to give you a way to screen similar files going forward. The wig-to-bigWig program in Galaxy comes from UCSC. It accepts .wig files with a single track group as

Re: [galaxy-user] wig to bigwig error

2012-04-16 Thread Michael Sikes
Jennifer, Thanks for your help. I ran the filter and sort tool as advised, and then ran the wig to bigwig on the new history item generated by the filter. This time I got a different error: 84: Wig-to-bigWig on data 83 0 bytes An error occurred running this job:stdin is empty of data Error

Re: [galaxy-user] wig to bigwig error

2012-04-13 Thread Jennifer Jackson
Hi Michael, This particular .wig file has a data format problem that is the root cause of the conversion error. Specifically, there is an extra track line in the file. This can be found using unix tools with a grep or in Galaxy with the tool "Filter and Sort -> Select" by matching the pattern

[galaxy-user] wig to bigwig error

2012-04-13 Thread Michael Sikes
Hi, I have hit a brick wall when trying to convert wig files from the GEO to bigwig files. Each time I try (and I have tried many times since October), I get the same error. For example, here is a downloaded wig file, that I assigned to the mouse mm8 genome, and the error I got when I tr