Re: [galaxy-user] Converting transcriptomes to proteomes

2011-06-10 Thread Pablo Cingolani
Hi David and John, If you send me your transcriptome data (I assume it's a GFF file), I can see if I can create a database from it. The version on galaxy shed is quite old. For this will have to use the latest development version, since there were many improvements on GFF parsing. I'm out of

Re: [galaxy-user] Converting transcriptomes to proteomes

2011-06-09 Thread David Matthews
Hi John (and Pablo), Thanks for the ideas. I assume this is similar to the "aaChanges" tool in the standard galaxy setup which I am working with. If I have no luck with that I'll look into the SNPEff tool - is it in the Galaxy toolshed? I think that, assuming most of the snps are previously cha

Re: [galaxy-user] Converting transcriptomes to proteomes

2011-06-09 Thread John David Osborne
Hi David, I've successfully used SNPEff (which can fit into galaxy) to make SNP effect predictions that would effect the proteome, but from genomic not transcriptomic data but I think it might still work on that... It takes in pileup/vcf format and predicts coding changes, upstream changes, sp