Re: [galaxy-user] SAM to GFF ?

2011-02-18 Thread Hans-Rudolf Hotz
Hi Felix It might be a little bit unusual to use the "generic feature format" (GFF) to show where your reads mapbut strictly speaking mapped reads on a genome are 'features' as well. You can do it the following way: first use the "Convert SAM to interval" tool (NGS: SAM Tools), and use

Re: [galaxy-user] SAM to GFF ?

2011-02-18 Thread vasu punj
I have used IGV with the Bam file and one ha sto generate index file which can be done in IGV, it work well. Gbrowser should also be OK. I think new version of IGV provide lot more features than previous one Vasu. --- On Fri, 2/18/11, Peter wrote: From: Peter Subject: Re: [galaxy-user] SAM

Re: [galaxy-user] SAM to GFF ?

2011-02-18 Thread Peter
On Fri, Feb 18, 2011 at 12:35 PM, Felix Hammer wrote: > > Hi Peter, > thx for the reply. > You're right I want to display mapped RNA seq data in gBrowse. > Currently I have the data in SAM format. > Maybe I can figure out a trick using the text manipulation tools. > thx, > Felix You forgot to CC

Re: [galaxy-user] SAM to GFF ?

2011-02-18 Thread Peter
On Fri, Feb 18, 2011 at 12:08 PM, Felix Hammer wrote: > Hi, > is there an easy way to use Galaxy to convert SAM to GFF? > thx, > Felix How so? SAM/BAM shows you where your reads map, GFF is used for annotation (e.g. where the genes are and what they may do). Are you talking about using SAM/BAM