AW: [Histonet] Fwd: SOP on embedding tissues of different sizes in same mold

2014-07-31 Thread Gudrun Lang
We avoid to put specimens of different size into one cassette in general (especially with biopsies). If I have too pieces of different size with a clear cut side, I put them both on the ground of the mold. I think one could never precisly put a specimen in a certain level into the mold, if it

AW: [Histonet] Fwd: Embedding skin

2014-07-31 Thread Gudrun Lang
As a first information, we use sliding microtomes with the knife set in an angel (called declination). We embed straight, because areas of same concistancy should be positioned in one line and in the cutting direction. Areas of more rigidity compress the smoother ones, if they are shifted in an

Re: [Histonet] Schiff : room temp or not

2014-07-31 Thread Rene J Buesa
All chemical reactions (= all histochemistry procedures) are governed by the Van't Hoff law that states that every 10ºC the speed of the reaction doubles (if it is +10ºC) or is reduced in half (if it is -10ºC) so, if you use the Schiff reagent (that has to be stored at 6-8 ºC) directly, the

Re: [Histonet] Schiff : room temp or not

2014-07-31 Thread Rene J Buesa
 This is another example of the ignorance level of some Joint Commission experts that really do not know what they are talking about. The Schiff reagent (the hay yellow solution of Basic Fucsin) is temperature sensitive and HAS to be stored refrigerated or it will lose its reactivity in just a

Re: [Histonet] Schiff : room temp or not

2014-07-31 Thread Grantham, Andrea L - (algranth)
Not ALL Schiff's NEED to be stored in the refrigerator. I use a Schiff's from Newcomer and it says to store at 15-30C (59-86F). It comes with a long shelf life and stains beautifully and can be reused a couple of times all at room temp. So why would I want to put this in the refrigerator?

[Histonet] Cassette Printer

2014-07-31 Thread Michael Ann Jones
Has anyone out there used the Primera automated cassette printer? Pros? Cons? We are close to the General Data model, however, this Primera looks kinda cool. Thank you for input, Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511

رد: Re: [Histonet] Schiff : room temp or not

2014-07-31 Thread j.rowaihi
I am totally agree with you Rene  I also experienced the same problem with CAP regarding Schiff's reagent storing. Regards Jamal Rowaihi Laboratory supervisor Al Borg Medical Laboratories *Sent from my cell phoner* الرسالة الأصلية من: Rene J Buesa rjbu...@yahoo.com التاريخ:

[Histonet] RE: Cassette Printer

2014-07-31 Thread Bea DeBrosse-Serra
Yes, we have one. Our first one was a lemon, but was replaced at no cost. Ever since then we had no problems whatsoever with it. We like it, because it has a small foot print and the printing is very clear, even with bar codes. The cons might be, that you have to unload the slides after a while

RE: Re: [Histonet] Schiff : room temp or not

2014-07-31 Thread Sanders, Jeanine (CDC/OID/NCEZID)
We purchase ours from Newcomer and it is stable at room temp. I check it periodically and have never had it go weak before the expiration date. That is one of the reasons I began using it was it was always ready to use. Newcomer does note that it is sensitive to humidity and to keep tightly

[Histonet] Helicobacter control tissue request

2014-07-31 Thread Preiszner, Johanna
Hi, could anyone send us some Helicobacter pylori control tissue? The commercial slides are disappointing for us...they cost a lot and not every slide has the bacteria. We are a small lab and just started doing GI..I just need a small piece of tissue until we have a positive specimen to use for

Re: [Histonet] RE: Noise reduction

2014-07-31 Thread Jay Lundgren
I don't know if it's your PA not being able to concentrate, or your transcriptionists complaining about noise on the recording, but have you considered solving the problem on the other end? Noise canceling headphones and/or a throat microphone for your PA. A real throat mic, (not a video game

[Histonet] RE: Histonet Digest, Vol 128, Issue 30

2014-07-31 Thread Hayden, Rebecca
I have recently started to take over the control blocks for our lab department. I want to use/make a form that I can show how many blocks I have approved for each stain and which blocks are in use. I have looked a little online to see if there is a form out there that already exists, but can

[Histonet] Gyn requisition

2014-07-31 Thread Melissa Burns
I was wondering if anyone is willing to share a copy of your gyn or gyn oncology based requisitions? Trying to create one and was hoping for some helping getting started. Email or fax to 8433998932 if you're willing to help. Thanks in advance! Melissa Sent from my iPhone

Re: [Histonet] RE: Histonet Digest, Vol 128, Issue 30

2014-07-31 Thread Michael Ann Jones
Rebecca, We use slide tray boxes with large index cards. We have first control slide - to prove block works. Then pre-cut control slides then one stained last control slide - to make sure entity is still there. And the block that is in use is stored in box with the slides. Index card notes