Re: [Histonet] Shrinkage

I find the same as Caroline Miller, about 20-25% with formalin fixation and routine processing. C On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet < histonet@lists.utsouthwestern.edu> wrote: > hi All, I have done some experiments in this area for mouse brains, and I > find that

[Histonet] BCL-10 for IHC-P

Has anyone been able to optimize an antibody to BCL-10 using Ventana's Ultraview detection? Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to

Re: [Histonet] Shrinkage

hi All, I have done some experiments in this area for mouse brains, and I find that there is actually an expansion of tissue after formalin fixation (around 10%), but then certainly a shrinkage to 100% dehydration agent of about 20% from the original size. We found similar results with alcohol,

Re: [Histonet] Shrinkage

LOL...Shrinkage...heh, heh. But seriously, there should be little to no gross shrinkage from formalin fixation and if the specimen is properly fixed, then there should be very little gross shrinkage as it is dehydrated. That is supposed to be the point! If someone is getting 30% shrinkage,

Re: [Histonet] formalin and shrinkage

My question refers to the difference in dimensions of the native tissue and the fixed tissue. – so without any influence of ethanol and xylol. I have no access to the whole publication you mentioned. If I understand the summary correctly, 2,5% shrinkage was found after formalinfixation.

Re: [Histonet] Davidson's fixative and IHC question

I think fixing with Davidson is ethanol-fixation in the first line. There are some antigens, that are susceptible to ethanol. Gudrun -Ursprüngliche Nachricht- Von: Judi Ford via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Montag, 22. Februar 2016 18:45 An:

Re: [Histonet] Davidson's fixative and IHC question

Today I learned what Davidson's fixative is! Thanks! Anyway, so it's a strong alcoholic formalin. The answer to your question is; yes. You need to be careful of overfixation, thus masking the epitope in question. From Dako: " Of the many pre-analytical variables which affect IHC and ISH

Re: [Histonet] formalin and shrinkage

Agree with you Jay Lundgren. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 2/22/16, 12:30 PM, "Jay Lundgren via Histonet" wrote: >I was taught at AFIP to expect

Re: [Histonet] formalin and shrinkage

I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose

[Histonet] Davidson's fixative and IHC question

Hi Everyone. Hope you all had a really good weekend. Thanks for the replies to my double ihc question. I do have another question; this one is from a friend of mine. Her client wants to do ihc on rabbit eye tissue. The tissue will be fixed in Davidson's fixative for 24 hours then in 10% NBF.

[Histonet] formalin and shrinkage

Hi! Today someone asked me about shrinkage caused by the fixation with formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30% percent. In my opinion shrinkage is mainly caused by the processing with dehydration and defatting. Formaldehyde renders the tissue harder but not

[Histonet] Georgia Society for Histology Annual Histopalooza

Spring is just around the corner and GSH cordially invites you to our annual meeting at the Lanier Islands resort. The Lakefront Lodge is 12 miles from the Mall of Georgia and offers many amenities, such as free wifi and heated outdoor pool. There's no better way to earn your CE credits. It's