[Histonet] plastic sectioning advice requested

2016-07-16 Thread daniel blackburn via Histonet
My lab hopes to get into plastic sectioning. We need to be able to process tissue pieces as large and thick as possible, but see that the largest embedding molds for JB4 are only 13x19mm by 5mm deep. We have two questions: (1) Do any of the available media (plastic or resins) allow one to

Re: [Histonet] dye in processor

2016-07-16 Thread Bob Richmond via Histonet
Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?" Safranin O - the solution your microbiology

Re: [Histonet] Histonet Digest, Vol 152, Issue 13

2016-07-16 Thread Bob Richmond via Histonet
Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?" Some time ago I worked in a lab that used