[Histonet] Movat's pentachrome

Thanks Bob. Somebody else mentioned her name also so I'll be sure to reach out to her. THE expert on the Movat pentachrome stain is Akemi Allison, a very senior histotechnologist who's published about it. I can't remember if she's on Histonet, but you can contact her on Facebook - I hear from

[Histonet] Movat's pentachrome

Hi Terri, I have used Poly Scientific kits in the past and agree that they are fabulous. If I were still in anatomic pathology I would wholeheartedly agree with you. However, I'm now in a research facility and the kit is simply cost prohibitive. Fortunately, I do now have time for homemade (a

Re: [Histonet] Modified Pentachrome

Thanks for sharing. It's very helpful. Where do you purchase your safran du gatinais/saffron from? At this point I'm ready to start growing my own Crocus sativus. On Thu, Sep 1, 2016 at 12:35 PM, Amos Brooks wrote: > Hi, > I use Lugol's Iodine all the time for this.

[Histonet] Modified Pentachrome

Hi, I use Lugol's Iodine all the time for this. It works just fine. I do purchase certain chemicals from manufacturers like Lugol's Iodine which I get from EMS. Most of the chemicals I make up myself from powder though. I have shared the procedure with you via Google Docs. I hope it helps.

[Histonet] H. pylori stain

Hello histoland We currently use Ventana's h. pylori antibody on our Benchmark ultra. Over the last few months our pathologists have been noticing "junk" that is staining with this antibody. It seems to be on the tissue more than in the tissue and its not on all slides. WE have tried

Re: [Histonet] (no subject)

We ran into this situation with TJC. We had a very limited check off sheet. In effort to standardize processes across the laboratory we adopted a program very similar to the NY facility whose program is referenced in the TJC leading practice library. It is very rigorous and time consuming

[Histonet] Histotech competency evaluation RE: (no subject)

Nirmala, Histotechs are not required to have competency assessment under CLIA regulations because, as you note, they do not interpret any tests or report any results. However, if they do grossing then they do require high complexity competency assessment. Having said that, if there is some

Re: [Histonet] Modified Movat's Pentachrome stain

Angela Lamberth at the La Jolla [California] Institute for Allergy & Immunology asks: >>I'm gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I've found a protocol used by the Children?s Hospital of Philadelphia Pathology

Re: [Histonet] (no subject)

Nirmala, Histotechs are not required to have competency assessment under CLIA regulations because, as you note, they do not interpret any tests or report any results. However, if they do grossing then they do require high complexity competency assessment. Having said that, if there is some

Re: [Histonet] Movat Pentachrome

If you are not that concerned over the alcian blue portion of the movats you can perform an elastic trichrome - that's what we do here. Mordant like you would in Bouins, and rather than stain with weigerts hemastoxylin, stain with the elastic portion of the VVG, differentiate like you would

Re: [Histonet] Movat's pentachrome

Just wondering why you are compelled to make all the reagents in house for the Movat's stain. I have used the kit k042 from Poly Scientic with fabulous results (and no, I have no gain from recommending them). Their solutions are wonderfully labeled and can be replenished individually, or by

[Histonet] (no subject)

Hi, What is everyone doing about the Histology Staff competencies. I know CLIA has several criteria for the med-tech competencies. Since the Histotechs do not report out results, how is everyone implementing this process. Any information is greatly appreciated. Nirmala Holy Name

[Histonet] Position Available Santa Barbara California Area

29/Aug/16 Requisition: 2067718 Job Title: Technical Support Specialist Description: Agilent inspires and supports discoveries that advance the quality of life. We provide life science, diagnostic and applied market laboratories worldwide with instruments, services, consumables, applications and

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background

Re: [Histonet] Movat Pentachrome

Angela, the Movat is a wonderful thing for showing loss of elastic fibers in emphysema and cardiovascular cases. We ran a ton of them in training because our lab/histo school was affiliated with the Texas Heart Institute. Currently at the hospital I work at we use a Kit(Polyscientific) for the

[Histonet] FW: NKX 3.1 protocol-

Hello Histonetters Was wondering if anyone would share their protocol for the above ABY we are utilizing the Ventana Ultra and purchased the ABY 3rd party from Cell Marque I would appreciate the help the insert doesn't have the standard protocol on it like the rest :( just want to be safe.

Re: [Histonet] Modified Movat's Pentachrome stain

Thank you for the suggestion. I'll ask around when I reconnect with people at Long Beach this month. On Thu, Sep 1, 2016 at 7:47 AM, Rene J Buesa wrote: > Do you have any contacts at any old histology lab, i.e., one that has been > in operation for more than 50 years? You may

Re: [Histonet] Modified Movat's Pentachrome stain

Do you have any contacts at any old histology lab, i.e., one that has been in operation for more than 50 years? You may find there iodine crystals and ask for a few grams. I used to have a 500 g bottle at my lab (which began in 1947).René On Thursday, September 1, 2016 10:39 AM, Angela

Re: [Histonet] Modified Movat's Pentachrome stain

I would prefer to not substitute anything yet the procedure calls for a minimum of 2 grams of iodine which I simply cannot buy in the United States thanks to new DEA regulations. On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa wrote: > Once you start substituting things in an

Re: [Histonet] Modified Movat's Pentachrome stain

Once you start substituting things in an original recipe, the outcome cannot be expected to be what the original recipe was supposed to deliver.Iodine crystals cannot be substituted by Lugol because, besides the iodine also contains its salts. and alcohol. They are two completely different