Re: [Histonet] Mouse bladder

2017-12-19 Thread Colleen Forster via Histonet
Exactly...and then it wont shrivel up C On Tue, Dec 19, 2017 at 3:09 PM, dusko trajkovic wrote: > I agree with Colleen, processing is the issue. You need to process on a > longer program in order to dehydrate and clear the agar properly. > dusko > > > On Tuesday,

Re: [Histonet] Mouse bladder

2017-12-19 Thread dusko trajkovic via Histonet
I agree with Colleen, processing is the issue. You need to process on a longer program in order to dehydrate and clear the agar properly.dusko On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet wrote: Be sure you are processing them

[Histonet] tissue blocks

2017-12-19 Thread Mica Fulgham via Histonet
Does anyone have any breast tissue or skin FFPE blocks that would be willing to send me? 5 blocks of each would be greatly appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Mouse bladder

2017-12-19 Thread Colleen Forster via Histonet
Be sure you are processing them on an overnight process. Even if the samples are small it takes that time to process the agar properly. I use Histogel and learned this lesson the hard way. My samples always "shriveled" up too until someone enlightened me about the processing time. I would think

Re: [Histonet] Mouse bladder

2017-12-19 Thread Liz Chlipala via Histonet
I would try histogel instead of agar. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com

[Histonet] Mouse bladder

2017-12-19 Thread Heather Marlatt via Histonet
Does anyone have a good protocol for mouse bladder embedded in agar that they are willing to share? We have mouse bladders in 2% agar and have tried a few different protocol variations from our usual mouse but the agar keeps shriveling up. Thanks Heather

[Histonet] PCR sectioning

2017-12-19 Thread Sanders, Jeanine (CDC/OID/NCEZID) via Histonet
Good morning! Do any of you out there section for PCR? If so, do you have a preference in microtomes? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329