Re: [Histonet] Histonet Digest, Vol 171, Issue 15

2018-02-16 Thread Diane Mahovlic via Histonet
I use methyl acetate to de-plasticize my PMMA slides.. . 30 minutes, air dry for a minute or two then re-hydrate in 100% and 95% alcohol, rinse in water. I make my own PMMA ,rather than using Technovit, maybe that has something to do with it. Has worked for me for 25+ years! Diane Diane Mahovli

Re: [Histonet] Histonet Digest, Vol 171, Issue 15

2018-02-16 Thread Madeleine Huey via Histonet
Subject: [Histonet] P16 Antibody Jason, I also tried many different vendors & clones many years ago (human FFPE tissues), and the winner is from Roche/Ventana (CINtec, Cat. 9517 = 11.5ml). You can get 5ml dispenser as well. *Madeleine Huey**, HTL & QIHC (ASCP)* Supervisor - Pathology (Histolog

[Histonet] used cryostats

2018-02-16 Thread Cynthia McGrady via Histonet
Hi. Our lab is in the market for a used or refurbished cryostat. Any tips or lead would be greatly appreciated. Thank.. Cindy McGrady, Hurley Medical Center, Flint, Michigan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsou

[Histonet] P16 Antibody

2018-02-16 Thread Jason McGough via Histonet
We are looking to bring in P16 to do in house but are having a hard time getting the stain to be clean. We are having issues with positive staining on tissue that should be negative. We are working with a concentrated antibody that we have diluted out to 1:6000 (which is 6 times the amount sugge

Re: [Histonet] [EXTERNAL] Deplastifying MMA

2018-02-16 Thread Dorothy Hu via Histonet
Hi Lori, Would you please tell me which company and catalog # of tetrahydrofuran you used before? For how many minutes you did for deplastifying in tetrahydrofuran? Thanks in advance. Dorothy Message: 3 Date: Wed, 14 Feb 2018 20:05:16 + From: "Garcia, Lori, M.Sc." To: "reuel.corne...@tsrh.

Re: [Histonet] Deplastifying MMA

2018-02-16 Thread Kusters, Ronald via Histonet
Dear Reuel, Maybe the Kulkzer Technovit manufacturers website provides you more information or you could contact their specialists directly through this link: http://kulzer-technik.de/en_kt/kt/maerkte/histologie/startseite_histologie.aspx Yours, Ronald Mit freundlichen Grüßen/Kind Regards Ro