Re: [Histonet] Detached vinyl coverslips - any advice on reattachment?

I should have mentioned that the section invariably remains attached to these vinyl coverslips when they become detached from the slides. If they had remained on the slides we wouldn't have a problem as we are used to restoring much older, Victorian slides that use Canada Balsam as a mountant.

[Histonet] Histology Supervisor Position

Clinical Laboratory of the Black Hills is an independent pathology practice providing anatomic and cytologic services to Rapid City, South Dakota and surrounding communities. Rapid City is the gateway to the Black Hills and offers a variety of four season, family friendly activities. Our

[Histonet] Detached vinyl coverslips - any advice on reattachment?

I'm an amateur microscopist and along with a couple of fellow club members we have acquired a fairly extensive collection of histopathology slides (approx. 2500 slides) made in the early 2000s and used for seminar and lecture purposes. We intend to use these to further our knowledge and interest

[Histonet] EBV control slides

Michele, Hope this helps! http://www.biosb.com/biosb-products/infectious-disease-arrays/ BSB 0237Epstein Barr Virus Cell Line Array (2 Core) 5 slides Regan Fulton, M.D., Ph.D. CEO, Array Science, LLC 475 Gate 5

[Histonet] IHC control slides for EBNA

Does anyone know of a source of IHC control slides for EBV Nuclear Antigen? Thank you. Michele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Inconsistent H & E Staining

A quick question: Are you using any recycled reagents? ie xylene, alcohol John www.ciqc.ca ‐‐‐ Original Message ‐‐‐ On Wednesday, November 14, 2018 12:24 PM, MONICA D. LOCKHART via Histonet wrote: > Hello Histo Fam!! > > Please help! > > My facility is experiencing frequent H & E

Re: [Histonet] Histonet Digest, Vol 180, Issue 7

Hello, An update on my question: On Fri, Nov 9, 2018 at 12:22 PM wrote: > > Message: 1 > Date: Thu, 8 Nov 2018 15:45:35 -0600 > From: Tyrone Genade > To: histonet > Subject: [Histonet] counter stains for Sudan Black B > Message-ID: > < >

[Histonet] Cryosectioning Brain problem

Hello, I am wondering if anyone has any tips on how to get smooth sections of fixed frozen sections of mouse brain. We are getting small wrinkles throughout. Thanks for any input. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] p2y12 cryo rat problem high background

It could be the concentration of the antibody is too high. Have you tried a lower dilution? What species made the antibody? I know that sounds a bit basic but I know that I have used a mouse or rat antibody by mistake. Paula Sent from my iPhone > On Nov 15, 2018, at 4:44 AM, Kooijman,

Re: [Histonet] p2y12 cryo rat problem high background

Hi Esther, I worked extensively with brain and spinal cord sections in the past, in multiple species. You need endogenous quenching step. I would always make my own (commerically available components yielded different results). I used a 0.09% H2O2 in 6% Triton-X 100: (formula: 200mL 1x PBS + 6mL

Re: [Histonet] p2y12 cryo rat problem high background

Hello Bobbie, But should I elimination endogenous peroxidase activity in brain/spinal cord tissues? Brains and spinal cord was harvested after cervical dislocation, then the tissue was snap frozen (isopentane..). thanks for your help, Esther -Oorspronkelijk bericht- Van: Boyce, Bobbie

[Histonet] p2y12 cryo rat problem high background

Hello all, I am trying to stain cryo brain sections from the rat 7um but having a lot of background. What am I doing wrong. Below the protocol is used and tried to adjust… 1- Fix the tissue with cold acetone (-20 ⁰C) for 10 minutes 2- Let the slide dry for 30 minutes at room