Re: [Histonet] Antibody diluent

Hi Scott, It may be best to call CAP to get an interpretation. However, I think that since you would be changing diluent for predictive markers, a full validation may be required. Best, Dan Daniel Pesino, HTL(ASCP)QIHCCM Senior Histotechnologist Trinity Health Of New England daniel.pes...@tri

Re: [Histonet] Alcian Blue staining

Hi Charles - your control tissue may be autolyzed. Try using a well-processed control tissue. If that's not it, make sure that your control tissue is being completely deparaffinized and that it is being totally covered with the stains. Good luck! Daniel Pesino, HTL(ASCP)QIHC CM Senior Histotech

Re: [Histonet] [External] Tissue processor advice

Hi Colleen - I've used both and I enjoyed both machines. The Leica instrument feels a bit more modern and the reagent management system is useful for reagent changing schedules. The changing of reagents and wax is pretty easy with the drainage hose. I've rarely had any issues with them, but hav

Re: [Histonet] [External] QIHC Prep

Hi Ariel - Congratulations on this big step in your career! I used the QIHC Prep Course program by Joe Myers. The course is excellent and comes with a very helpful resource guide. You can find it on the NSH website. Best of luck! Daniel Pesino, HTL(ASCP)CM QIHC CM Senior Histotechnologist Trin

Re: [Histonet] [External] Leica Bond, ASP6025 Processor parts

You should see how much they want for a wax drain hose for the Peloris processors... A tube and a nozzle for +$1,000. I ended up building my own with parts from Home Depot for about $30. We use Perkins Biomedical for our preventative maintenance and parts. They do repairs throughout the northea

Re: [Histonet] [External] IHC DAB

Hi Kristy, What platform and detection kit are you using? Would you mind sharing your DAB protocol? Thank you, Daniel Pesino, HTL(ASCP)CM QIHC CM Senior Histotechnologist Trinity Health Of New England daniel.pes...@trinityhealthofne.org W  860-714-4675 114 Woodland Street Hartford, CT 06105 T