Re: [Histonet] Causes of false positive Congo Red

.. 50ml Sodium hydroxide 1%... 0.5ml Filter and use within 15 minutes. On Thu, Jun 20, 2024 at 9:31 AM Greg Dobbin wrote: > Good day John, > Very nice to hear from you again! I have been consulting your textbook in > my investigations! > Sorry about the brevity of the

Re: [Histonet] Causes of false positive Congo Red

re they using "at another > lab" to get correct red amyloid that is green (dichroic, not fluorescent) > with crossed polars? > *John Kiernan* > > https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html > = = = >

[Histonet] Causes of false positive Congo Red

te ethanol, 6 dips each. Dip the slide 10 times in a coplin of xylene. Continue dehydrating the other slides. Coverslip the slides. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 ___ Histonet mailing list Histonet@lists.utsouthw

Re: [Histonet] IHC Validation Question

pieces of tonsil, pancreas, small bowel and liver. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tisse disposal/formalin

Decant formalin off into formalin waste containers and the remaining tissue go into a red bag (red signifying destined for incineration). As the O.R. does with fresh tissues for disposal. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0

Re: [Histonet] ISH Decal specimens

Use a formic acid based decal solution and MAKE SURE the specimen does not fix (formalin fixation) longer than 32hrs (false negatives result). Use 70% ETOH to manage the fixation time (ie fix for 24 hours, decal for 4 hrs, place in 70% ETOH until it gets put on the processor. Hold in 70% ETOH for

Re: [Histonet] destain for IHC

and then run slides on your IHC platform as you would -without the dewax step obviously. :-) *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *If God made a drink better than whiskey he kept it for himself! * ___ Histonet mailing list Histonet

Re: [Histonet] cd138

We use the same. Our protocol is ER2 for 15 mins. We have 3 washes after polymer. Greg *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *If God made a drink better than whiskey he kept it for himself! * ___ Histonet mailing list

Re: [Histonet] Processing artefact??

Since images cannot be attached, if any of you feel you have some experience that I may find helpful...please email me directly and I will attach the image to my reply. Thanks again, *Greg Dobbin* Chief Technologist Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE, Canada

[Histonet] Processing artefact??

! Any ideas? Thanks, *Greg Dobbin* Chief Technologist Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tissue staining artefacts - areas of tissue not labelling

the control section perform? As expected or same artefact? *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *If God made a drink better than whiskey he kept it for himself! * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] ultrasafe formalin dispense

issue discards. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] formalin in OR

specimens are split between Cytology and Histology...the histo specimens go directly into formalin. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list

[Histonet] Fixation time and ISH

until they can be processed into paraffin. My questions: Is anyone else doing this?? Is 70% ETOH still a viable option for labs in this situation?? And… Is there another idea and/or more information out there that could help us in this regard?? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove

[Histonet] Cold ischemic times

director was looking for suggestions for quality indicators to report and while I feel like our cold ischemic average time is impressive at ~17 mins, she says that is pretty standard for everyone.* Thanks, Greg -- *Greg Dobbin* Chief Technologist - Anatomic Pathology Queen Elizabeth Hospital

[Histonet] New cryostat validation procedure

, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Water under sections

unostainer. Hope this helps. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.e

Re: [Histonet] IHC validation after a new tissue processer is installed

comparisons between old and new processors (the downside of what is otherwise an exciting time!). Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list

Re: [Histonet] Blank Spots on IHC- Bubbles on Bond

e reagents then it is a defective valve or line for that reagent. I hope this was helpful. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing lis

Re: [Histonet] process formalin-fixed tissues from animals infected with a virus

Garratt wrote: > Evaluation of Virus Inactivation by Formaldehyde to Enhance Biosafety of > Diagnostic Electron Microscopy > > > https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353909/ > > > It is nice to have a reference. > > > > John > > On Thu, Aug 6,

Re: [Histonet] process formalin-fixed tissues from animals infected with a virus

Hi Amy, Formalin fixed tissue is no longer infectious...unless you are talking about prions (eg scrapie, BSE, etc). So there should otherwise be no concerns or additional precautions required. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything

Re: [Histonet] Spectra Staining Kits

, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tissue Tek embedding centers

that switch should be an easy and cost-effecient thing to try first. Good luck, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet

Re: [Histonet] IHC troubleshooting

stain) would likely remove parts or all of the section. All the best, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet

Re: [Histonet] IHC Troubleshooting

not drain out on standing and does not drain or evaporate when baking at 60C. If the slide with trapped water is put on the instrument with trapped water, the dewax step will lift much of the tissue. Cheers, -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything

Re: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline

ons with the same AR (eg 1:10, 1:25 and 1:50). Once you get close to where you think the intensity should be you can try modifying the AR protocol to improve the sensitivity. Good luck, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation its

Re: [Histonet] Processing Schedule- ASP-6025

tonsil kept in > formalin before processing? Could over fixation be the issue? > I have found that maintaining tight control of control tissues is > important which includes minimum and maximum fixation time. > > John > > Sent from ProtonMail Mobile > > > On Tue, May 7,

[Histonet] Processing Schedule- ASP-6025

this schedule that is particularly concerning?* Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] Histonet Digest, Vol 184, Issue 23

think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___

Re: [Histonet] ER/PR question

l for both ER and PR. It seems to me that if the internal control is staining adequately, then perhaps interpretation will not be an issue. I am curious, does anyone have a different take on relying the internal control as a guide in this particular situation?? Greg -- *Greg Dobbin* 1205 Pleasant G

Re: [Histonet] IHC-H HELP...

and no additional retrieval. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] Question - EM

Hi Dr. Cartun, It has been many years since I worked in EM but I my recollection is that tissues could remain in 2% Glut indefinitely without detriment (for EM purposes). However, Osmium tetroxide had to have a limited exposure. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A

Re: [Histonet] Leica Bond Max artifact

? Could a new person be putting undiluted Bond Wash in the bulk container? Let us know what you find out. Good luck. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself

[Histonet] Braf protocol on Bond-III

1:100 with ER-2 for 30 mins. Clone is the V600E from Spring BioScience Looks great for uis and we are performing well on EQA surveys. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself

Re: [Histonet] BRAF

Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 > (high pH) for 30 mins. > > Greg > > > > -- > > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > > *Everything in moderation...even moderation itsel

Re: [Histonet] BRAF

Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 (high pH) for 30 mins. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself

Re: [Histonet] USA to Canada

Pass The General MLT Certification exam, as our MLT exam includes Histology and Microbiology. :-) Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list

[Histonet] Slide and block retention -Peds seperated?

cases. Thank you for your time! Greg -- *Greg Dobbin​, MLT* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 ___ Histonet

Re: [Histonet] Leica open containers

of intensity over two years even. For me, the antibody expires long before the any deterioration of signal is noticed. In your case, if you are using a commercially prepared diluent, then start looking at extended times left out of the fridge, a malfunctioning fridge, contamination, etc. Greg -- *Greg

Re: [Histonet] p16 Antibody

Hi Laurie, We are purchasing the antibody only from Roche/Ventana and optimizing it for use with our detection system (Bond Refine Detection kit) and the Bond-III stainer. Ask your Ventana person about buying the Ab only. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0

Re: [Histonet] Paraffin infiltration issue, will this impact IHC?

not be an issue if you are able to obtain good sections. Have you stained these poorly infiltrated specimens with H? Are you certain you have good sections with all tissue components represented? That would be my main concern. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0

Re: [Histonet] Leica embedding station

the drawer. Hope this helps, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailma

Re: [Histonet] Antibody Validation CLIA

with your Director to find that balance between cost (ie more slides = increased cost) with confidence (how many do you need to see in order to feel confident that the stain is performing as expected). I hope this helps. Sincerely, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE

[Histonet] re; cell blocks and frozen for IHC

No pretreatments for anything that is not formalin fixed. I think 95% ETOH for a fixative but but others may have a better idea than I. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself

[Histonet] AFB Stain on Cytospin Preps

have any adverse affect on the stainability of AFB organisms if present? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet

Re: [Histonet] Kappa and Lambda by ISH

marrow specimens only stains plasma cells it does *NOT* stain B-cells. So this is likely why LN tend to be negative. Maybe one of our Histonet-contributing pathologists would like to elaborate? :-) Greg -- *Greg Dobbin* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen

Re: [Histonet] Kappa & Lambda ISH

ould be for me to share our protocol. Let me know if you would like me to send that to you. Cheers, Greg -- *Greg Dobbin, R.T.* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902)

[Histonet] Storage of blanks (Charged slides)

, in a box, wrapped, stacked, etc)? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Section adherence issues (IHC)

before processing. We use the same slides for Special Stains and don't have this issue there. I need some new suggestions to try! All ideas welcome. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself

Re: [Histonet] Releasing of Patient Tissue

for pick up. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] grad student problem

in the freezer for a little longer to avoid freezer burn. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Uneven ER/PR

tact Leica Tech support to try to figure out what went wrong the first time. If it is not corrected, then you are back to suspecting a pre-analytic issue (assuming you are not seeing this uneven-ness in other IHC specimens). I strongly encourage use of tissue controls on every patient slide. Cheers, Greg -

[Histonet] IHC Staining (Pardue, Judith)

Judith, The red chromagen is suseptible to "fading" if stained slides are left in either alcohol or xylene for too long. Check to make sure that everyone involved is following the same procedure for the dehydraytion, clearing and mounting of the stained slides. Greg -- *Greg Do

Re: [Histonet] Safety question

Buy yourself a roll of WHMIS labels. Get ones that are sufficiently large to be easily read while still fitting on the side of the secondary container. All necessary info will be captured there once you check the right boxes, etc. Cheers, Greg -- Greg Dobbin, R.T. Chief Technologist, Anatomic

Re: [Histonet] Microtomy Statistics

) benchmark for all to strive for as their minimum. Cheers, Greg -- Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even

[Histonet] Looking for fine mesh cassette

that will work with my printer?? Thank you, Greg Dobbin Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself

Re: [Histonet] Light hematoxylin staining in HE

Are you soaking your blocks in dilute ammonium hydroxide before sectioning? Soaking too long will affect stain quality. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894

Re: [Histonet] negative controls on immunos

really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work

RE: [Histonet] negative controls on immunos

Thanks Joyce. This excerpt supports what I had said- noting the difference between the negative (or deletion) control (which is what I was addressing in my reply) and the negative tissue control. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine

RE: [Histonet] IHC Equipment

The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen

RE: [Histonet] Any problems with Bond Max Heater Assembly (SSA)

the other 9 positions remain functional. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem

Re: [Histonet] ihc slides

Cynthia, Hematoxylin will inactivate the peroxidase (link) in the tissues. You must go back and re-do the link step and hopefully the antigenic sites will still accept more streptavidin (or similar) conjugated antibody. Cheer. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept

Re: [Histonet] ihc slides

I should add that the DAB step may also have quenched all of the linked peroxidases even before the hematoxilin was added (which would finish off any that remained). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box

[Histonet] glycogen degeneration???

some feedback on this one. Thanks so much, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I

Re: [Histonet] Klotz fixative

as far as I am aware. I have no experience with the affects on histological preparations. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find

Re: [Histonet] Advice from Canadian Labs

Hey Pam, No problem, I will. Nice to hear someone south of our border has heard of Canada's smallest province!! :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894

[Histonet] Advice from Canadian Labs

reports, 2 years? 20 years?? What are others doing in this regard? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find

Re: [Histonet] Cutting Frozen Lung

experience. good luck. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas

Re: [Histonet] opinion please

I agree with Renee. It will proabaly be at least as costly to have to re-validate everything! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385

[Histonet] Morgue topic: Chain of Custody for personal effects

. Thank you! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson

Re: [Histonet] Leica Bond/Novocastra Reagents

Almost always receive on time. I can think of only one instance in a year and a half where anything was on back order (it was an antibody). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A

Re: [Histonet] mib-1

Alcohol based suggests to me an absence of formalin. In which case, epitope retreival is not indicated. Also, you didn't say if it had been working and stopped or if this was a new marker for your lab. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine

RE: [Histonet] Best IHC stainer

I echo Joyce's comments! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have

Re: [Histonet] STD testing in Lab

drab Wednesday afternoon. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have

RE: [Histonet] STD testing in Lab

Oh my gosh! You're right. My day is suddenly not so drab!! LOL Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work

RE: [Histonet] Leica CV5030

-coverslip slides. But since I switched to using the premium grade year round (as opposed to only in the more humid months, which is what I was doing to try andsave money!) I have had almost no glitches at all! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept

[Histonet] Cracked or shattered LN sections

trying to make sure that the tissue is flat in the mold (ie pressing the tissue down). Has anyone else out there have any similar experience with this problem? Is the solution as simple as cutting smaller pieces into the cassette at the time of trimming? Thanks. Greg Greg Dobbin, R.T. Chief

RE: [Histonet] Cracked or shattered LN sections

I am positive no freezing spray being used. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I

Re: [Histonet] Recycled formalin

Yes. ?? Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson

Re: [Histonet] Commercial Eosin

Hi Vanessa, Yes! I have our eosin staining down to just 10 secs! I am considering just letting the slides hover over top of the eosin for a minute instead of actually dipping! LOL Very strong indeed. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen

Re: [Histonet] IHC stainer

I echo everything Julie said! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have

RE: [Histonet] IHC stainer

I guess I would have to echo that as well! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more

[Histonet] Embedding bone marrow aspirates

for subsequent processing. I look forward to reading your knowledgeable replies. Have a nice weekend everyone. Sincerely, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894

Re: [Histonet] BVD Antibody

300. Format: Mouse ascites Size: 0.1, 0.5 and 1 mg Expiration: One year from date of QC release. Storage: Store at 2-7°C. Do not freeze! Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5

Re: [Histonet] Glass coverslippers

The CV5030 works very well for us-as long as we spend a little more buying the higher grade coverslips that don't tend to be affected by humidity as much. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600

Re: [Histonet] IF on cell lines

). Hopefully the 4% paraform. works. Is methanol fixation an option?? Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I

Re: [Histonet] IHC and ISH

instruments (they know who they are) require you to buy an entire separate detection system to do ISH and so if your volumes for ISH are low, you will waste a lot of money in expired kits. Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth

[Histonet] Cytology Autostainers

Hi Folks, What's out there? What's hot and what's not? Anyone using their cytology autostainer for histology special stains? Welcome all input (including vendors). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O

Re: [Histonet] Auto-IHC Staining Issues

Hi Paula, You really have to tell us which instrument you are using to get a concise answer. Other details like how the slides are dewaxed for instance would also definately help. Regards, Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth

Re: [Histonet] double staining on the ventana XT

Hi Jennifer, Your Ventana Application Specialist should be more than willing to help you thru this process. I demo'd an XT last year and I recall double-staining was very straight forward. Unfortunately the details of which were not retained by me! Good luck. Greg Greg Dobbin, R.T. Chief

RE: [Histonet] Presidential Voting Infomation

accomplished his mission! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 And in the end it's not the years in your life that count. It's the life

Re: [Histonet] Protocol: Saving unstained slides...

of this response necessarily addresses either CAP or CLIA regulations). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Success