Cell Marque has one that I have used in formalin fixed paraffin embedded
tissues. We use the Dako Flex detection system. Antibody diluted to 1/50.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
Curious as to why you wouldn't worry about prions ?
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: Rene J Buesa [rjbu...@yahoo.com]
Sent: Monday, October 08, 2012 12:03 PM
To: McMahon, Loralee A; Histo Histo; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet
No we are still doing the triple stain, just stopped billing three.
The pathologist decided that the patient specimen was more valuable.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
We use the Leica Biosystems X-tra slides with a lot of success for Brain and
bone.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
I don't pay $1 per slide for the leica slidesso you might want to ask your
salesrep.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
We buy rubber cement from walmart or staples and seal the outside of the
coverslip before the hybridization step. And we have had much success with
minimal probe.
But I understand, I cross my fingers when I do large resected pieces of liver.
AND I keep on complaining to the vendors that
I am hoping that the vendors will recognize this when the make these
agreements.
Hopefully not doubling our prices!
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
Roche/Ventana. they bought MTM labs and then raised the price.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
What was your tissue decaled in?
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
I think that the original question was why is it recommended in some procedures
that H202 be diluted in methanol. And others say distilled water or buffer.
What makes one better than the other?
Some people I have worked with insist on getting 30% h202 and diluting it in
methanol to a 3%
This is where I get my stuff for my TMA machine from
http://www.estigen.com/
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
They are still selling it. But they do not know for how long. And because it
is a legal issue they can't tell me whom is going to be the sole provider
either.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
Sorry Teri must have missed the reply all button.
And also Lablion claims to interface with any current lab equipment that you
have. So if you already have slide printers (either etchers or labelers) it
can work. Like I said. I haven't seen it in action, but I sure would like to.
Loralee
Calbiochem p21 (1:100) and Cell Signaling for the p27 (1:250)
We use the Dako Flex Reagents on the autostainer. Low pH retrieval.
I have more detailed protocol if you need it.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
If you are embedding skins and they all have to be on edge. And some of them
are bisected or tri-sected and you have to put two to three pieces of skin in
one cassette all on edge. I would say a good tech could do about 45-50 per
hour.
If they were all punches, then 60 would be about right.
Biocare has it. IVD...
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On
I am attempting to work up alpha2 beta 1 intergrin from abcam.
I have tried lots of things, But if anyone has any suggestions to get it to
work I would appreciate it.
Even if it means getting a different antibody.
thank you.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong
We used to make our own. We would get a large shallow cardboard box, fill it
with paraffin. Let it cool. Then peel the cardboard away
You can submerse that in formalin. And you can custom cut them to whatever
size you need.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
We use it and find it very reliable. We have also found that the control
tissue can lose its antigenicty if cut too far in advance and allowed to sit,
mostly for the ER. Haven't seen this drop off with the PR.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial
We are moving to timing everything. From ishcemic time (time out of patient to
time in formalin). And also the length of time in fixation. More so that we
can track something back if there is a problem anywhere along the line.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A
[loralee_mcma...@urmc.rochester.edu]
Sent: Tuesday, April 03, 2012 1:14 PM
To: Kay W. Nabers; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: GI biopsies
We are moving to timing everything. From ishcemic time (time out of patient to
time
This is exactly what we do as well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
I would think that if NYS is involved in anyway you have to hold those blocks
for 20 years. When they inspect you do they inspect both labs? Do both labs
hold a NYS permit?
For cetain I would call NYS before you discard anything.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
We use the same antibody, but use PK for 5 mins. not HEIR.Very clean
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
What kind of slides are you using? I have had bad lot numbers of slides. New
lot number cleared up the problem.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
We have 17 histotech over 1000 lab techs ( classified as Medical Technologists)
and NO union
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Looking for either IVD or ASR antibodies for
MDM 2 and CDK4.
Vendors ok to respond.
Thank you.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
___
Histonet mailing
I haven't heard that one. Do they mean present physically or present as in
their office reading slides, but a phone call or page away?
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
Are you prepping the breast tissue upon receipt? We bread loaf our larger
peices and place into formalin in large containers to fix overnight.
Rarely do we have a reprocess a block. Mostly we find that the reprocessing
comes from the peices of tissue that are being put into the cassettes are
Hi Histonet,
Wondering if anyone out there would be willing to share a company and protocol
for Galectin 3 and Trypsin IHC's on paraffin embedded tissue.
Thanks in advance.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
I have place the deparaffinized slides into 10% neutral buffered formalin for
about 30 minutes before the heat retrieval. Somehow this seems to work
You can also lower the temperature of your retrieval and lengthen the time.
Good Luck.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry
I have tried without much success. Leica (novacastra) has an antibody.
I have been told that it is not the antibody that is bad that it is the tissue
that doesn't always show positivity.
We tested many biopsies that were serum positive for HepC but the immuno was
negative.
If you get
Cell Marque
0.1 ml concentrated 367M-14
0.5 ml concentrated 367M-15
1 ml concentrated 367M-16
1 ml prediluted 367M-17
7 ml prediluted 367M-18
We use it routinely and it works great.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
We use Dako's Beta Amyloid with high pH heat retrieval. Works very well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
Hi Histonet.
Can anyone recommend a HHV-6 antibody to use in formalin fixed paraffin
embedded tissues.
Preferably ASR or IVD. But I'll take whatever suggestions.
Thanks in advance.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical
run them down to water or buffer and then leave them in buffer in the
refrigerator. They should be fine.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Rene,
I understand the desire to remove Xylene from the lab completely. But what if
you are running FDA approved kits that are only FDA approved if the tissue is
processed in Xylene?
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical
)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: Rene J Buesa [rjbu...@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE
Department of Surgical Pathology
(585) 275-7210
From: Rene J Buesa [rjbu...@yahoo.com]
Sent: Tuesday, September 27, 2011 11:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity
Loralee:
Poor people
environment.
René J.
--- On Tue, 9/27/11, McMahon, Loralee A loralee_mcma...@urmc.rochester.edu
wrote:
From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu
Subject: RE: [Histonet] Xylene sensitivity
To: Rene J Buesa rjbu...@yahoo.com, Histonet Listserv (E-mail)
histonet
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon,
Loralee A
Sent: Tuesday, September 27, 2011 2:59 PM
To: Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: RE: [Histonet] Xylene sensitivity
We did
Thanks Steve,
Now I don't feel as if I am trying to slowly kill my technician!!
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
That is what we do as well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu]
What platform are you using and from what company is your myoD1
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
http://www.labsafety.com/lysol-ic-quaternary-cleaner-disinfectant_24530427/
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
We have been using an aqueous mounting media from Diagnostic Biosystems.
http://dbiosys.com/
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
I believe that they also need to be supervised at all times. They cannot be
left unsupervised, like on weekends or evenings.
Please check this out, I know that our gross room recently had to change things
due to the change in the resident rules.
Loralee McMahon, HTL (ASCP)
Hi We are in the process of getting a new cyrostat and would like some opinions.
We are looking at the ThermoFisher HM550.
Please feel free to contact me offline if you like.
Thank you in advance.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
Hi Histonet,
Can someone from the University of Pittsburg immunohistochemistry department
please send me an email. One of the pathologists here wanted me to find out
what you all do for Hep C. Do you perform the immuno or other?
Thank you in advance.
Loralee McMahon, HTL (ASCP)
We heat ours to 99C for 20 minutes with a cool down. Using the Dako PT link.
(water bath)
BUTbefore you change your pretreatment
Make sure that your tissue is fixed properly, processed properly.
After it is cut, make sure that the slides dry before you baked them in the
oven.
Any water
you have to remove yourself. follow the instructions.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
MTM labs is the only place to get it IVD.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
That is what we are using here. We are no longer using the HPV antibody. It
never really worked well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
I avoid anything that contains carmine be it yogurt or make up. !! I really
don't want bugs eat bugs or put them on my face.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
Biocare Medical
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
We currently do. And we have the Dako system. So everytime we print the IHC
reports it takes pages and pages. The pathologist are supposed to sign off on
them and return them to me. I am then supposed to file them. But...
The major problems are
it takes up hundreds of sheets of paper
That is a huge question. Here is a good place to start
NYS Clinical Laboratory TechnologySep 9, 2009 ... Clinical Laboratory
Technician. License Requirements ...
www.op.nysed.gov
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical
you could do a melanin bleach
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu]
We have actually performed a bleach step before staining. The only affect I
have seen ( and we have only done a few antibodies) is that the tissue gets
pretty chewed up. Especially if there is a pretreatment step prior to the IHC
staining.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry
We use the PT link, which according to dako is considered a waterbath.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
Take your total number of Breast cases and divide by the postitive ER cases to
get a percentage of cases.
Do the same for the PR and the HER2.
100 breast cases divided by 20 positive ER cases gives you a five percent
positive.
Your percent positive cases should closely correlate with the
...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A
[loralee_mcma...@urmc.rochester.edu]
Sent: Monday, March 07, 2011 3:20 PM
To: wanda.sm...@hcahealthcare.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: A Spreadsheet for ER/PR Benchmark Comparison
We usually run them on our automated stainers but I have been known to do them
by hand in a pinch
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
From: Jay Lundgren [jaylundg...@gmail.com]
Sent: Tuesday, March 01, 2011 3:10 PM
To: McMahon, Loralee A
Cc: Heather Cooper; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HELP! H. Pylori Immunos
Our problem, in my opinion is a control issue and not a staining one..our
Dr. Cartun,
We are using the C3d and C4d from Cell Marque. On the Dako platform.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
You have to get permission to dump it from you local water people.it varies
by location/state laws etc.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Biocare, They have a prediluted. Works great.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
It's been a while but I think that we used to add a little diluted glacial
acetic acid to our cytology specimens to lyse the red cells.
Not sure on the dilution, but maybe someone else knows.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
We use an aqueous mounting media from Diagnostic Biosystems. Available in two
sizes. Same as the aqua mount
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
We do this by hand. Usually it involves a summer student to compile all of the
data on a spread sheet and a formula to compute the percentage of positive
cases.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
I have been using the RISH from Biocare and you can use it on Decaled
specimens. they have a slightly different protocol (pretreatment) but we have
found that you don't need to adjust with our form of decal.
Not completely finished with the validation process on these, but that is what
we have
Hi Histonet,
I have been trying to work up HCV antibody without much success. Anyone out
there successfully doing this that would like to share their protocol with me?
I have many cases that have been diagnosed with HCV infection, so the control
tissue is not an issue.
We are using the Dako
If our cases are needle cores they are almost immediately put into formalin
from the patient. That time is recorded by the nurse or the clinician that
took the specimen.
The larger breast samples are received fresh from the OR and are immediately
grossed either by a PA or resident. Those
If you want the IVD form of the antibody you have to go with MTM labs, they
hold the patent. We buy the kit and take out the antibody and use the
detection for research. It works very very well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to
work on formic acid decaled bone. I also believe that there is an antibody for
osteoclasts, but I have not tried it.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department
is pretty good for Osteoclasts but
I do westerns in a research setting..has anyone had good success
with any antibody for immunostaining?
Best,
Praveen Arany,
Graduate Student,
Harvard University,
Cambridge MA
On 10/6/2010 8:43 AM, McMahon, Loralee A wrote:
You can do a TRAP stain on EDTA decaled
Milestone Medical has a really neat system. www.milesonemed.com It is called
the TissueSAFE. You will find it under preanalytical tools. We would love to
get one for our couriers and biospecimen repository. We haven't purchased it
yet.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry
We use a thermometer that will indicate to us if the temperature went high over
the weekend. There is a little group of pellets at one end that will drop to
the other end if the temperature is out of range. We call them Visual
Indicator. You can either buy them through one of the major
We are using a Lung Adenocarcinoma as a daily control. Although I have noticed
that normal lung macrophages will pick it up.
We ran our validation of the antibody using a multi tumor block (contains
various tumors from various organs) and a multi normal tissue block to ensure
that it stained
at
histonet-ow...@lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...
Today's Topics:
1. RE: CD34 positive control (McMahon, Loralee A)
2. Ventana Renaissance Containers (ricky hachy)
3. CD34 Control (Silverman
Just about any tissue should work. It stains for Hematopoietic
stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic
fibroblasts
We use a sausage style control here with about 30 different types of tissue.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
We use the same antibody from Cell Marque on the Dako Autostainers with great
success.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
We use Dragon here as well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu]
How big are these rat brains? Are you processing by hand or with a machine?
We routinely process pieces of human brain for about 10 to 11 hours on a VIP.
This seems like a really really long cycle. No heat and no vacuum until the
paraffin infiltration step. (formalin, 70% EtoH, 95% EtoH,
Hi Dr. Cartun,
We are running the HPV Genepoint test from Dako (in situ test). Along with the
EGFR IHC.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Use the RTU from Biocare. It is clean and easy. You can use it with the Dako
Flex Kit.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
I once took tissue that had dried up after being fixed in formalin and soaked
it in normal saline to rehydrate it. Very small piece of skin that I soaked
for about 3 hours.
Then placed the tissue back into formalin and reprocessed routinely.
It did not have the best morphology but is was
I think that CAP means that you need to save the slide that you ran from the
previous lot and compare it to the slide that you have stained with the new lot
number. To see if they are sufficient diagnostic quality. Not put both lot
numbers on the machine at the same time and then compare the
We have been using an INI-1 from Cell Marque. With a 1/50 dilution and a low
pH heat retrieval. Although I accidently had a slide go through the higher pH
heat retrieval and I didn't see a big difference in the staining.
I use the Dako system so it was a 30 minute incubation with a linker
We use Surfactant from Biocare at 1/100 dilution 30 minutes incubation, but we
use a lower pH retrieval 6.0 for 20 minutes at 99C.
I haven't had any trouble with the Cyclin D from Dako, we use the Ready to Use
version though.
Hope that helps.
Loralee McMahon, HTL (ASCP)
Hi Histonet,
Wondering if anyone has ever used a antibody for Hep C on formalin fixed
paraffin embedded tissue?
If so where did you get it.
Thanks in advance.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
Any inspection that I have undergone we have used the 25 to 30 case rule.
Except for the Er/Pr//Her-2. We use closer to 50 cases. We also use a TMA to
make our lives easier. The TMA contains known positives and known negatives.
In cases of t-cell or b-cell markers or cytokeratins. 25 to 30
From: Mike Pence [mpe...@grhs.net]
Sent: Wednesday, April 28, 2010 3:10 PM
To: McMahon, Loralee A; thisis...@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Responses to IHC CAP Validation question
I know you keep the paper work showing what you have done, but do
I am not sure what the problem is here. You have to fix for up to 72 hours no
more you can do less time. If you are already fixing your her2 for 48 hours,
then what needs to change? You are not going to submit different cassettes
for Er/Pr to fix for longer? You will submit them all and
Department of Surgical Pathology
(585) 275-7210
From: Mike Pence [mpe...@grhs.net]
Sent: Friday, April 23, 2010 10:58 AM
To: McMahon, Loralee A; Patsy Ruegg; Morken, Tim; Maxim Peshkov
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new er pr cap
They have commercial rapid decals that are formic acid based that do not affect
the immunohistochemistry. But it does affect some of the enzyme
histochemistry.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585)
They will send you a copy of the checklist that you will be inspected under
before your inspection. They do not publish it that I know of.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
I have upgraded to the new autostainer links. I have four of them. There are
many pros and many cons. If you have any specific questions I'd be happy to
answer.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585)
I have upgraded to the new autostainer links. I have four of them. There are
many pros and many cons.
Too many to list. If you have any specific questions I'd be happy to answer
them.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of
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