hahaha that's pretty clever, actually!
On 6/14/2011 6:56 AM, Paula Sicurello wrote:
This reminds me of a joke I learned as a kid. How to use analyze and
anatomy in a sentence (song actually).
My analyze over the ocean,
my analyze over the sea,
my analyze over the ocean,
so bring back my
I'm guessing, then, that anatomal and pathalogal aren't words...?
On 6/13/2011 2:51 PM, Jan Shivers wrote:
When taking coursework eons ago, one of my professors addressed this
very issue. He said that '-ic' and '-al' were BOTH adjective
suffixes... and there was no need to include both at
**Listserv master: Some people are reporting problems with managing their
subscription as they have submitted their unsubscription multiple times in
the prescribed manner and are still getting Histonet emails. This was
reported to me by one such (failed) unsubscriber.**
--On Thursday, April
I concur with the others. For about 6 years I've made it from powder but
the toxicity risk bothered me until I realized how safely and cheaply you
can just buy the same stuff. We get a discount through our stockroom on a
4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28.
I
We had the EXACT same problem about a year ago! We don't believe we had
storage issues, either. We've been using SlowFade Gold (Molecular Probes,
Invitrogen) for about a year now and it's been a great aqueous mounting
medium. We add our own DAPI (2ul of a 1mg/ml DAPI solution per 500ul
AccuEdge by Sakura Finetek are the best (avail. through VWR)
Not only have I tried a few other brands, but our former knowledgeable and
experienced histotech here at UB recommends AccuEdge.
Merced
--On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick
barricksta...@yahoo.com wrote:
I am looking for a good antibody for MAC-1 (CD11b/CD18, Integrin alpha-M
beta-2) that is targeted to rodent tissue.
Any ideas where I can find one?
Thanks!
Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of
I always find it interesting to discover the vast sea of temperaments out
there that is elucidated so clearly in the light of email responses. The
same email can provoke anger, inflict hurt, or elicit laughter from
different people. :-)
With that in mind studies have shown (so I'm told by a
We don't let our unfixed sections air-dry (room temp) long before fixing
them - a few minutes? For some people here I let them sit a few minutes
then just store them in the -80 for up to a weeks weeks before they fix
them and commence with their staining procedure. If the animals were
sorry, that should read for up to a few weeks.
--On Tuesday, March 24, 2009 1:00 PM -0400 Merced Leiker
lei...@buffalo.edu wrote:
We don't let our unfixed sections air-dry (room temp) long before fixing
them - a few minutes? For some people here I let them sit a few minutes
then just store
1. Generally 24 hrs for 2-3 mm thick piece. At least a 10:1 formalin:tissue
volume.
2. Room temp or 4oC - I've heard debates, and I've heard it doesn't matter,
but we do it at 4oC.
--On Tuesday, March 24, 2009 4:54 PM -0400 Jacqui Detmar
det...@lunenfeld.ca wrote:
Hi all. Having a bit
Now I'm interested in how you embed so as to not have to scrape...if i
don't add enough wax (enough to end up rising around the edges of the
cassette, hence the scraping later), i don't get a secure hold of the block
to the cassette...
ML
--On Thursday, March 19, 2009 7:37 AM -0700 Martin,
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: Merced Leiker [mailto:lei...@buffalo.edu]
Sent: Thursday, March 19, 2009 10:56 AM
To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce;
Histonet Subject: RE: [Histonet] Paraffin Block trimming
Now I'm
and paraffin over the years...same
result.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: Merced Leiker [mailto:lei...@buffalo.edu]
Sent: Thursday, March 19, 2009 11:16 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED
lol...I wanted to say the same thing...and how in the world can you even
see it when you section it? How do you stain it without losing it from the
slide? Is there really enough surface area for it to adhere and withstand
washing? ...and it's ALMOST Friday...!
--On Thursday, March 19, 2009
Definitely do at least that one sucrose cryopreservation step you
mentioned. Even step up to it in 10% and 20% sucrose.
--On Wednesday, March 18, 2009 4:04 PM +1100 Adam Galle
adam.ga...@student.unsw.edu.au wrote:
Hi all,
Currently I am working with brains from 7 day old rat pups, that
I agree with Frances and anh2006. Cryospreservation is important for brains
especially and must be done after fixation, before freezing, and the
glutaraldeyde can irreversibly mask antigens.
Merced
--On Wednesday, March 18, 2009 8:49 AM -0500 Swain, Frances L
swainfranc...@uams.edu wrote:
I make 8um-thick sections of various tissues and sometimes they do curl. I
do not use an anti-roll plate. So what I do while making the section is to
cut just enough of the section so that it is attached by a very small
amount to the top of the block, (so that most of the block has gone down
Ugh. We are not allowed to dispose any chemical, including formaldehyde,
down our drains here at UB (Buffalo, NY). Everything goes into
appropriately-labeled waste bottles for Hazardous Waste to dispose of,
however they do it.
--On Saturday, March 14, 2009 12:05 AM +0800 TF ti...@foxmail.com
LOL! It's ALMOST Friday, people, ALMOST... :-)
--On Thursday, March 12, 2009 11:46 AM -0500 Jackie M O'Connor
Jackie.O'con...@abbott.com wrote:
Not Dako - Leica purchased Surgipath.
Burton, Lynn lynn.bur...@illinois.gov
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/12/2009
I have a refurbished Reichert-Jung 2030, but with a knife holder from a
newer model (so I'm told by the refurbisher). I use 0-1 degree angle.
Merced
--On Tuesday, March 10, 2009 4:15 PM -0700 Va Paula Sicurello
vapat...@yahoo.com wrote:
Hello Listers,
I have inherited a Reichert-Jung
We have a broken glass waste container we put ours in for pick up and
disposal (don't deal with non-animal patients, so not an issue).
-M
--On Wednesday, March 11, 2009 4:56 AM -0400 Sharon Campbell
shar...@celligent.net wrote:
Hi everyone!
Thank you for all the great responses to my last
What animal tissues are you using? If rodent, may need less time on the
steps - like 10-20 min.
--On Wednesday, March 11, 2009 11:29 AM -0700 Va Paula Sicurello
vapat...@yahoo.com wrote:
Hi Histo netters,
I am am my wit's end. I know how to section but my sections are
compressing like
I like my metal molds. They are neat, you get even heat transfer, and are
easy to clean when they need it. I clean them by tossing them in some
boiling water with an excess of cleaning powder (Alconox or Sparkleen) or
any detergent you have on hand (make sure it doesn't get too bubbly and
, the paraffin will harden and you discard
it without pouring down the drain...
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced
Leiker
Sent: Tuesday, March 10, 2009 12:22 PM
To: Sharon Campbell; histonet
Hi Vanessa,
I have read in at least one online protocol and have also been advised by
our excellent and experienced (former) histotech who used to be here at UB
NOT to warm the slides without air-drying first. We typically air-dry the
slides, yes, standing up, overnight, before warming them.
I've read that 4-5um is average, and that's what I typically go by for
paraffins, but then it could depend on tissue type as Rene recommended. I
end up having to cut frozens at 8-10um because for some reason it's very
difficult to cut any thinner on the cyrostat I use (an old Vibratome
ummm...distilled water works best, unless you like cleaning out mineral
deposit build-up and such...
--On Monday, March 02, 2009 11:18 AM -0800 kristen arvidson
arvidsonkris...@yahoo.com wrote:
Are most people using distilled water in the water baths?
In our experience, the heat is definitely more important...we retrieve
epitopes ranging from cytoskeletal markers (Troponins, Myosin Heavy
Chain,...) to nucler (PCNA) and others (c-kit, beta-catenin, vonWillebrand
Factor-vWF). We use a steamer set to 30', just place the slides in flat on
the
Thank you everyone who replied with a suggestion!!!
--On Tuesday, February 17, 2009 3:17 PM -0500 Merced Leiker
lei...@buffalo.edu wrote:
Hello Histonetters,
I am looking for a good CD45 (CLA) antibody for use in paraffin
immunofluorescence that meets the following criteria:
1
(I'm going to try asking this again, as I only got 1 response and it wasn't
what I was looking for. Thank you!)
Hello Histonetters,
I am looking for a good CD45 (LCA) antibody for use in paraffin
immunofluorescence (or ANY application at this point) that is of a
NON-mouse isotype (that is,
I have used them almost daily for a year or more now and have had no
problems with the slides giving off any autofluorescence in
immunofluorescence applications.
--On Thursday, January 22, 2009 2:56 PM -0500 Nancy Lemke
ns...@neuro.hfh.edu wrote:
Has anyone using Starfrost slides (7255)
LOL, Jackie!!!
I've used 10-year old strips that seemed to have worked fine.
--On Wednesday, December 31, 2008 12:57 PM -0600 Jackie M O'Connor
Jackie.O'con...@abbott.com wrote:
I heard of some that went on a graffiti rampage in downtown Chicago last
year . . . . .
Happy New Year.
Hey all,
While embedding rodent tissues I have found that some specimen within the
SAME muscle group (such as quadriceps) appear very dark in color while
others are very pale. I just recently started noticing this while I've been
embedding. (I embed manually; I'm a research tech who does a
Just to clarify, this is the same rodent muscle. We take out the whole
quadriceps from one rodent, the whole quadriceps from another rodent, and
both will look different colors while embedding.
--On Friday, December 19, 2008 3:03 PM -0500 Merced Leiker
lei...@buffalo.edu wrote:
Hey all
This indeed sounds like the most honest and integrous approach as well as a
way to keep positions competitive. I like it.
--On Monday, December 15, 2008 11:00 AM -0500 Terri Braud
tbr...@holyredeemer.com wrote:
I agree that if you have a great work environment with competitive pay
your
Oh, I'd like to know that, too, please!
--On Friday, December 12, 2008 7:48 AM -0600 Walters, Katherine S
katherine-walt...@uiowa.edu wrote:
This may be another silly question, but how does one test the
concentration of formaldehyde in solution?
Thanks,
Kathy
Notice: This UI Health Care
the sample was not adequately fixed.
there i will get off my Friday soap box..
Happy Holidays to all!
Patsy
Original Message
Subject: RE: [Histonet] Silly Question?
From: Merced Leiker lei...@buffalo.edu
Date: Fri, December 12, 2008 8:12 am
To: Edwards, R.E. r
So...is a polymer of paraformaldehyde considered depolymerized if it
remains somewhere between 1-50 molecules long once it's been dissolved in
solution, however it's dissolved? (the dissolving being a separate topic of
debate on Histonet). Does it matter for tissue fixation purposes if there
Does anyone have any suggestions for staining PCNA on paraffin? We are
using Santa Cruz's PCNA (FL261) SC-7907.
We have already stained with it successfully on frozen sections, but it
does not appear to be working on paraffin. Thank you.
Merced M Leiker
Research Technician II
354 BRB (pkgs)
We routinely add paraformaldehyde to alkaline water at room temperature
while stirring and wait only about 30-60 mintues for it to dissolve. Then
we add a concentrated amount of PBS up to the total required volume (so
that the buffer is 1x in the final volume). Then we add acid to bring the
Sounds like you found out the hard way?
--On Wednesday, December 03, 2008 6:07 PM -0600 Ingles Claire
[EMAIL PROTECTED] wrote:
FYI Everyone:
If you are still waiting for your inspection, don't bother putting up
Christmas decorations. Claire
___
At our university in Buffalo, NY, we have to sign out the alcohol we
purchase from the university (we could buy it from Sigma or other places, I
suppose, but generally want to avoid the SH fee). I was told the State of
NY required the signature...why? So if we're caught driving drunk they can
Ahhh...they are federally regulated...
http://www.sigmaaldrich.com/chemistry/solvents/products.html?TablePage=14577624
--On Friday, November 07, 2008 9:29 AM -0500 Merced Leiker
[EMAIL PROTECTED] wrote:
At our university in Buffalo, NY, we have to sign out the alcohol we
purchase from
This cleaning-bones discussion keeps getting better and better! What an
excellent topic for this Halloween week!
--On Monday, October 27, 2008 10:19 AM +1100 Farish, Craig
[EMAIL PROTECTED] wrote:
Hi Ian,
Dermestid beetles are definitely the least smelly option
(although
We need this kind of Society in Buffalo, NY. This sounds like too much fun.
--On Thursday, October 23, 2008 4:10 PM -0400 Kristen Yaros
[EMAIL PROTECTED] wrote:
This is a reminder to all Delaware members and non-members that we will be
holding our open meeting and Hayride/Bonfire at
3ug/ml sounds awful low for a stock concentration...
--On Friday, October 24, 2008 11:51 AM -0500 Swain, Frances L
[EMAIL PROTECTED] wrote:
Hi bert ifyou will look at the image for the antibody for IHC of MAOB you
will see at the bottom that the antibody demonstrated was at a
concentration
...considering the stock Ab concentration isn't even known, as Brett
mentioned.
--On Friday, October 24, 2008 1:09 PM -0400 Merced Leiker
[EMAIL PROTECTED] wrote:
3ug/ml sounds awful low for a stock concentration...
--On Friday, October 24, 2008 11:51 AM -0500 Swain, Frances L
[EMAIL
Hi Ann,
Our lab purchased a Leica 2030 microtome (using disposable blades)through
Medical Equipment Source. I'd looked at several companies that sell
refurbished microtomes, and went with this company because of the cost
($3000) that included a warranty and a newer knife holder than the
Thanks to everyone who replied to my query! I received many insightful
responses.
Merced
--On Thursday, October 09, 2008 5:19 PM -0400 Merced Leiker
[EMAIL PROTECTED] wrote:
What are people's exerience with manual processing of FFPE tissues using
a microwave? My boss has me looking
Hi James,
While I know that others with more experience are going to reply and have
very good insights to add, in my few years of experience I've stored the
paraffin sections at room temp. for up to several months. Sections are
stored only after baking them in a 60 C for 1 hour. This baking
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