[Histonet] Much off from normal histology topics

So please delete as quickly as you want. Happy Memorial Day and everlasting thanks to those who gave their all. Just returned last week from the International Science and Engineering Fair in Los Angeles. 1,700 high school students from 75 countries and regions with their 1,400 projects. Not

Re: [Histonet] low signal for long post fixation

Mariela, (1) If you are looking at enzyme activity of a LacZ transgene to localize with X-gal staining, I found the situation really a problem with extended fixation times of vibratome sections (like you with controls or test article). Have never been able to "retrieve" that kind of staining.

Re: [Histonet] Histonet Digest, Vol 160, Issue 1

All, I think John Frazier is exactly spot-on with his e-mail. This is not a single variable equation solved easily. It is complex and not so easy as it might seem for a recycler sales pitch. I am FOR recycling and the environment whenever it is at all possible in any situation but there is

Re: [Histonet] Frog tissue

Hello, For years and years in past, I did the very same thing for a middle school science teacher before, during and after son was there. Don't know what level this is at so unless all the kids are interested in histology, I've found no need to go too far with this (are they really going to

Re: [Histonet] Santa Cruz antibodies

Pretty interesting Erin, thanks. I reread the Nature article I saw back in February detailing a bit about the complaints being investigated.  Among those complaints were notifications of goats with coyote bites and massive tumors (from where??).  Both of which could drastically affect

Re: [Histonet] thank you HistoNet! Histology STEM long off-topic

Histonet-   I cringe at the thought of being dinged for taking time with something somewhat tangential to histology but here I go.  If histology as part of STEM (science, technology, engineering, math) and kids' futures is a bit overboard for you, please use your delete button now.   First

Re: [Histonet] PAS Stain

Indeed, a very curious and interesting way to do this.  And as I said-have done it very occasionally in long ago past. What I am still curious about is that for those who still do this, how do you write it up for CLIA or CAP or GLP when, as the Samuri Pathologist would call them, Herrn

Re: [Histonet] PAS Stain

An excellent point.  For anyone wanting to investigate-simply do a PubMed search on variation of AMY1 gene.  Sorry; I guess I should say this is, strictly speaking, non-histology related topic and I don't want to get into trouble as some before me.  Tons of research about this linking back (in

Re: [Histonet] PAS Stain

Hello   Agree with comments about temperature of 60 degrees killing an enzyme.  If you plot enzyme activity on "y"  axis and temperature on "x" axis it is not a straight line.  Every enzyme has an optimal temperature and can function slowly at non-optimal or optimally at correct temperature. 

Re: [Histonet] PAS Stain

I love having the Samuri Pathologist on this forum for wisdom and real-laboratory life knowledge.  And yes, I have in the past spit on slide ON OCCASSION when faced with a dire necessity.  Although I know there are those who would wretch about this; it remains a fact of viable laboratory life

Re: [Histonet] Double stain IHC question

All, I guess I'm reading (or misreading) the situation differently than some.  That this is frozen, and not FFPE, is given originally.   But I'm reading this as two different antibodies marking the very same protein. [Antibody=same protein A].  Is the original question can you mark same

Re: [Histonet] Pas Digestion

As Jennifer mentioned, enzymes have optimal temperatures at which to work.  But also optimal pH.  Is your 0.5% amylase in water or a correct buffer? Ray, Seattle, WA - Original Message - From: "Loree via Histonet Lager" To:

Re: [Histonet] Getting rid of bubbles in a gelatinous slide mounting medium

Have never used the following method for gelatin microscope slides but maybe it would work  When pouring acrylamide gels or setting up a resin matrix column I would always de-gas the liquid in a vacuum set-up of some kind.  Placing a liquid in the vacuum not only gets rid of oxygen which