Hello Techs,
I would like to get some feedback on the Dako Autostainer Link 48, using the
Flex reagents.
Thank you,
Cindy
Cynthia Bulmer HT(ASCP)QIHC
IHC Supervisor, CTPL
Waco, TX
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
I love it. Great turn around time. Great crisp clean stains. And I think it's
easy to use. Some will say ah it has the off line Retrieval. But I like the
fact that the samples are submerged into a solution. Call me old fashioned?
I've used pretty much all of the platforms. They all have good
Anyone have a Protocol for Decalcified bone for IHC?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
cool !!
On Mon, Mar 12, 2012 at 8:49 PM, Jeffery Howery jeffery.how...@jcl.comwrote:
Anyone have a Protocol for Decalcified bone for IHC?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Having problems with IHC slides not looking the same between runs. Does it have
to do with how long and at what temp they are in the oven for?
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the new health
610 Oakmont Lane
Westmont, IL 60559
Office: +
Not really. Time in the oven and temp is usually not an issue as long as the
tissue is well fixed/processed/infiltrated.
René J.
--- On Mon, 3/12/12, Courtney Pierce courtney.pie...@quintiles.com wrote:
From: Courtney Pierce courtney.pie...@quintiles.com
Subject: [Histonet] IHC Slides
Posting for a colleague:
Which of the following choices
Cytokeratin (NOS)
Vimentin
s-100
Cd45
Desmin
Would be the best to use as an internal positive control for fixation and
processing?
Any supporting literature/references/docuemntation would be very helpful...
Thanks
Luis
My vote has always been for vimentin. See Dako's booklet IHC Staining Methods,
5th Ed, Chapter 19 addresses this.
Angie
Chiriboga, Luis luis.chirib...@nyumc.org 3/6/2012 9:01 AM
Posting for a colleague:
Which of the following choices
Cytokeratin (NOS)
Vimentin
s-100
Cd45
Desmin
Would be
Vimentin
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Chiriboga, Luis
Sent: Tuesday, March 06, 2012 8:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC + control question
Posting
Vimentin
Williams et al.(1997); J.Clin.Pathol.,50:422-8
René J.
--- On Tue, 3/6/12, Chiriboga, Luis luis.chirib...@nyumc.org wrote:
From: Chiriboga, Luis luis.chirib...@nyumc.org
Subject: [Histonet] IHC + control question
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
I know that many labs run Vimentin for this, but please give consideration to
running a nuclear marker as well especially since some of our predictive
markers (ER and PR) are nuclear proteins. In my opinion, Vimentin is very
robust and may show-up even when the tissue is not adequately fixed
Onderwerp: [Histonet] IHC and negative controls?
Hi All,
We have one Ventana XT and often exceed the slide capacity. Our pathologists
no longer want us to run negatives and will use internal negatives instead. Is
this common practice? Thanks. I appreciate any feedback.
Tom McNemar, HT(ASCP
Hi All,
We have one Ventana XT and often exceed the slide capacity. Our pathologists
no longer want us to run negatives and will use internal negatives instead. Is
this common practice? Thanks. I appreciate any feedback.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health
Histologist, Biomimetic Therapeutics, Inc.
From: dphill...@vetmed.lsu.edu
To: Histonet@lists.utsouthwestern.edu
Date: Mon, 13 Feb 2012 09:53:15 -0600
CC:
Subject: [Histonet] IHC and bone implants
Does anyone have any suggestions for staining bone implants? I am having
trouble
Dear all,
We have developed CQPath for our QC/QA. Still under construction at
www.cqpath.nl (of course also in English). Hopefully the TQM module will
be available soon.
Best regards,
Marcel
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or
Hello Histonet,
Wondering what those of you that do IHC on bones do to ensure that the tissue
remains on the slide during processing?
Thanks,
Sara McCabe HT(ASCP)
DuBois Regional Medical Center
This email and any attached files are confidential and intended solely for the
intended
pray.
McCabe, Sara J. sjmcc...@drmc.org 12/30/2011 1:02 PM
Hello Histonet,
Wondering what those of you that do IHC on bones do to ensure that the tissue
remains on the slide during processing?
Thanks,
Sara McCabe HT(ASCP)
DuBois Regional Medical Center
This email and any attached files are
So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a
positive control for both of these at the same time? Will tonsil pop positive
for both?? If not, what are good controls for each of them?
Thanks
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150
Hi: Would anyone have a hereditary nonpolyposis colorectal cancer control,
(for MHL1, MSH2, MSH6) to share?
Thanks in advance,
--
Marian L. Powers
*Doctors Pathology Services * **
c| 302.747.0580
o| 302.677. ext: 110
f | 302.677.0010
___
Hello everyone,
I'm in need for an anti-rat-Serum amyloid P antibody which is IHC-P compatible.
Suggestions, anyone?
Thank you very much in advance!
Best,
Till
GSI Helmholtzzentrum für Schwerionenforschung GmbH
Planckstraße 1
64291 Darmstadt
www.gsi.de
Gesellschaft mit beschränkter
...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Aperghis Kavanagh
Sent: Tuesday, September 27, 2011 1:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC H.P. Negative Control
Hi Histonetters,
We would like to start to run up to 30 H.Pylori on our Benchmark daily. Do GI
labs that run H.P. on ALL
12:16 PM
To: Sheila Fonner
Subject: RE: [Histonet] IHC H.P. Negative Control
Sheila,
Cap requires a negative with each block of patient tissue being stained for
immunos, However they also state that if more than one block from the same
patient is being tested that only one of the blocks
Subject: [Histonet] IHC H.P. Negative Control
Hi Histonetters,
We would like to start to run up to 30 H.Pylori on our Benchmark daily. Do GI
labs that run H.P. on ALL stomach biopsies run a negative control for each
patient tissue for H.P.? I am just curious, as we have a 24 hour TAT
Hi Histonetters,
We would like to start to run up to 30 H.Pylori on our Benchmark daily. Do GI
labs that run H.P. on ALL stomach biopsies run a negative control for each
patient tissue for H.P.? I am just curious, as we have a 24 hour TAT, but the
Benchmark only holds 30 slides. What's
Hello all,
So I just realized I am out of my block after I have done HIER. Can I
leave the slides in buffer in the fridge or something overnight. The
block will be here at 10am tomorrow morning...
She the slides be ok or do I need to re-cut all of them and start over
(please tell me this is
Excellent questions, I get it all the time. Overnight, in buffer, in the
fridge will be ok. Do not leave in water as that will obscure morphology.
Will
On Sep 15, 2011, at 12:40 PM, sdys...@mirnarx.com wrote:
Hello all,
So I just realized I am out of my block after I have done HIER.
: [Histonet] IHC ?
To: histonet@lists.utsouthwestern.edu
Date: Thursday, September 15, 2011, 3:40 PM
Hello all,
So I just realized I am out of my block after I have done HIER. Can I
leave the slides in buffer in the fridge or something overnight. The
block will be here at 10am tomorrow morning
which antibody did well in xp11.3 renal cancer?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Does anyone offer immunohistochemical testing for the Y-encoded TSPY protein
in testicular biopsy tissue? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80
Hello Histo Friends,
Has anyone had to validate IHC antibodies when you do not have tissue processed
in house to use for your validation. I do have control tissue that stains
properly but it was acquired from outsides sources with verified proper
predicted staining.
If you have done this
, CA, USA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Thursday, September 01, 2011 9:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC validation
Hello Histo Friends
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Oneil,
Beth Ann
Sent: Thursday, August 18, 2011 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC control material
We would like to re-organize our system for maintaining blocks
Good morning everyone,
I was wondering if anyone could help me with a protocol change for the
Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll, and
Zoster Virus on frozen tissue that was received for IF studies. I know it's
not Monday, but it is early, and I was hoping
Thank you.
-Original Message-
From: Mierow, Brett T. [mailto:brett.mie...@essentiahealth.org]
Sent: Tuesday, August 16, 2011 7:02 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue
You are correct, leave out the depar. step.
Brett Mierow, HT (ASCP)
Histology
Does anyone else leave out the cell conditioning step?
-Original Message-
From: Yahoo [mailto:kim.tourn...@yahoo.com]
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
I would leave out the pretreatment step as well i.e. Enzyme, CC1
Jan,
Thanks, I really appreciate the help. This is my first attempt, so we'll
see how it turns out. :)
Sheila
-Original Message-
From: Jan Shivers [mailto:shive...@umn.edu]
Sent: Tuesday, August 16, 2011 9:21 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
Sheila
We've had excellent service from Ventana and they do have to travel from
usually Chicago up to Madison.
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Inman, Anna
Sent: Mon 8/1/2011 11:30 AM
To: histonet@lists.utsouthwestern.edu.
Subject: [Histonet
Can you tell me what kind of on-site technical service you are
accustomed to receiving from Ventana (especially if you NOT in a big
city so travel is required)? We have had a terrible time getting a
Field service engineer into our lab this year and wonder if that is the
norm for Ventana these
Hi All, was wondering how everyone does their IHC for H. pylori. Do you
automatically run it on all biopsies of the stomach, or do you screen first
with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for
the input!
Joanne Clark, HT
Histology Supervisor
Pathology
Our pathology groups decision was to run IHC for H.pylori on all stomach
biopsies.
On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark jcl...@pcnm.com wrote:
Hi All, was wondering how everyone does their IHC for H. pylori. Do you
automatically run it on all biopsies of the stomach, or do you
We only run IHC for H. pylori when the appropriate inflammatory background is
present or when the patient has tested positive in the past and we receive a
follow-up gastric specimen where bugs are not identified on HE.
Running H. pylori IHC on every gastric biopsy is uncalled for and borders on
We run the H. pylori IHC stain on all stomach specimens to improve turn
around time; however, when an inflammatory background is not appreciated by
the pathologist the charge is removed before sign-out and we eat the cost of
producing the slide.
Mark
On Wed, Jul 13, 2011 at 11:37 AM, Richard
@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC for H. pylori
*** WARNING ***
This email has already passed extensive Anti-Spam and Anti-Virus scanning
measures and found to have a high probability of not containing spam or virus
content.
However... This email has failed Domain Keys Identified
Please help me understand, do we need to verify antibodies or validate them,
or is it essentially the same thing? Also, can I please get a couple
examples of the forms you might be using, perhaps email me a copy???
I'm still new to the IHC world and trying to get things inline as they need
to be.
@lists.utsouthwestern.edu
Subject: [Histonet] IHC verification forms
Please help me understand, do we need to verify antibodies or validate them,
or is it essentially the same thing? Also, can I please get a couple
examples of the forms you might be using, perhaps email me a copy???
I'm still new to the IHC world
If you haven't made up your mind yet on this issue, here are a couple of
references that may - or may not - help:
Veterinary Diagnostics:
[mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:31 PM
To: Dawson, Glen; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue
: [Histonet] IHC pos. neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible for this not to be so. However,
with a negative, the concern
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible
@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
And the block in question has already been proven positive using THAT
procedure and antibody during validation.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
20, 2011 11:57 AM
To: Curt Tague
Cc: Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. neg. control question
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Jay
Date: Thu, 19 May 2011 13:25:49 -0400
From: Jean-Martin Lapointe jm.lapoi...@accellab.com
Subject: [Histonet] IHC pos. neg. control question
To: histonet@lists.utsouthwestern.edu
Message-ID:
befd613bd39142499989f836556ddc8301272...@ace.accellab.lan
Content-Type: text/plain; charset
80504
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Friday, May 20, 2011 2:16 PM
To: jm.lapoi...@accellab.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. neg. control
@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. neg. control question
Hi,
This is simply a question of definitions. They are actually both
right.
If you have the patient tissue as a negative control you are not using
the
primary antibody on it but are replacing it (ideally) with an Ig
, Colorado 80504
-Original Message-
From: Amos Brooks [mailto:amosbro...@gmail.com]
Sent: Friday, May 20, 2011 3:05 PM
To: Liz Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. neg. control question
Thanks Liz,
You are absolutly right there, but have you
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we get
one
Curt,
Nonsense. The negative control is used to evaluate endogenous staining
in the *patient* tissue. Your Pathologist needs to do another residency at
clown college.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
] Im Auftrag von
Jean-Martin Lapointe
Gesendet: Donnerstag, 19. Mai 2011 19:26
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] IHC pos. neg. control question
Hi Curt,
I agree with your pathologist. The section that you use as a negative
control (without primary) in an IHC run should
: Thursday, May 19, 2011 12:04 PM
Subject: [Histonet] IHC pos. neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had no
problems. Am I missing something. Is there any documented
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. neg. control question
I got this email from a pathologist today. we
, 2011 1:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense. Because it would not be from
the patient tissue being tested, how do you
I agree 100% with Glen.
Jan Shivers
UMN VDL
- Original Message -
From: Dawson, Glen gdaw...@dynacaremilwaukee.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 19, 2011 1:32 PM
Subject: RE: [Histonet] IHC pos. neg. control question
IMHO: Running any piece of tissue
, Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
patient tissue
...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn't
--
Message: 11
Date: Thu, 19 May 2011 13:37:38 -0500
From: Sebree Linda A lseb...@uwhealth.org
Subject: RE: [Histonet] IHC pos. neg. control question
To: Dawson, Glen gdaw...@dynacaremilwaukee.com,
histonet@lists.utsouthwestern.edu
Message-ID
: Thursday, May 19, 2011 4:50 PM
To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. neg. control question
I agree with the majority. A patient slide stained without primary antibody,
and a patient section with primary antibody and a known positive control
...@copc.net]
Sent: Thursday, May 19, 2011 1:39 PM
To: Pedersen Pete; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running
[mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:54 PM
To: Thomas Jasper; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
Thomas,
Agreed, however, how can you say with certainty that the control is
still good, or the antibody is still
Curt is right, according to CAP ...
ANP.22570 QC - Antibodies Phase II
Appropriate negative controls are used.
NOTE: Negative controls must assess the presence of nonspecific staining in
patient tissue as well
as the specificity of each antibody. Results of controls must be documented,
...@lists.utsouthwestern.edu] On Behalf Of Jean-Martin
Lapointe
Sent: Friday, 20 May 2011 7:50 AM
To: Angela Bitting; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
I can certainly agree with that. Whether it’s the patient’s sample or the known
positive control
hai histonetter,
i am doing ihc on mouse femour(bone marrow),i am using nucleostemin
antibody to locate cells in proliferation, can any one tried this if u tried
please give me the idea about nucleostemin ihc on mouse femour, thank u.
M.Manikandan,
Researcher,
Stemcell unit,
King Saud
Does everyone print out IHC run reports after each run? If so, how long
do you keep them and are the pathologists supposed to sign off on them
with their slides?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC run print outs
Does everyone print out IHC run reports after each run? If so, how long
do you keep them and are the pathologists supposed to sign off on them
with their slides
] On Behalf Of WILLIAM DESALVO
Sent: Wednesday, May 11, 2011 11:09
To: amber.mcken...@gastrodocs.net; histonet
Subject: RE: [Histonet] IHC run print outs
To meet CAP requirements, we print un reports and send send to the pathologists
with slides, they review, sign off and we keep for 2 yrs. All QC
...@gastrodocs.net
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, May 11, 2011 11:01 AM
Subject: [Histonet] IHC run print outs
Does everyone print out IHC run reports after each run? If so, how long
do you keep them and are the pathologists supposed to sign off on them
with their slides
@lists.utsouthwestern.edu
Subject: [Histonet] IHC run print outs
Does everyone print out IHC run reports after each run? If so, how long
do you keep them and are the pathologists supposed to sign off on them
with their slides
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Amber
McKenzie
Gesendet: Mittwoch, 11. Mai 2011 17:01
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] IHC run print outs
Does everyone print out IHC run reports after each run? If so, how long
do you keep them and are the pathologists
Nashville, TN 37232
Message: 10
Date: Tue, 19 Apr 2011 14:04:32 -0500
From: Ring, Mary L mary.l.r...@healthpartners.com
Subject: [Histonet] IHC validation
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
2594d39b69223047b6e2f4adf1e62db9010ffb487...@hpemx3
Would anyone be willing to share their IHC menu with us? I want to see how we
compare with other top-class facilities. Obviously I am more interested in
pediatric facilities, but we are constantly evaluating antibodies for research
studies, and what may have applications in pediatric pathology
Following is question on behalf of Jana Moose Ritter, DVM
janamo...@yahoo.com
We are going to perform IHC on frozen sections, and the tissues (pig
aorta) will be harvested on a Friday.
Histo lab is wondering:
1) can tissue fix in paraformaldehyde over the weekend until next
Does a change in the type of hematoxylin counterstain require any re-validation
of IHC stains?
Thanks for your help!
Mary Ring, HT, QIHC
Regions Hospital, St Paul, Mn
This e-mail and any files transmitted with it are confidential and are intended
solely for the
mary.l.r...@healthpartners.com wrote:
From: Ring, Mary L mary.l.r...@healthpartners.com
Subject: [Histonet] IHC validation
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, April 19, 2011, 3:04 PM
Does a change in the type of hematoxylin counterstain require any
In my opinion, only if it affects immunoreactivity.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860)
Hello,
We are inquiring about IHC staining platforms and we've already contacted
the Leica sales rep for the Bond. I would also like to contact Ventana to
get a quote on their system, and I've tried to get in contact with a sales
rep in our area but I haven't had anyone returning my calls.
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are currently in antigen retrieval that has come to room
temperature. I am not going to be able to finish the IHC stains until
tomorrow. Will it be better to keep the slides in the antigen retrieval
solution at 4
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC slides
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are currently
You can put them %3 H2O2 for 10 minutes. and then drop primary antibody and
incubate at 4 degrees overnight.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Just hold them in buffer overnight.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
In buffer at 4ºC overnight
René J.
--- On Wed, 3/2/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.com sgoe...@mirnarx.com
Subject: [Histonet] IHC slides
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 2, 2011, 2:24 PM
So...I have run down slides and done
...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Wednesday, February 23, 2011 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Equipment
Hi Histonetters
I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model
: Wednesday, February 23, 2011 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Equipment
Hi Histonetters
I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model that we may need to replace in the
near future. What
Of Cynthia
Pyse
Sent: Wednesday, February 23, 2011 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Equipment
Hi Histonetters
I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model that we may need to replace
25, 2011 9:31 AM
To: Sheila Fonner; histonet@lists.utsouthwestern.edu; 'Cynthia Pyse'
Subject: RE: [Histonet] IHC Equipment
The Bond does do ISH. In fact it utilizes the same detection kit so you have
only to buy the probes, not the additional detection kit that could
(depending on your volume
; Cynthia Pyse
Subject: Re: [Histonet] IHC Equipment
In regards to the Ventana Ultra - if you are running PIN-4 or ISH
simultaneously on this instrument, the kits/ab's/probes they require take
up a lot of space on the reagent carousel which limits the number of
antibodies you can put on and as runs
: [Histonet] IHC Equipment
In regards to the Ventana Ultra - if you are running PIN-4 or ISH
simultaneously on this instrument, the kits/ab's/probes they require take
up a lot of space on the reagent carousel which limits the number of
antibodies you can put on and as runs can go some where around 6
Hi Histonetters
I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model that we may need to replace in the
near future. What is everyone using out in histoland. I would be perfectly
willing to purchase another Dako but I want to
I recently came across a protocol that was conducting IHC staining for pSTAT3 on
free-floating fixed rat brain sections. The protocol stated that the tissue was
placed in 1% NaOH and 1% H2O2 in H2O for 20 min, 0.3% glycine for 10 min, and
0.03% sodium dodecyl sulfate for 10 min.
Could someone
Subject
02/09/2011 03:26 Re: [Histonet] IHC validation
PM
...@sjha.org
Subject: RE: [Histonet] IHC validation
To: Liz Chlipala l...@premierlab.com, Joe Nocito
jnoc...@satx.rr.com, Histonet histonet@lists.utsouthwestern.edu
Message-ID:
92ad9b20a6c38c4587a9febe3a30e164081e09a...@chexcms10.one.ads.che.org
Content-Type: text/plain; charset=us
401 - 500 of 722 matches
Mail list logo
