Re: [Histonet] Inconsistent H & E Staining

2018-11-15 Thread John Garratt via Histonet
A quick question: Are you using any recycled reagents? ie xylene, alcohol John www.ciqc.ca ‐‐‐ Original Message ‐‐‐ On Wednesday, November 14, 2018 12:24 PM, MONICA D. LOCKHART via Histonet wrote: > Hello Histo Fam!! > > Please help! > > My facility is experiencing frequent H & E

Re: [Histonet] Inconsistent H & E Staining

2018-11-14 Thread Caroline Miller via Histonet
Have you checked your dewaxing solutions? I have sometimes seen this when slides are not fully dewaxed. yours, mills ᐧ On Wed, Nov 14, 2018 at 12:34 PM MONICA D. LOCKHART via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histo Fam!! > > Please help! > > My facility is

[Histonet] Inconsistent H & E Staining

2018-11-14 Thread MONICA D. LOCKHART via Histonet
Hello Histo Fam!! Please help! My facility is experiencing frequent H & E staining problems. Everything from cloudy cells on routine GI to "The Blank Look" on PNB's. We have made changes in some of the obvious areas like cassette whole size and discontinued the use of sponges at grossing.